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Cytoskeleton/SUMO1 IP Control Beads/12 reactions/CIG03-beads
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Details As part of the Signal-Seeker™ product line, SUMO1 IP control beads (CIG03) have been developed as highly specific negative control beads for Cytoskeleton"s SUMOylation 1 antibody-based affinity bead products. These control beads are far superior to beads alone, because it is a better representative of the actual IP condition. The IgG antibodies are covalently attached to minimize antibody heavy and light chain leaching, which makes interpretation of results easier. Additionally, these control beads are effective for pre-clearing lysates without risk of contamination with IgG antibody. IgG control beads (CIG03) are included in our comprehensive Signal-Seeker™ SUMOylation 1 Detection Kits and utilizing these kits are recommend for first time SUMO1 detectiion.Each lot of affinity-bead is quality controlled to provide high batch to batch consistency, see COA documents. Validated Applications Application 1: Utilization of acetyl-lysine control beadsSUMO1 IP control beads are compatible with SUMOylation 1 Affinity Beads and Signal-Seeker SUMOylation 1 Detection Kits. Note these beads do not have cross reactivity with SUMO1 (Figure 1).Figure 1: HAP1 wildtype (WT) or SUMO1 knockout (KO) lysate, was obtained using BlastR lysis and filter system. 1 mg of each lysate were incubated with 40 µg of SUMO1 affinity beads (ASM11-beads (Cytoskeleton)), and conjugated SUMO1 IP control beads (CIG03). Samples were separated by SDS-PAGE and transferred to PVDF. Enriched SUMO1 samples were analyzed by western blot using SUMO1 (ASM01 (Cytoskeleton)) antibody at 1:5000. Mouse-HRP secondary at 1:20,000 in 5% milk was used.Amount:Each package contains enough IgG control beads for 12 reactions. For more information contact: signalseeker@cytoskeleton.comAssociated Products:Signal-Seeker™ SUMOylation 1 Detection Kit (Cat. # BK165)Signal-Seeker™ SUMO1 Affinity Beads (Cat.# ASM11-beads)Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)AboutClick on the pdf icon below to download the datasheet CitationsNew product released in 2018! For the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click hereFaqsVisit our Signal-Seeker™ Tech Tips and FAQs page for technical tips and frequently asked questions regarding this and other Signal-Seeker™ products click here If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
Cytoskeleton，Inc.成立于1993年。自成立以来，我们一直在不断扩大产品范围。
Cytoskeleton，Inc.很高兴为药物筛选，信号转导和细胞骨架研究提供广泛的试剂盒和产品。我们专注于纯化蛋白的生产和易于使用的试剂盒，以研究生化和细胞过程。我们的试剂盒既可用于基础研究或小型筛查的少量样品，也可用于大型筛查的高通量规模
除了我们现有的产品外，我们还提供产品系列中微管，微管蛋白，运动蛋白，小G蛋白效应物，GAP，GEF和其他几种蛋白的药物筛选服务。有关更多信息，请参见我们的药物筛选服务页面。
如果您想从市场上购买到特定产品，请随时与我们联系。我们在这里为您提供帮助。
由于我们的科学家在各自的专业领域都有多年的工作经验，因此我们能够提供高质量的产品。自1993年成立以来，我们以合理的价格生产优质的产品而闻名。
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