Pre-coated 96 well plateStandardStandard Dilution BufferBiotinylated Detection AntibodyStreptavidin-HRPHRP DiluentWash BufferChromogenStop SolutionAdhesive Plate Covers Abeta40, Abeta42, ABPP, AICD-50, AICD-57, AICD-59, Alpha-CTF, Alpha-secretase C-terminal fragment, Alzheimer disease amyloid protein, amyloid beta (A4) precursor protein, Amyloid intracellular domain 50, Amyloid intracellular domain 57, Amyloid intracellular domain 59, Amyloid precursor protein, Amyloid-beta A4 protein, Amyloid-beta precursor protein, Amyloid-beta protein 40, Amyloid-beta protein 42, APP, APPI, beta-amyloid peptide, beta-amyloid peptide(1-40), beta-amyloid peptide(1-42), beta-amyloid precursor protein, Beta-APP40, Beta-APP42, Beta-CTF, Beta-secretase C-terminal fragment, C31, C80, C83, C99, Cerebral vascular amyloid peptide, CVAP, Gamma-CTF(50), Gamma-CTF(57), Gamma-CTF(59), Gamma-secretase C-terminal fragment 50, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 59, N-APP, P3(40), P3(42), peptidase nexin-II, PN-II, PreA4, Protease nexin-II, S-APP-alpha, S-APP-beta, Soluble APP-alpha, Soluble APP-beta, testicular tissue protein Li 2 About This Kit The Human Amyloid Beta 42 Ultrasensitive (Hu Abeta 42 US) ELISA quantitates Hu Abeta 42 at ultra-low levels in humantissue culture medium, tissue homogenates and cerebrospinal fluid. The assay will exclusively recognize both natural and recombinant Hu Abeta 42 US.Principle of the methodThe Human Abeta 42 US solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Beta amyloid is a 40–43 amino acid peptide cleaved from amyloid precursor protein by a protease, gamma-secretase. Beta Amyloid (1-40) (Amyloid beta 40 or Abeta 40) together with beta Amyloid (1-42) (Amyloid beta 42 or Abeta 42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the Amyloid beta 40 and Amyloid beta 42 alloforms are major components of the extracellular plaques found in Alzheimer\'s disease brain tissue. Rigorous validationEach manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. Host server : magellan-srch-3-prod-green:8080/10.253.228.100:8080. git-commit: 2cd8645d2fc6bfe4ccb4abfa14772b0a94f68e98 git-url: http://victoria.invitrogen.com:8333/magellan/core.git git-branch: origin/release/1.27.0-2021.08.32-1.0