Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.    Western blot was performed using Anti-beta Catenin Polyclonal Antibody (Product # PA1-37255) and a 87 kDa band corresponding to Catenin beta-1 was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), MCF7 treated with 10 ng/mL of TGF-ß1 for 24 hours (Lane 2), A-431(Lane 3), Hep G2 (Lane 4), HeLa (Lane 5), SK-MEL-31 (Lane 6) and Mouse lung (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).   Immunofluorescence analysis of Catenin beta-1 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with beta Catenin Polyclonal Antibody (Product # PA1-37255) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing membrane localization. Panel e represents decreased expression of MCF7 cells treated with 10 ng/mL of TGF-ß1 for 24 hours. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.   IP-MS enrichment of CTNNB1 (LFQ intensity): CTNNB1 was enriched 301-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and CTNNB1 antibody (Product # PA1-37255). STRING database was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}   Detection of altered expression of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of beta Catenin Polyclonal Antibody (Product # PA1-37255) shows decreased expression of beta catenin upon treatment with 10 ng/mL of TGF-ß1 for 24 hours. {TM}   Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using of beta Catenin Polyclonal Antibody (Product # PA1-37255) shows decreased expression of beta catenin in MCF7 cell line upon treatment with 10 ng/mL of TGF-ß1 for 24 hrs. {TM} 保存条件 Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. This antibody is predicted to react with bovine, amphibian, mouse and rat based on sequence homology.rrHeat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is breast carcinoma. Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin. Have you cited this product in a publication? Let us know so we can reference it here. Cite this product 蛋白别名: armadillo homolog; Bcatenin; beta catenin; Beta-catenin; Betacatenin; C20orf33; catenin; catenin (cadherin associated protein), beta 1, 88kDa; catenin (cadherin-associated protein), beta 1; catenin (cadherin-associated protein), beta 1, 88kDa; Catenin b 1; Catenin b1; catenin beta; Catenin beta-1; Catenin beta1; Catenin β 1; Catenin β1; CTNB1; dJ633O20.1; DKFZp686D02253; FLJ25606; FLJ37923; NYD-SP19; OTTHUMP00000209289; P14L; PP8304; RP5-1118M15.1; β catenin; βcatenin 基因别名: armadillo; B-catenin; beta-catenin; Bfc; Catnb; CHBCAT; CTNNB; CTNNB1; ctnnb1-a; ctnnb1-b; Mesc; MRD19; OK/SW-cl.35; PRO2286; XELAEV_18031149mg Host server : magellan-srch-2-prod-green:8080/10.253.227.78:8080. git-commit: 2cd8645d2fc6bfe4ccb4abfa14772b0a94f68e98 git-url: http://victoria.invitrogen.com:8333/magellan/core.git git-branch: origin/release/1.27.0-2021.08.32-1.0