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Cytoskeleton/Actin polymerization buffer: 10x stock/1x2 ml/BSA02
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DetailsProduct Uses IncludePolymerization of G-actin into F-actin MaterialThis is a 10 x solution that contains 500 mM KCl, 20 mM MgCl2 and 10 mM ATP in 100 mM Tris, pH 7.5. It is used to add to G-actin solutions in A-buffer (General actin buffer (Cat. # BSA01), 0.2 mM ATP (Cat. # BSA04) and 0.5 mM DTT) to induce polymerization into F-actin.AboutFor product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.comCitationsXiao et al., 2013. c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution. Am. J. Physiol. Endocrinol. Metab. 304, E145-E159.Butler et al., 2012. Inhibitory effects of pectenotoxins from marine algae on the polymerization of various actin isoforms. Toxicol. In Vitro. 26, 493-499.Jiwani et al., 2012. Chlamydia trachomatis Tarp cooperates with the Arp2/3 complex to increase the rate of actin polymerization. Biochem. Biophys. Res. Commun. 420, 816-821.Fan et al., 2012. A role for γS-crystallin in the organization of actin and fiber cell maturation in the mouse lens. FEBS. J. 279, 2892-2904.Tsai et al., 2011. 7-Chloro-6-piperidin-1-yl-quinoline-5,8-dione (PT-262), a novel ROCK inhibitor blocks cytoskeleton function and cell migration. Biochem. Pharmacol. 81, 856-865.Trigili et al., 2011. Mechanism of Action of the Cytotoxic Macrolides Amphidinolide X and J. ChemBioChem.12, 1027-1030.Takamiya et al., 2005. Overexpression of mutated Cu,Zn-SOD in neuroblastoma cells results in cytoskeletal change. Am. J. Physiol. 288, C253-C259. Kumar et al., 2004. Functional dissection and molecular characterization of calcium-sensitive actin-capping and actin-depolymerizing sites in villin. J. Biol. Chem. 279, 45036-45046. Fontao et al., 2001. The interaction of plectin with actin: evidence for cross-linking of actin filaments by dimerization of the actin-binding domain of plectin. J. Cell Sci. 114, 2065-2076. Zhai et al., 2001. Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeleton. J. Biol. Chem. 276, 36163-36167. Blader et al., 1999. GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro. Mol. Biol. Cell. 10, 581-596.FaqsQuestion 1: Once resuspended, can left-over actin polymerization buffer be stored at 4°C to maintain activity?Answer 1: Upon reconstitution (2.0 ml of 100 mM Tris-HCl pH 7.5), the actin polymerization buffer (Cat. # BSA02) will be at 10X strength. Set aside the volume needed for that day’s experiments (This buffer is stable for 30 min on ice) and aliquot the remainder into 100 μl volumes, snap frozen in liquid nitrogen, and stored at -70°C. These stocks are stable for 6 months at -70°C.Question 2: After resuspension, does the actin polymerization buffer need to be used in a certain amount of time before losing activity?Answer 2: The actin polymerization buffer (Cat. # BSA02) will be stable for 30 min after thawing the previously frozen aliquots (or using freshly prepared). Keep on ice until ready to use. We do not recommend re-freezing aliquots.If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
Cytoskeleton，Inc.成立于1993年。自成立以来，我们一直在不断扩大产品范围。
Cytoskeleton，Inc.很高兴为药物筛选，信号转导和细胞骨架研究提供广泛的试剂盒和产品。我们专注于纯化蛋白的生产和易于使用的试剂盒，以研究生化和细胞过程。我们的试剂盒既可用于基础研究或小型筛查的少量样品，也可用于大型筛查的高通量规模
除了我们现有的产品外，我们还提供产品系列中微管，微管蛋白，运动蛋白，小G蛋白效应物，GAP，GEF和其他几种蛋白的药物筛选服务。有关更多信息，请参见我们的药物筛选服务页面。
如果您想从市场上购买到特定产品，请随时与我们联系。我们在这里为您提供帮助。
由于我们的科学家在各自的专业领域都有多年的工作经验，因此我们能够提供高质量的产品。自1993年成立以来，我们以合理的价格生产优质的产品而闻名。
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