OverviewMonitor neurogenesis in real timeWith SBI’s line of pGreenZeo, pRedZeo, and pRedTK Differentation Reporter Vectors, you can monitor stem cell differentiation in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built differentiation reporters come as ready-to-package lentivector plasmids or ready-to-transduce pre-packaged lentivirus. The Human CD11b pGreenZeo Differentiation Reporter co-expresses dscGFP (destabilized copGFP, 2-hour half-life) and zeomycin resistance from the human CD11b promoter/enhancer elements, enabling visualization of neurogenesis using GFP fluorescence and selection for the desired cells using zeomycin.Create stable neurogenesis-reporting cell linesMonitor multiple lineages simultaneouslyTrack differentiation in live cells in real timePlease note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.Choose the differentiation reporter that’s right for youNeural differentiation reportersCatalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional namesSR1008PA/VA-1Macrophage, microgliaMouseCd68Cd68macrosialin, Scard1, gp110SR10015PA/VA-1AstrocyteHumanGFAPGFAPGlial fibrillary acidic proteinSR10016PA/VA-1AstrocyteMouseGfapGfapGlial fibrillary acidic proteinSR10017PA/VA-1MicrogliaHumanCD11bITGAMintegrin, alpha M, CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1ASR10018PA/VA-1MicrogliaMouseEMR1Emr1EGF-like module containing, mucin-like, hormone receptor-like sequence 1, EGF-TM7, F4/80SR10019PA/VA-1MicrogliaMouseIba-1Aif1allograft inflammatory factor 1 (Aif-1), ionized calcium binding adapter molecule 1 (Iba-1)SR10020PA/VA-1Muller gliaMouseCd44Cd44HERMES, Ly-24, Pgp-1SR10021PA/VA-1NeuronHumanBM88Cend1cell cycle exit and neuronal differentiation 1 (Cend1)SR10022PA/VA-1NeuronMouseCamk2aCamk2aCaMK II; alpha-CaMKIISR10023PA/VA-1NeuronMouseGAD67Gad1glutamic acid decarboxylase 1, EP10, GAD67, Gad-1SR10024PA/VA-1NeuronRatNSEEno2enolase 2, gamma (Eno2), neuron-specific enolase (NSE)SR10025PA/VA-1NeuronMouseTa1 a-tubulinTuba1atubulin, alpha 1A (Tuba1a), Tuba-1, Tuba1SR10026PA/VA-1OligodendrocyteMouseMBPMbpmyelin basic protein (Mbp), myelin deficient; shivererSR10027PA/VA-1PhotoreceptorHumanOpsinOPN1SWopsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan)SR10034PA/VA-1Neural Stem CellRatNestinNesNesSR10035PA/VA-1Neural Stem CellHumanNestinNESNESSR10041PA/VA-1NeuronHumanDoublecortinDCXDBCN, DC, LISX, SCLH, XLISSR10047PA/VA-1NeuronHumanMAP2MAP2MAP2A, MAP2B, MAP2CSR10048PA/VA-1NeuronHumanFABP7FABP7B-FABP, BLBP, DKFZp547J2313, FABPB, MRGMyogenic differentiation reportersCatalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional namesSR10010PA/VA-1CardiomyocyteMouseActcActc1Cardiac muscle alpha actin 1SR10011PA/VA-1CardiomyocyteHumanMLC-2vMYL2MYL2, MLC2SR10012PA/VA-1CardiomyocyteHumanTNNT2TNNT2Troponin T type 2 (cardiac), TnTC, cTnTSR10013PA/VA-1CardiomyocyteMouseTnnt2Tnnt2Tnt, cTnTSR10014PA/VA-1Smooth muscle myocyteMouseSM22aTaglnTransgelin (Tagln), SM22, Sm22aSR10049PA/VA-1CardiomyocyteHumanACTCACTC1ACTC, CMD1RSR10050PA/VA-1Skeletal myocyteMouseMyogeninMyogmyo; MYF4Hematopoietic differentiation reportersCatalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional namesSR1004PA/VA-1B-cellHumanB29CD79BCD79b, Ig-beta, Igb, IgbetaSR1005PA/VA-1B-cellMouseB29Cd79bCd79bSR1006PA/VA-1CD8 