Description:qPCRMasterMix(Universal)andPrimerPremixonlyKAPALibraryQuantificationKitsforNext-GenerationSequencingOurstandardsdon’tchange.Neithershouldyours.KAPALibraryQuantificationKitscontainallthereagentsneededfortheaccurate,reliableandreproducIBLeqPCR-basedquantificationofNGSlibrariespriortopoolingforcaptureorflowcellamplification.KitscontainKAPASYBR®FASTqPCRMasterMix,optimizedforhigh-performanceSYBRGreenI-basedqPCR.TheengineeredKAPASYBRFASTDNAPolymerasecontainedintheMasterMixamplifiesGC-andAT-richDNAfragmentsofdifferentlengthswithsimilarefficiency,makingittheonlyqPCRMasterMixcapableofaccurateqPCR-basedquantificationofNGSlibraries.*Thepre-dilutedsetofDNAStandardscontainedineachkitiscarefullyquality-controlledtoensurelot-to-lotconsistencyandavoiddatadriftovertime.OptimizedPrimerPremixesensureoptimalPCRefficiency.KitswithDNAStandardsandPrimerPremixesforIllumina® andIonTorrent™sequencers areavailable.Pleasereferencethe InstrumentCompatibilityChart forguidanceoncompatibleplatforms.NEW!ImproveexperimentaldesignswithourTechnicalGuideforIlluminaPlatforms*Dataonfile.ForResearchUseOnly.Notforuseindiagnosticprocedures.ProductHighlightsPrevNextAccuratelyquantifyPCR-competentsequencingtemplatesqPCRonly“counts”thoselibrarymoleculesthatcanbesequencedMoreconsistentclusterdensitiesortemplate-to-beadratiosenableoptimalutilizationofsequencingresourcesImprovepoolingformultiplexedsequencingLibraryconcentrationsdeterminedbyqPCRaremorereliablecomparedtoothermethods*TheengineeredKAPASYBRFASTqPCRMasterMixenablesaccuratepoolingoflibrarieswithextremeGC-contentsorlongerinserts*Eliminatedriftwithcarefullyquality-controlledDNAStandardsPredilutedDNAStandardseliminatequantificationerrorsassociatedwithpreparationofstandardcurvesusingin-housestandards*KAPADNAStandardsundergostrictqualitycontroltoensurelot-to-lotconsistencyandeliminatedatadriftovertimeImprovethroughputwithautomationLibraryquantificationassayiscompatiblewith96-and384-wellformatLibrarydilution,reactionsetupanddataanalysiscanbeautomatedforHTPpipelinesLearnMore aboutKAPAAutomatedSolutions*Dataonfile.RelatedProductsNormalizinglibrariesforpoolingandclusteramplification?CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:KAPAhgDNAQuantificationandQCKitKAPALibraryAmplificationKitsKAPAHyperPrepKitsKAPAStrandedmRNA-SeqKitsApplications:ApplicationsQuantificationoflibrariespriortopoolingformultiplexedsequencingQuantificationofindividuallibrariesorlibrarypoolspriortoclusteramplificationoremPCRQuantificationoflibrariespriortoandafterhybridizationcaptureQualitycontrol,optimizationandtroubleshootingoflibraryconstructionprocessesorworkflowsKitSpecificationsandContents/Storage:KitSpecificationsandContents/StorageKitscanbestoredforupto12months at-20˚C.CompletekitsincludeKAPASYBRFASTqPCRMasterMix(2X),PrimerPremix(10X)andasetof6DNAStandards.ReagentsforqPCR(KAPASYBRFASTqPCRMasterMixandPrimerPremix)andDNAStandards(1–6)arealsosoldseparately.Wherenoted,MasterMixescontaininstrument-specificreferencedyes,whiletheUniversalkitincludesROXHighandROXLow(both50X)separately.Components–Illumina®PlatformComponents–IonTorrent™PlatformSpecificationsSpecDescriptionCompatiblePlatformsIlluminaHiSeq,NextSeq,MiSeq,andGAIIxIonTorrentProtonandPGM454GSFLX+andGSJuniorSOLiD5500seriesLibraryTypeAnyStartingMaterialAnyNGSlibraryreadyforclusteramplificationoremPCRStandardcurveconcentrationrange20–0.0002pMforIlluminalibraries83–0.00083pMforIonTorrentand454libraries10–0.0001pg/µLforSOLiDlibrariesSequencingApplicationsWholeGenomeSequencingWholeExomeSequencingTargetedSequencing(custompanels)RNA-SeqChIP-SeqAmpliconSequencingMethyl-Seq