Description: MPO, Mouse, mAb B7 The monoclonal antibody B7 recognizes human myeloperoxidase (MPO), an ~135 glycoprotein expressed in all cells of the myeloid linage. MPO functions as an α2β2 heteromultimer consisting of two heavy (α) and two light (β) chains of 55 and 15 kDa respectively. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils (PMNs). It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent oxidant. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Upon activation of neutrophils, MPO can be rapidly released and as such useful in body fluids as marker for inflammatory status. Involvement of MPO has been described in numerous diseases such as atherosclerosis, lung cancer, Alzheimer's disease, inflammatory bowel disease and multiple sclerosis. Autoimmune antibodies to MPO (so called ANCA) are involved in Wegener’s disease. Since the discovery of MPO deficiency, initially regarded as rare and restricted to patients suffering from severe infections, MPO has attracted more clinical attention. Specifications: Catalog number HM1135 Product type Monoclonal antibodies Quantity 100 µg Formulation 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide. Application Flow cytometry, Frozen sections, Immuno assays, Western blot Use For Immuno assays, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Immunogen Mouse MPO Isotype Mouse IgG1 Species Mouse Cross reactivity Human - Yes, Rat - Yes Alias Myeloperoxidase Positive Control Neutrophils, HL-60 cells Negative Control Erythocytes Storage and stability Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label. Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product. Disease Cardiology and metabolism, Pulmonology