Oncologyipsogen Leukemia28therascreen Solid Tumor9Circulating Tumor Cells6ipsogen BCR-ABL1 Mbcr IS-MMR KitsPrint For the quantification of BCR-ABL1 Mbcr b3a2 & b2a2 fusion gene transcripts and results alignment on the international scale Results on the international scale (IS) and MMR reporting One IS-MMR calibrator per run to prevent drifting High reliability using EAC technology and single plasmid standards Optimal quality control and ISO 13485 certification Easy workflow with ready-to-use solutions ipsogen BCR-ABL1 Mbcr IS-MMR Kits are intended for research use only and are not for use in diagnostic procedures. The kits are for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kits provide reagents optimized for reliable and sensitive detection and quantitation of BCR-ABL p210 b2a2 or b3a2 transcripts, relative to ABL control gene expression.Buy ProductsProduct DetailsProduct ResourcesBuy ProductsCat No./ID:670713ipsogen BCR-ABL1 Mbcr IS-MMR Kit (24)Log in to see pricingFor 24 reactions: High Positive RNA Control, IS-MMR Calibrator, Mbcr and ABL Single Plasmid Standards, Primers and Probe Mix ABL, Primers and Probe Mix BCR-ABL Mbcr Fusion GeneCat No./ID:670813ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit (24)Log in to see pricingFor 24 reactions: Reverse transcriptase, 5x RT buffer, dNTP mix, Random primers, RNase Inhibitor, DTT, High Positive RNA Control, IS-MMR Calibrator, Mbcr and ABL Single Plasmid Standards, Primers and Probe Mix ABL, Primers and Probe Mix BCR-ABL Mbcr Fusion Gene, qPCR Master mix, ROX II fluorescent dyeFor Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.Product Details Reliable detection of ABL plasmid standards, high positive RNA control, and IS-MMR calibrator.Accurate detection of Mbcr plasmid standards, high positive RNA control, and IS-MMR calibrator.ABL control gene transcript.Single plasmid for BCR-ABL gene transcript.PerformanceTo ensure highest sensitivity, ipsogen BCR-ABL1 Mbcr IS-MMR Kits have been optimized to detect low levels of BCR-ABL p210 b2a2 or b3a2 transcripts (see figuresAccurate detection of Mbcr plasmid standards, high positive RNA control, and IS-MMR calibrator and Reliable detection of ABL plasmid standards, high positive RNA control, and IS-MMR calibrator). The sensitivity has a limit of detection (LOD) of 0.0069.Principleipsogen BCR-ABL1 Mbcr IS-MMR Kits are ready-to-use kits for the detection of BCR-ABL p210 b2a2 or b3a2 transcripts using real-time PCR. The kits are based on the amplification and detection of specific BCR-ABL p210 b2a2 or b3a2 transcripts, relative to ABL control gene expression, in total RNA. The kits provide high levels of specificity, sensitivity, and reproducibility. Each ipsogen BCR-ABL1 Mbcr IS-MMR Kit provides 4 standard dilutions for ABL and 5 standard dilutions for Mbcr. Use of a single plasmid for BCR-ABL1 and ABL enables reproducible quantification of transcripts and limits variability. Primers and probe sets are designed according to Europe Against Cancer (EAC) recommendations (see figuresSingle plasmid for BCR-ABL gene transcript and ABL control gene transcript). The kits also include an IS-MMR calibrator allowing conversion of results to the international scale for accurate measurement of molecular response and major molecular response (MMR) status reporting. A high positive control RNA is also included to monitor the reverse transcription and amplification steps of ABL and BCR-ABL Mbcr during transcript quantification. The ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit includes reagents for reverse transcription-PCR and real-time PCR. ProcedureThe first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using ipsogen BCR-ABL1 Mbcr IS-MMR Kits allows detection and quantification of BCR-ABL p210 b2a2 or b3a2 and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.ApplicationsThe ipsogen BCR-ABL1 Mbcr IS-MMR Kits enable sensitive and reliable detection and quantification of BCR-ABL p210 b2a2 or b3a2 transcripts, conversion of results to the international scale, and MMR reporting, for research use only.Product ResourcesYou are not authorized to download the resourceFAQs (1) Sort optionsSort alphabetically ViewShow allShow lessKit Handbooks (2) Sort optionsSort alphabetically ipsogen BCR-ABL1 Mbcr IS-MMR HandbookShow detailsipsogen BCR-ABL1 Mbcr IS-MMR DX HandbookShow detailsShow allShow lessSafety Data Sheets (2) Sort optionsSort alphabetically MSDS ipsogen BCR-ABL1 Mbcr IS-MMR Kit (24)MSDS ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit (24)Show allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:61104QIAamp DSP DNA Blood Mini KitFor 50 preps: QIAamp Mini Spin Columns, Buffers, Reagents, Tubes, VacConnectors$175.00Add To CartCat No./ID:52304QIAamp RNA Blood Mini Kit (50)For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free reagents and buffers$385.00Add To CartCat No./ID:79217Buffer EL (1000 ml)1000 ml Erythrocyte Lysis Buffer$184.00Add To CartCat No./ID:981103Strip Tubes and Caps, 0.1 ml (250)250 strips of 4 tubes and caps for 1000 reactions of 10–50 µl$250.00Add To CartCat No./ID:764133PAXgene Bone Marrow RNA Kit (30)For 30 RNA preps: 30 PAXgene Spin Columns, 30PAXgene Shredder Spin Columns, Processing Tubes, RNase-free DNase I, RNase-free reagents and buffers. 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