Discovery & Translational ResearchCustom LNA Oligonucleotides2DNA & RNA Purification4ISH & Northern Blotting7Lab Essentials1Next-Generation Sequencing5PCR/qPCR/dPCR1Sample Collection & Stabilization0Custom LNA OligonucleotidesPrint For experiments requiring custom-designed, LNA-enhanced oligonucleotides Customizeyour own LNA oligonucleotides Use your own design or let QIAGEN"s LNA experts design them for you Select from a wide range of labels and modifications Benefit from the easy-to-use online design toolCustom LNA Oligonucleotides are ideal for studies involving short or very similar sequences. The high affinity of an LNA-enhanced oligonucleotide to its complementary sequence results in dramatically improved specificity and sensitivity, when compared with traditional DNA or RNA oligos. In many cases, LNA-enhanced oligonucleotides can be used to distinguish between sequences differing by only a single nucleotide, a feature that can be critical for the success of many experiments. 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Just contact us!For Custom LNA Oligonucleotide Large Scale and Custom LNA Oligonucleotide Manual Design, pleasecontact us for ordering and for further information, or email ourSupport Team directly.Buy ProductsProduct DetailsProduct ResourcesBuy ProductsCat No./ID:339400Custom LNA Oligonucleotide (25 nmol synthesis)Go to GeneGlobe25 nmolsynthesis Custom LNA Oligonucleotide; various purification and labeling options availableCat No./ID:339405Custom LNA Oligonucleotide Manual Design (25 nmol synthesis)Go to GeneGlobeCustom LNA Oligonucleotide Manual Design, designed by QIAGEN"s LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 25 nmolCat No./ID:339406Custom LNA Oligonucleotide Manual Design (100 nmol synthesis)Go to GeneGlobeCustom LNA Oligonucleotide Manual Design, designed by QIAGEN"s LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 100 nmolCat No./ID:339407Custom LNA Oligonucleotide Manual Design (250 nmol synthesis)Go to GeneGlobeCustom LNA Oligonucleotide Manual Design, designed by QIAGEN"s LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 250 nmolCat No./ID:339408Custom LNA Oligonucleotide Manual Design (1 µmol synthesis)Go to GeneGlobeCustom LNA Oligonucleotide Manual Design, designed by QIAGEN"s LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 1 µmolCat No./ID:339409Custom LNA Oligonucleotide Manual Design (5 µmol synthesis)Go to GeneGlobeCustom LNA Oligonucleotide Manual Design, designed by QIAGEN"s LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 5 µmolCat No./ID:339410Custom LNA Oligonucleotide Manual Design (10 µmol synthesis)Go to GeneGlobeCustom LNA Oligonucleotide Manual Design, designed by QIAGEN"s LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 10 µmolCat No./ID:339411Custom LNA Oligonucleotide (100 nmol synthesis)Go to GeneGlobe100 nmol synthesis Custom LNA Oligonucleotide; various purification and labeling options availableCat No./ID:339412Custom LNA Oligonucleotide (250 nmol synthesis)Go to GeneGlobe250 nmolsynthesis Custom LNA Oligonucleotide; various purification and labeling options availableCat No./ID:339413Custom LNA Oligonucleotide (1 µmol synthesis)Go to GeneGlobe1 µmol synthesis Custom LNA Oligonucleotide; various purification and labeling options availableCat No./ID:339414Custom LNA Oligonucleotide (5 µmol synthesis)Go to GeneGlobe5 µmolsynthesis Custom LNA Oligonucleotide; various purification and labeling options availableCat No./ID:339415Custom LNA Oligonucleotide Large Scale (A)Go to GeneGlobeCustom LNA Oligonucleotide Large Scale; various purification and labeling options; available in amounts between 5 mg – 1 kgCat No./ID:339416Custom LNA Oligonucleotide Large Scale (B)Go to GeneGlobeCustom LNA Oligonucleotide Large Scale; various purification and labeling options; available in amounts between 5 mg – 1 kgCat No./ID:339417Custom LNA Oligonucleotide (10 µmol synthesis)Go to GeneGlobe10 µmolsynthesis Custom LNA Oligonucleotide; various purification and labeling options availableCustom LNA Oligonucleotides are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.Product Details The power of Tm normalization.Signal intensity from microarray experiments using LNA-enhanced or DNA-based capture probes. miRNA targets with varying GC contentwere added at 100 amol each. The signal from DNA-based capture probes varies with GC content and results in poor detection of many miRNAs, whereas LNA probes offer robust detection of all miRNAs.Replace DNA with LNA for higher melting temperature.On the left, progressive substitutions of DNA nucleotides with LNA increases the melting temperature of the oligonucleotide, while maintaining the recognition sequence and specificity of the probe. On the right, LNA substitutions allow shortening of the probe, while maintaining the same Tm.PerformanceLNA oligonucleotides exhibit unprecedented thermal stability when hybridized to a complementary DNA or RNA strand. For each incorporated LNA monomer, the melting temperature (Tm) of the duplex increases by 2–8°C (see figure Replace DNA with LNA for higher melting temperature). In addition, LNA oligonucleotides can be made shorter than traditional DNA or RNA oligonucleotides and still retain a high Tm. This is important when the oligonucleotide is used to detect small or highly similar targets.Since LNA oligonucleotides typically consist of a mixture of LNA and DNA or RNA, it is possible to optimize the sensitivity and specificity by varying the LNA content of the oligonucleotide. Incorporation