首页 > Lucigen > Lucigen/E. cloni 10G ELITE Electrocompetent Cells > 2 × 10^10 cfu/µg/60052-4/48 rxns (SixPacks)

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Lucigen/E. cloni 10G ELITE Electrocompetent Cells > 2 × 10^10 cfu/µg/60052-4/48 rxns (SixPacks)

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Directreplacementsforstandardcloningstrains(e.g.,DH5α™,DH10B™,JM109,TOP10,XL1-Blue,etc.)Optimizedgeneticsforhighyields: phageT1resistant,endonucleaseandrecombinationminus,blue/whitescreening-capable.Availableinarangeofhightransformationefficiencies(5×109to4×1010cfu/µg).Convenientpackagingoptions.Lookingforchemicallycompetentversion?FreeSampleAvailable Lucigen’sE.clonicompetentcellssharethemostusefulgeneticelementsofstandardcloningstrainslikeDH5α™,DH10B™,JM109,TOP10,etc.anddirectlyreplacethemincloningprotocols.However,E.clonielectrocompetentcellsincorporateauniquemanufacturingtechnologythatincreasestransformationefficiency,recombinantyieldsandreliABIlity(Figures1and2),whiledecreasingcosts. Thesecellsprovidesolutionsforawiderangeofapplicationsateconomicalprices.Choiceofstrain:E.cloni10GCompetentCells.Libraryconstruction,cloning,subcloning,andplasmidisolationwithorwithoutblue/whitescreening.E.cloni10GF′CompetentCells.ContaintheF'plasmidforinfectionwithM13toproducessDNA.Choiceofefficiency:E.cloni10GSUPREMEElectrocompetentCells>4×1010cfu/µgpUCDNASUPREMECellshavethehighesttransformationefficiencyavailablefromanysupplier.ChooseSUPREMECellsforthemostdemandingcloningsituations,suchasconstructionoflarge,highcomplexitylibrariesorcloningdifficulttargets,whichrequirethegreatestnumberoftransformantspossIBLe.E.cloni10G&10GF´ELITEElectrocompetentCells>2×1010cfu/µgpUCDNAELITECellshavetwicethetransformationefficiencycomparedto“ultrahighefficiency”cellsfromothersuppliers.ELITECellsprovidelargenumbersoftransformantsfromhard-to-clonefragmentsorlimitedDNAatalowerprice.E.cloni10GCLASSICElectrocompetentCells>5×109cfu/µgpUCDNACLASSICCellsarehighefficiencycellswithasubstantiallylowercostperreaction.Thesecellsarethemosteconomicalchoiceforstandardcloningandlibraryconstruction.10GCLASSICCellsareavailableinlargerpackagesizesforconvenientuseinhighervolumecloningapplications.CustomCompetentCellsBulkLucigencellsoryourstrainofE.coli,madetothehighestpossibletransfectionefficiencywithcustomdispensing!SeeCustomCompetentCellspagefordetailedinformation.Plasmidcloning.E.cloni10GSUPREMECellsproviderecombinantyieldshigherthanthehighestefficiencycellsofferedbyaleADIngsupplier(Figures1and2) Figure1.E.cloni10GSUPREMEElectrocompetentCellsconsistentlyoutperformed“ultrahighefficiency”cellsfromaleadingsupplier.Bothstrainsweretransformedwith10pgofpUC19(n=16). Figure2.TransformationefficiencycomparisonofE.cloni10GSUPREMECells(Lucigen)andMegaDH10Bcells(Invitrogen).Competentcellsweretransformedaccordingtothemanufacturer’sinstructionswiththesamepUC19DNAcontrol(providedwiththeInvitrogencells)Table1.E.cloniCompetentCellsforCloningandLibraryConstructionE.cloniCellLinesTransformationEfficiency(cfu/µgpUCDNA)CloningMethylatedDNABAC,CosmidCloningBlue/WhiteScreening10GSUPREMEElectrocompetent>4×1010YESYESYESwithoutIPTGinduction10GELITEElectrocompetent>2×1010YESNOYESwithoutIPTGinduction10GCLASSICElectrocompetent>5×109YESNOYESwithoutIPTGinduction10GF´ELITEElectrocompetent>2×1010YESNOYESIPTGinductionrequiredEfficienciesE.cloni 10GSUPREMEElectrocompetentCells > 4×1010 cfu/µgpUCDNAE.cloni 10G&10GF´ELITEElectrocompetentCells > 2 × 1010 cfu/µgpUCDNAE.cloni 10GCLASSICElectrocompetentCells > 5 × 109 cfu/µgpUCDNA ORDERINFORMATIONCompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasSOLOs(1transformationpertube),DUOs(2transformationspertube),orSixPacks(6transformationspertube).RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen.

美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。