OverviewGet your miRNA ready for qPCR profilingWhen you’d like to profile miRNA with qPCR, SBI’s QuantiMir™ Kit can get your samples ready. By efficiently converting all of your miRNA into cDNA, you can get accurate, sensitive, and quantitative qPCR data on the miRs in your sample. Great for understanding differential miRNA expression, simultaneously profiling siRNA and mRNA, and more.Simple and robust procedureRapidly and efficiently convert all small RNAs into cDNAs for qPCRSuitable for high-throughput screening of clinical samplesSensitive and accurate (see the data in the Supporting Data section below)Versatile—design your own miRNA assaysEach kit includes three reference assays—human U6, mouse U6 and human/mouse miR-16. We also offer the following reference assays as individual 50-assay kits:Catalog #Amplified small RNASpecies compatibilityRA420A-hU6Human U6HumanRA420A-miR-16miR-16Human, MouseRA420A-mU6Mouse U6MouseRA420A-RNU1RNU1 (U1)Human, Mouse, Rat, MonkeyRA420A-RNU43RNU43Human, Mouse, RatRA420A-rU6Rat U6RatHow It WorksThe QuantiMir Kit enables robust miRNA quantitation through a simple and efficient workflowHighly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR—profile thousands of different miRNAs from a single reverse transcription reaction.Tag all small RNAs with a poly-A tailAnneal an oligo-dT adaptorReverse transcribe to create first-strand cDNAThe result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.Create custom miRNA assaysTo design your own miRNA assays, simply synthesize an oligo using the sequence of the mature miRNA you’d like to profile as the forward primer in your miRNA qPCR assay, and use with the universal reverse primer included in the QuantiMir Kit.Supporting DataConverting miRNA into cDNA for accurate qPCR profiling and quantitationThe QuantiMir Kit is sensitiveFigure 1. The QuantiMir Kit is highly sensitive and enables measurement across a wide dynamic range. (A) Starting with total, Trizol-extracted RNA or fractionated small RNA samples you can measure from several micrograms down to picograms of input RNA with excellent accuracy. (B) You can also detect differential miRNA expression across a dynamic range of at least 6 logs.The QuantiMir Kit is accurateFigure 2. The QuantiMir Kit is accurate. We used the QuantiMir Kit to synthesize first strand cDNAs from 18 different human tissues: adipose, bladder, brain, cervix, colon, esophagus, heart, kidney, liver, lung, ovary, placenta, prostate, skeletal muscle, small intestine, spleen, testes, and thymus. The cDNAs were balanced to yield equal Ct values for the U6 snRNA normalizing transcript (bottom left plot, green bars). Real-time PCR results demonstrate that the normalizing snRNA is uniformly expressed across the 18 tissues examined. As expected, assays for miR-1 are show specific expression in heart and musculoskeletal tissues (top left plot, red bars) whereas assays for miR-122 show specific expression in the liver (top right plot, blue bar).Use the QuantiMir Kit to profile miRNAs from cancerous and normal tissuesFigure 3. Use the QuantiMir Kit to profile miRNAs from cancerous and normal tissues. Example of quantitative miRNA profiling of nine miRNAs in five different normal and tumor-derived samples.Measure both siRNA and mRNA knockdown in a single QuantiMir cDNA sampleFigure 4. Measure both siRNA and mRNA knockdown in a single QuantiMir cDNA sample. A time-course using anti-p53 shRNA-directed knockdown of the endogenous p53 mRNA transcript demonstrates how the QuantiMir Kit can be used to measure both p53 siRNA (orange bars) and p53 mRNA (blue line) via qPCR.ResourcesUser Manual: QuantiMir KitProtocol: Cells to CtsApplication Note: QuantiMir KitApplication Note: QuantiMir Dissociation AnalysisNote: QuantiMir Control Assay InformationProduct Sheet: QuantiMir KitPresentation Slides: QuantiMir miRNA ApplicationsPresentation Slides: Introduction to QuantiMirCitationsBhome, R, et al. (2017) Profiling the MicroRNA Payload of Exosomes