Antigen/Purification: Collapse
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr23/25 of human REDD1.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: Collapse
REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli (Schwarzer et al, 2005). REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress (Katiyar et al, 2009). It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A–DDB1–ROC1–b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr25, Thr23 and Ser19 through the activity of GSK3b (Katiyar et al, 2009).
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
General References
Schwarzer r, Tondera D, Arnold W, Giese K, Klippel A, Kaufmann J (2005) REDD1 integrates hypoxia-mediated survival signaling downstream of phosphatidylinositol 3-kinase. Oncogene 24(7): 1138-49.
Katiyar S, Liu E, Knutzen CA, Lang ES, Lombardo CR, Sankar S, Toth JI, Petroski MD, Ronai Z, Chiang GC (2009) REDD1, an inhibitor of mTOR signaling, is regulated by the CUL4A-DDB1 ubiquitin ligase. EMBO Rep 10(8):866-72.