of LNA into oligonucleotides has been shown to improve sensitivity and specificity for many hybridization-based technologies including PCR, microarrays and in situ hybridization (ISH).Tm normalization enables robust detection, regardless of GC content. The Tm of a nucleotide duplex can be controlled by varying the LNA content. This feature can be used to normalize the Tm across a population of short sequences with varying GC content. For AT-rich nucleotides, which give low melting temperatures, more LNA is incorporated into the LNA oligonucleotide to raise the Tm of the duplex. This enables the design of LNA oligonucleotides with a narrow Tm range, which is beneficial in many research applications such as microarrays, PCR and other applications in which sensitive and specific binding to many different targets must occur under the same conditions simultaneously. The power of Tm normalization is demonstrated by the comparison of DNA and LNA probes for detection of miRNA targets with a range of CG content (see figure The power of Tm normalization).PrincipleUse the guidelines below when designing your own Custom LNA Oligonucleotides: LNA will bind very tightly to other LNA residues. Avoid self-complementarity and cross-hybridization to other LNA-containing oligonucleotides Keep the GC content between 30–60% Avoid stretches of more than 4 LNA bases, except when very short (9–10 nucleotides) oligonucleotides are designed Avoid stretches of 3 or more Gs or Cs For novel applications, design guidelines may have to be established empiricallyApplicationsLNA oligonucleotides can be successfully used in a wide range of applications, including: miRNA research Small RNA research SNP genotyping mRNA antisense oligonucleotides Allele-specific PCR RNAi DNAzymes Fluorescence polarization probes Molecular beacons Microarray gene expression profiling Gene repair/exon skipping Splice variant detection Comparative genome hybridization (CGH)Product ResourcesYou are not authorized to download the resourcefragment fix placeholderProduct citations in scientific articlesPowered by Bioz See more details on BiozCustomers who bought these products also boughtCat No./ID:13500-50RNeasy PowerPlant Kit (50)For isolating total RNA from difficult plant and seed samples$318.00Add To CartCat No./ID:204745 QuantiTect Multiplex PCR NoROX Kit (1000) For 1000 x 50 µl reactions: 25 ml 2x QuantiTect Multiplex PCR NoROX Master Mix (without ROX dye), 20 ml RNase-Free Water$2,983.00Add To CartCat No./ID:19086 Buffer EB (250 ml) 250 ml Elution Buffer$36.10Add To CartCat No./ID:1027415FlexiTube siRNA (1 nmol)1 nmol siRNA delivered in tubesSelect TargetsCat No./ID:60404QIAamp DSP DNA FFPE Tissue Kit (50)For 50 DNA preps: QIAamp MinElute columns, ProteinaseK, Buffers, and Collection Tubes (2ml)$269.00Add To CartCat No./ID:218073miScript SYBR Green PCR Kit (200)For 200 reactions: QuantiTect SYBR Green PCR Master Mix, miScript Universal Primer$478.00Add To CartCat No./ID:339317miRCURY LNA miRNA Custom PCR AssayContains forward and reverse primers for 200 reactions; for SYBR® Green-based detectionSelect TargetsCat No./ID:69106DNeasy Plant Mini Kit (250)250 DNeasy Mini Spin Columns, 250 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml)$1,083.00Add To CartCat No./ID:79306QIAzol Lysis Reagent (200ml)200 ml QIAzol Lysis Reagent$336.00Add To CartCat No./ID:79217Buffer EL (1000 ml)1000 ml Erythrocyte Lysis Buffer$184.00Add To CartCat No./ID:333074QIAseq UPX 3" Trans. 12-Index (48)For indexing up to 12 samples per sequencing lane on Illumina NGS instruments; multiple 96- or 384-well plates can be combined as one sample allowing for 12 x 96-well plates (1152 cells/wells) or 12 x 384-well plates (4608 cells/wells) per lane$1,033.00Add To CartCat No./ID:50214QIAamp UCP Pathogen Mini Kit50 QIAamp UCP Mini Columns, Collection Tubes (2 ml), Tube Extenders (20 ml), Elution Tubes, VacConnectors, Buffers, and Proteinase K$345.00Add To CartCat No./ID:79254RNase-Free DNase Set (50)1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps$113.00Add To CartCat No./ID:218300miScript Primer Assay (100)10x miScript Primer Assay (contains one miRNA-specific primer)Select TargetsCat No./ID:333088QIAseq UPX 3" Transcriptome Kit (96)For 3" gene expression analysis from cells or mRNA of 8–96 samples at one time; each kit contains cell lysis solution, buffers, oligos and enzymes lyophilized in a 96-well plate which can be easily separated for processing 8–96 samples in parallel (this format is ideal for both small and large sample sizes)$2,959.00Add To CartCat No./ID:339340miRCURY LNA RT KitFor 8–64 cDNA synthesis reactions: 5x RT SYBR Green Reaction Buffer, 5x RT Probe Reaction Buffer, 10x RT Enzyme Mix, UniSp6, RNA Spike-in template, RNase-Free Water$373.00Add To CartCat No./ID:218161miScript II RT Kit (50)For 50 cDNA synthesis reactions: miScript Reverse Transcriptase Mix, 10x miScript Nucleics Mix, 5x miScript HiSpec Buffer, 5x miScript HiFlex Buffer, RNase-Free Water$522.00Add To CartCat No./ID:181910GeneRead QIAact Actionable Insights Tumor PanelSets of 4 pools containing wet-bench verified primer sets for targeted enrichment of a pathway-focused panel of genes.Select TargetsCat No./ID:150345 REPLI-g Single Cell Kit (96) REPLI-g sc Polymerase, Buffers, and Reagents for 96 whole genome amplification reactions (yields up to 40 µg/reaction)$1,902.00Add To CartCat No./ID:339306miRCURY LNA miRNA PCR AssayContains forward and reverse primers for 200 reactions; for SYBR® Green-based detectionSelect Targets