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MCLAB/Inorganic Pyrophosphatase, Thermostable/TI-OEM/Any Size

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  • ModifiedEnzyme,catalyzesthehydrolysisofinorganicpyrophosphatetoformorthophosphate.

    Description:
    Inorganicpyrophosphatase(PPase)catalyzesthehydrolysisofinorganicpyrophosphatetoformorthophosphate.Itretains100%activityafterincubationat100°Cfor4hours.

    Application:
    -OptimizesPCRthroughtheeliminationofpyrophosphate
    -Catalyzestheconversionofinorganicpyrophosphatetoorthophosphate
    -Removesinhibitingamountsofpyrophosphatesinthereaction

    Source:
    AnE.colistraincarryingaplasmidencodingpyrophosphatasefromtheextremeThermophileThermococcuslitoralis.

    UnitDefinition:
    Oneunitistheamountofenzymethatwillgenerate1µmolofphosphateperminutefrominorganicpyrophosphateunderstandardreactionconditions(a10minutereactionat75°Cin50mMTricine[pH8.5],1mMMgCl2,0.32mMPPi,reactionvolumeof0.5ml).

    Buffer:
    20mMTris-HCl
    100mMKCl
    1mMDithiothreitol
    0.1mMEDTA
    50%Glycerol
    pH8.0@25°C

    RecommendedStorageCondition:-20°C

    Reference:
    Heinonen,J.K.andLahti,R.J.(1981)AnalyticalBiochemistry,113,313-317.


    MCLAB (Molecular Cloning Laboratories) is a leader in DNA sequencingservices and consumables. We are committed to providing the bestservices and products to researchers worldwide. MCLAB's large capacityDNA sequencing services (10,000+ reactions in 24 hours) is well knownfor high quality (up to 1000 bp read length), low cost (as low as$3.50/reaction) and fast turn around time (8~24 hours). Our proprietaryDNA sequencing consumables include NanoPOP (DNA Sequencing and FragmentAnalysis Matrix), NanoPOP Running Buffer, BigDye Dilution Buffer, CARE(Capillary Array Regeneration Excellence) solution, and DNA SizeStandards. In Addition, MCLAB also provides many other services,including gene synthesis, protein expression and purification, and codonmaximization. Other products include PCR, Real-time PCR, frozencompetent yeast, E. coli, as well as novel and traditional enzymes formolecular cloning applications.

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