OverviewProfile across the mouse miRNomeSBI has simplified qPCR profiling of mouse miRNAs by bringing together a comprehensive qPCR array of mouse miRNAs. Our Mouse mmu-miRNome MicroRNA Profiling Kit comes with all the reagents necessary to tag and convert small RNAs into quantifiable cDNA using the sensitive QuantiMir™ technology and a large set of mouse miRNA assays, including major and minor miR forms (709 mature miRNAs).The kits include assays in two, pre-formatted 384-well assay plates with three endogenous reference miRNA controls on each plate (U1, U6, and RNU43). All microRNA assays are based on miRBase entries.Identify miRNA biomarkers and expression pattern signaturesRapidly tag and convert all small RNAs into detectable cDNA for qPCRMeasure as little as picogram amounts of starting total RNAConduct high-throughput screens of cell lines, tissues, and clinical samplesAbout the included QuantiMir KitWhen you’d like to profile miRNAs with qPCR, SBI’s QuantiMir™ Kit can get your samples ready. By efficiently converting all of your miRNA into cDNA, you can get accurate, sensitive, and quantitative qPCR data on the miRs in your sample. Great for understanding differential miRNA expression, simultaneously profiling siRNA and mRNA, and more.Simple and robust procedureRapidly and efficiently convert all small RNAs into cDNAs for qPCRSuitable for high-throughput screening of clinical samplesSensitive and accurateVersatile—design your own miRNA assaysHow It WorksUsing the mmu-miRNome MicroRNA Profiling KitFirst, prepare RNA for qPCR using the QuantiMir Kit Highly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR—profile thousands of different miRNAs from a single reverse transcription reaction.Tag all small RNAs with a poly-A tailAnneal an oligo-dT adaptorReverse transcribe to create first-strand cDNAThe result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.Then conduct qPCR assaysGet additional information about the array including miRNA sequences, miRbase accession numbers, and qPCR analysis templates in the Excel document, “Free Analysis Software for Mouse miRNome Ver. 14”Supporting DatamiRNome Profiler Performance DataFigure 1. Accurately normalize your microRNA expression profiling data using three reference controls—U6 snRNA, RNU1A, and RNU43.Figure 2. Get high reproducibility—excellent technical replicates across 8-logs. Reliable replicates from plate-to-plate enable precise comparisons of numerous samples across large microRNA profiling studies.ResourcesUser Manual: miRNome miRNA ProfilersProduct Sheet: miRNome miRNA ProfilerCitationsOlivieri, F, et al. (2016) Circulating miRNAs and miRNA shuttles as biomarkers: Perspective trajectories of healthy and unhealthy aging. Mech. Ageing Dev..2016 Dec 13;. PM ID:27986629Khamaisi, M, et al. (2016) PKCδ inhibition normalizes the wound-healing capacity of diabetic human fibroblasts. J. Clin. Invest..2016 Mar 1; 126(3):837-53. PM ID:26808499Pandey, S, et al. (2015) The RNA Stem-Loop to G-Quadruplex Equilibrium Controls Mature MicroRNA Production inside the Cell. Biochemistry.2015 Dec 8; 54(48):7067-78. PM ID:26554903Meiler, S, et al. (2015) MicroRNA 302a is a novel modulator of cholesterol homeostasis and atherosclerosis. Arterioscler. Thromb. Vasc. Biol..2015 Feb 1; 35(2):323-31. PM ID:25524771岡本行広, , et al. (2015) マイクロ抽出場によるヒト腫瘍細胞由来のマイクロ RNA 抽出法. 分析化学.2015 Jan 31; 64(1):9-13. Link:分析化学Kumarswamy, R, et al. (2012) SERCA2a gene therapy restores microRNA-1 expression in heart failure via an Akt/FoxO3A-dependent pathway. Eur. Heart J..2012 May 1; 33(9):1067-75. PM ID:22362515Alpini, G, et al. (2011) Regulation of placenta growth factor by microRNA-125b in hepatocellular cancer. J. Hepatol..2011 Dec 1; 55(6):1339-45. PM ID:21703189Crother, TR, et al. (2011) Chlamydia pneumoniae infection induced allergic airway sensitization is controlled by regulatory T-cells and plasmacytoid dendritic cells. PLoS ONE.2011 Jun 22; 6(6):e20784. PM ID:21695198Stellato, C, et al. (2011) Glucocorticoid (GC) Modulation of Global miRNA Profile in Human Airway Epithelial Cells. Journal of Allergy and Clinical Immunology.2011 Feb 1; 127(2S):AB64. Link:Journal of Allergy and Clinical ImmunologyKarim, Z, et al. (2011) Signaling Mechanism of Tolerance following Repetitive Stimulation with a Single Allergen and its Reversal by Stimulation with Multiple Allergens. Journal of Allergy and Clinical Immunology.;:AB64. Link:Journal of Allergy and Clinical ImmunologySearch all Citations Related productsStem Cell miRNA Profiling PanelShop Nowhsa-miRNome MicroRNA Profiling Kit (Human)Shop NowOncoMir qPCR ArrayShop NowmiRZip™ anti-miRNA Expression LentivectorsShop Nowrno-miRNome MicroRNA Profiling Kit (Rat)Shop NowQuantiMir™ KitShop Now

美国SBI代理

System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

美国SBI代理

ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1