T-cellMouseCD8Cd8aBB154331, Ly-2, Ly-35, Ly-B, Lyt-2SR1007PA/VA-1ErythroidHumanHLA-DRaHLA-DRAHLA-DRA1, major histocompatibility complex, class II, DR alphaSR1008PA/VA-1Macrophage, microgliaMouseCd68Cd68macrosialin, Scard1, gp110SR1009PA/VA-1Pan T-cellHumanCD2CD2SRBC, T11SR10032PA/VA-1LymphocyteHumanLCKLCKYT16, p56lck, pp58lckStructural differentiation reportersCatalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional namesSR1001PA/VA-1ChondrocyteMouseCol2a1Col2a1collagen, type II, alpha 1SR1002PA/VA-1OsteoblastHumanSPP1SPP1secreted phosphoprotein 1 (SPP1), osteopontin (OPN)SR1003PA/VA-1OsteoblastHumanOsteocalcinBGLAPbone gamma-carboxyglutamate (BGLAP), BGP, PMF1SR10036PA/VA-1AdipocyteMouseALBPFabp4422/aP2, ALBP/Ap2, Ap2, LbplSR10038PA/VA-1EpitheliumHumanKeratin 14KRT14CK14, EBS3, EBS4, K14, NFJSignaling differentiation reportersCatalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional namesSR10028PA/VA-1Beta cellHumanInsulinINSinsulinSR10037PA/VA-1IsletHumanNGN3NGN3neurogenin 3, Atoh5, Math4B, bHLHa7, neurog3SR10039PA/VA-1IsletHumanPDX1PDX1IDX-1, IPF1, IUF1, MODY4, PDX-1, STF-1SR10040PA/VA-1IsletMousePdx1Pdx1IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1Supporting DataSee some of our differentiation reporters in actionSBI’s differentiation reporters are used in a number of papers. The data shown below are just one example (from Ravin R, Hoeppner DJ, Munno DM, Carmel L, Sullivan J, Levitt DL, Miller JL, Athaide C, Panchision DM, McKay RD. Potency and fate specification in CNS stem cell populations in vitro. Cell Stem Cell. 2008 Dec 4; 3(6):670-80. PMID: 19041783)Figure 1. Live imaging of neuronal differentiation. Ravin, et al, used SBI’s Human CD11b pGreenFire Differentiation Reporter (Cat.# SR10016VA-1), which drives GFP expression from the glial fibrillary acidic protein promoter, to watch human neural stem cells differentiate into a network of mature neurons, oligodendrocytes, and astrocytes over the course of seven days. The periodic “flashes” seen in this video correspond to fluorescent photos taken of the growing cells to identify the GFP signals. The final photo taken after the network formation is shown below the video (color added). Among the network of neurons, only the astrocytes are bright green, demonstrating the specificity of SBI’s human CD11b pGreenFire Differentiation Reporter.Figure 2. Simultaneously track multiple lineages from iPS and progenitor cells.Figure 3. Additional differentiation reporter data.ResourcesUser Manual: pGreenZeo (pRedZeo, pRedTK) Differentiation Reporter LentivectorsLentivirus Safety GuidelinesProduct Sheet: Reporters for Stem Cell ResearchPresentation Slides: Stem Cell Tools from SBI Related productsPlasma Membrane Cyto-Tracer™, pCT-Mem-GFP (MSCV)Shop NowMSCV-RFP-T2A-Luciferase Minicircle for In Vivo ImagingShop NowDual-Color Alkaline Phophatase Staining KitShop NowEF1α-RFP-T2A-Luciferase Minicircle for In Vivo ImagingShop NowInner Leaflet of the Plasma Membrane Cyto-Tracer™, pCT-InnerMem-GFP (MSCV)Shop NowMSCV-Luciferase-RFP-TK Lentivector for In Vivo ImagingShop Now

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System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

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ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1