OverviewHarness AAV technology with SBI’s AAVanced rAAV Vectors Widely used for gene therapy development and gene editing in vivo because of their broad tropism, lack of associated disease, the ability to transduce both dividing and non-dividing cells, and long-term transgene expression, recombinant AAV vectors are becoming increasingly popular. To help researchers take advantage of the powerful AAV system, SBI has developed a series of AAV vectors optimized for easy cloning and high titers. The pAAVK-EF1α-MCS-T2A-mRFP AAVanced™ Cloning and Expression Vector is an AAV vector that enables co-expression of a gene-of-interest and the mRFP reporter by the EF1α promoter.Because packaging into the AAV capsid limits the size of AAV vectors, the total amount of DNA between the two ITRs in SBI’s AAVanced Vectors needs to be 5 kb or less.Choose the right AAVanced Vector for your projectSBI’s family of AAVanced Cloning and Expression Vectors support a range of projects:Optimized for high titer rAAV productionAvailable in single and dual promoter formatsCompatible with any AAV packaging systemDesigned for easy & efficient cloningChoose fluorescent or antibiotic selection markersCat.#VectorMarkerFeaturesAAV502A-1pAAVK-EF1α-MCSNoneStreamlined sizeAAV503A-1pAAVK-EF1α-MCS1-CMV-MCS2NoneFlexible—dual promoter, dual MCSAAV526A-1pAAVK-EF1α-MCS-T2A-EGFPEGFPCo-expression with markerAAV527A-1pAAVK-EF1α-MCS-T2A-PuroPuroCo-expression with markerAAV528A-1pAAVK-EF1α–MCS-T2A-mRFPmRFPCo-expression with markerAAV536A-1pAAVK-EF1α-MCS1-CMV-EGFPEGFPDual promoter with markerAAV537A-1pAAVK-EF1α-MCS1-CMV-PuroPuroDual promoter with markerAAV538A-1pAAVK-EF1α-MCS1-CMV-mRFPmRFPDual promoter with markerSupporting DataEffective gene delivery and expression with AAVanced Cloning and Expression VectorsFigure 1. SBI’s single promoter AAVanced Cloning and Expression vectors are delivered effectively to target cells and express the desired markers. Representative images for GFP/RFP/Puro marker expression in HT1080 cells transduced with pAAVK-EF1α-MCS-T2A-EGFP (top row), pAAVK-EF1α-MCS-T2A-mRFP (middle row), or pAAVK-EF1α-MCS-T2A-Puro (bottom row). The top two rows compare cells transduced with the indicated vector packaged into AAV particles versus cells transfected with the same vector. Both conditions show good expression of GFP or RFP. The bottom row shows the number of cells present after three days of puromycin selection in the well with or without infection with pAAVK-EF1-MCS-2A-Puro packaged into AAV particles.ResourcesUser Manual: AAVanced AAV VectorsBrochure: Gene Delivery and Expression Products and Services Related productspCDH-CMV-MCS-EF1α-copGFP Cloning and Expression LentivectorShop NowpCDH-CMV-MCS-T2A-copGFP Cloning and Expression LentivectorShop NowpAAVK-EF1α-MCS-CMV-Puro AAVanced™ Cloning and Expression VectorShop NowpCDH-CMV-MCS-EF1α-GreenPuro Cloning and Expression LentivectorShop NowpAAVK-EF1α-MCS1-CMV-MCS2 AAVanced™ Cloning and Expression VectorShop NowpCDH-EF1α-MCS Cloning and Expression LentivectorShop Now

美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1