首页 > Lucigen > Lucigen/MasterAmp™ Extra-Long PCR Kit/MHF9220/50 Reactions

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Lucigen/MasterAmp™ Extra-Long PCR Kit/MHF9220/50 Reactions

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MHF9220

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OptimizePCRamplificationoflongDNAampliconsupto40kbFast,simpleoptimizationstartingwiththecompletekitEasilyamplifyDNAtargetsupto40kblongReducePCR-inducederrorsintheselongampliconswiththishighfidelityDNApolymerasemixTheMasterAmp™Extra-LongPCRKitisacompletesystemforsuccessfulandaccurateone-stepamplificationofDNAsequencesupto~40kb.ThekitcontainstheMasterAmp™Extra-LongDNAPolymeraseMix,aswellasnineMasterAmpExtra-LongPCR2XPreMixeswithdNTPs,buffer,andvaryingamountsofMgCl2andtheMasterAmpPCREnhancerwithBetaine.*Werecommendfirstusingthecompletekit(Cat.No.MHF9220)totestthedifferentMasterMixExtra-Long2XPreMixes(Step1,Fig.1)witheachnewtemplateandprimersettoidentifythebestperformingone.OnePreMixwillamplifyyourlongtemplateandgiveconsistentresultsinallsubsequentamplifications(Step2,Fig.1).OncetheoptimalPreMixisidentifiedcontinuetousethatcombinationofMasterAmpExtra-Long2XPreMixandDNAPolymeraseforallsubsequentamplificationsusingthattemplateDNAandprimerset.Figure1.TwostepstoreliableandconsistentPCRresults.STEP1.FindtheoptimalMasterAmp™Extra-LongPCRPreMix.PerformPCRwithyourtemplate,primers,andtheninePreMixes.STEP2.UsethesameMasterAmp™Extra-LongPCRPreMixtoobtainreliable,consistentPCRofthesamesequence.A.Amplificationofa20-kbregionoflamBDaDNAusingMasterAmp™Extra-LongPCR2XPreMixes(1-9).MasterAmp™Extra-LongPCRPreMix4producedoptimalresults.B.SubsequentPCRofa20-kblambdaDNAregionwiththesameprimerpairandMasterAmp™PreMix4gaveconsistentresults,usingtheoptimalPreMixdeterminedinStep1. Figure2.Extra-longamplificationoflambdaDNAusingtheMasterAmp™Extra-LongPCRKit.PCRconditionswereoptimizedandMasterAmpPremix4wasselectedforPCRof1ngoflambdaDNAineachsample.Resultswereanalyzedona0.5%agarosegel.Ampliconsizeswere30kb(lane1),35kb(lane2)and40kb(lane3).LaneM,5-KbDNAladder. *Coveredbyissuedand/orpendingpatents.MasterAmp™Extra-LongPCRKit;Purchaseofthisproductincludesanimmunityfromsuitunderpatentsspecifiedintheproductinserttouseonlytheamountpurchasedforthepurchaser"sowninternalresearch.Nootherpatentrights(suchas5´NucleaseProcesspatentrights)areconveyedexpressly,byimplication,orbyestoppel.FurtherinformationonpurchasinglicensesmaybeobtainedbycontactingtheDirectorofLicensing,AppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404,USA.

美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。