Derived from Ex Vivo Primary Colorectal Fibroblasts. Methods Mol. Biol..2017 Nov 9; 1509:115-122. PM ID:27826922Dluzen, DF, et al. (2017) MicroRNAs Modulate Oxidative Stress in Hypertension through PARP-1 Regulation. Oxid Med Cell Longev.2017 Jun 29; 2017:3984280. PM ID:28660007Su, YK, et al. (2017) Antrodia cinnamomea sensitizes radio-/chemo-therapy of cancer stem-like cells by modulating microRNA expression. J Ethnopharmacol.2017 Jun 8;. PM ID:28602756Min, KW, et al. (2017) AUF1 facilitates microRNA-mediated gene silencing. Nucleic Acids Res..2017 Jun 2; 45(10):6064-6073. PM ID:28334781Zheng, H, et al. (2017) Alcohol-dysregulated microRNAs in hepatitis B virus-related hepatocellular carcinoma. PLoS ONE.2017 May 31; 12(5):e0178547. PM ID:28562643Paul, D, et al. (2017) A-to-I editing in human miRNAs is enriched in seed sequence, influenced by sequence contexts and significantly hypoedited in glioblastoma multiforme. Sci Rep.2017 May 26; 7(1):2466. PM ID:28550310Reeves, ME, et al. (2017) Identification and characterization of RASSF1C piRNA target genes in lung cancer cells. Oncotarget.2017 May 23; 8(21):34268-34282. PM ID:28423657Brodie, S, et al. (2017) The novel long non-coding RNA TALNEC2, regulates tumor cell growth and the stemness and radiation response of glioma stem cells. Oncotarget.2017 May 9; 8(19):31785-31801. PM ID:28423669Gao, S, et al. (2017) Identification and characterization of miRNAs in two closely related C4 and C3 species of Cleome by high-throughput sequencing. Sci Rep.2017 Apr 19; 7:46552. PM ID:28422166Yang, Y, Bai, YS & Wang, Q. (2017) CDGSH Iron Sulfur Domain 2 Activates Proliferation and EMT of Pancreatic Cancer Cells via Wnt/β-Catenin Pathway and Has Prognostic Value in Human Pancreatic Cancer. Oncol. Res..2017 Apr 14; 25(4):605-615. PM ID:27983920Tsai, HC, et al. (2017) WISP-1 positively regulates angiogenesis by controlling VEGF-A expression in human osteosarcoma. Cell Death Dis.2017 Apr 13; 8(4):e2750. PM ID:28406476Ramachandran, M, et al. (2017) Safe and Effective Treatment of Experimental Neuroblastoma and Glioblastoma Using Systemically Delivered Triple MicroRNA-Detargeted Oncolytic Semliki Forest Virus. Clin. Cancer Res..2017 Mar 15; 23(6):1519-1530. PM ID:27637889Fafián-Labora, J, et al. (2017) Effect of age on pro-inflammatory miRNAs contained in mesenchymal stem cell-derived extracellular vesicles. Sci Rep.2017 Mar 6; 7:43923. PM ID:28262816Maeda, Y, et al. (2017) Synovium-Derived MicroRNAs Regulate Bone Pathways in Rheumatoid Arthritis. J. Bone Miner. Res..2017 Mar 1; 32(3):461-472. PM ID:27676131Chen, S, et al. (2017) H19 Overexpression Induces Resistance to 1,25(OH)2D3 by Targeting VDR Through miR-675-5p in Colon Cancer Cells. Neoplasia.2017 Mar 1; 19(3):226-236. PM ID:28189050Hou, S, et al. (2017) MicroRNA-939 governs vascular integrity and angiogenesis through targeting γ-catenin in endothelial cells. Biochem. Biophys. Res. Commun..2017 Feb 26; 484(1):27-33. PM ID:28115160Yeung, CL, et al. (2017) Human papillomavirus type 16 E6 suppresses microRNA-23b expression in human cervical cancer cells through DNA methylation of the host gene C9orf3. Oncotarget.2017 Feb 14; 8(7):12158-12173. PM ID:28077801Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine.2017 Feb 1; 15:163-172. PM ID:27974246Monsanto-Hearne, V, et al. (2017) Drosophila miR-956 suppression modulates Ectoderm-expressed 4 and inhibits viral replication. Virology.2017 Feb 1; 502:20-27. PM ID:27960110Small, RNA. (2017) QuantiMir RT Kit Small RNA Quantitation System. Product.;. Link:ProductSearch all Citations Related productsQuantiMir™ Rat U6 Reference AssaysShop NowPooled Lenti-miR™ Virus LibraryShop NowQuantiMir™ miR-16 Reference AssaysShop NowQuantiMir™ RNU43 Reference AssaysShop NowmiRZip™ anti-miRNA Expression LentivectorsShop NowOncoMir qPCR ArrayShop Now

美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

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ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1