OverviewLearn more about lncRNAs Profile 90 human lncRNAs with this kit that includes reagents for converting lncRNAs to cDNAs and a primer set for qPCR assays in a 96-well format. Get robust cDNA yields and clean, sensitive qPCR quantitation with undetectable background.The kit includes five positive housekeeping reference controls and one negative control. In addition, the kit supports subcellular localization studies through RNA reference controls for the nucleolus, nucleus, and cytoplasm:Nucleolus—snoRNA RNU43 (and some 18S rRNA)Nucleus—small Nuclear splicing snRNA U6BCytoplasm—GAPDH, Lamin A/C (Human Array) and Beta Actin (Mouse Array) as well as 18S rRNAHow It WorksUsing the LncRNA Profiler qPCR Array KitFirst, get your lncRNA ready for qPCR by converting to cDNA While some lncRNAs have endogenouse polyA tails, other lncRNAs do not. To enhance qPCR assay performance, the cDNA synthesis workflow includes steps and reagents to polyadenylate all lncRNAs before cDNA conversion using the tagged oligo dT adaptor and random primers. This combined RNA tailing and oligo dT plus random primers boosts cDNA yield significantly and enables strand-specific lncRNA qPCR profiling.Tag all lncRNAs with a poly-A tailAnneal an oligo-dT adaptorReverse transcribe to create first-strand cDNAThe result is a cDNA pool of anchor-tailed lncRNAs that are ready for qPCR.Then conduct qPCR assaysGet additional information about the array including lncRNA sequences, LncRNA database links, and qPCR analysis templates in the Excel document, “Assay List and Free Analysis Software for Human LncProfiler qPCR Array ”Supporting DataExample LncRNA Profiler qPCR Array Kit (Human) DataFigure 1. Sample dissociation analysis curves.Figure 2. Compare lncRNA expression in normal human fibroblasts and fibrosarcoma cells. LncRNAs known to be upregulated in cancer, such as Neat1, Anril, Hotair and Malat1, were all detected at highly elevated levels in the fibrosarcoma cells (HT1080) when compared to the normal fibroblast cells (HFF). The data are plotted as normalized expression levels.ResourcesUser Manual: lncRNA ProfilersProduct Sheet: lncRNA Profiler qPCR ArraysPoster: lncRNAsCitationsZhang, D, et al. (2017) Long noncoding RNA FOXD3-AS1 regulates oxidative stress-induced apoptosis via sponging microRNA-150. FASEB J..2017 Jun 27;. PM ID:28655711Bolha, L, Ravnik-Glavač, M & Glavač, D. (2017) Long Noncoding RNAs as Biomarkers in Cancer. Disease Markers.2017 May 29; 2017. Link:Disease MarkersGallo, A, et al. (2017) Global profiling of viral and cellular non-coding RNAs in Epstein-Barr virus-induced lymphoblastoid cell lines and released exosome cargos. Cancer Lett..2017 Mar 1; 388:334-343. PM ID:27956246Zhang, J, et al. (2017) Curcumin suppresses cisplatin resistance development partly via modulating extracellular vesicle-mediated transfer of MEG3 and miR-214 in ovarian cancer. Cancer Chemother. Pharmacol..2017 Mar 1; 79(3):479-487. PM ID:28175963Parasramka, M, et al. (2017) BAP1 dependent expression of long non-coding RNA NEAT-1 contributes to sensitivity to gemcitabine in cholangiocarcinoma. Mol. Cancer.2017 Jan 25; 16(1):22. PM ID:28122578Ma, L, et al. (2017) Long non-coding RNA XIST promotes cell growth and invasion through regulating miR-497/MACC1 axis in gastric cancer. Oncotarget.2017 Jan 17; 8(3):4125-4135. PM ID:27911852Zhang, Q, et al. (2016) Analysis of long noncoding RNA expression in hepatocellular carcinoma of different viral etiology. J Transl Med.2016 Nov 28; 14(1):328. PM ID:27894309Greco, S, et al. (2016) Long noncoding RNA dysregulation in ischemic heart failure. J Transl Med.2016 Jun 18; 14(1):183. PM ID:27317124Villamizar, O, et al. (2016) Data in support of transcriptional regulation and function of Fas-antisense long noncoding RNA during human erythropoiesis. Data Brief.2016 Jun 1; 7:1288-95. PM ID:27141526Qi, P, Zhou, XY & Du, X. (2016) Circulating long non-coding RNAs in cancer: current status and future perspectives. Mol. Cancer.2016 May 17; 15(1):39. PM ID:27189224Villamizar, O, et al. (2016) Data in Brief. Data in Brief.2016 Apr 7; 7:1288–1295. Link:Data in BriefVillamizar, O, et al. (2016) Fas-antisense long noncoding RNA is differentially expressed during maturation of human erythrocytes and confers resistance to Fas-mediated cell death. Blood Cells, Molecules, and Diseases.2016 Mar 3; 58:57-66. Link:Blood Cells, Molecules, and DiseasesKang, Y, et al. (2016) PCGEM1 stimulates proliferation of osteoarthritic synoviocytes by acting as a sponge for miR-770. J. Orthop. Res..2016 Mar 1; 34(3):412-8. PM ID:26340084Thorenoor, N, et al. (2016) Long non-coding RNA ZFAS1 interacts with CDK1 and is involved in p53-dependent cell cycle control and apoptosis in colorectal cancer. Oncotarget.2016 Jan 5; 7(1):622-37. PM ID:26506418Patel, K, et al. (2016) LncRNA regulation in mouse lung tumor from dibenzo [def, p] chrysene induced carcinogenesis in a transplacental Nrf2 knockout mouse model. Thesis.;. Link:ThesisCarter, G, et al. (2015) Circulating long noncoding RNA GAS5 levels are correlated to prevalence of type 2 diabetes mellitus. BBA Clin.2015 Dec 1; 4:102-7. PM ID:26675493Chen, S, et al. (2015) Macrophage infiltration promotes invasiveness of breast cancer cells via activating long non-coding RNA UCA1. Int J Clin Exp Pathol.2015 Oct 14; 8(8):9052-61. PM ID:26464647Shen, J, et al. (2015) Exploration of Deregulated Long Non-Coding RNAs in Association with Hepatocarcinogenesis and Survival. Cancers (Basel).2015 Sep 10; 7(3):1847-62. PM ID:26378581Shen, J, et al. (2015) Deregulated long non-coding RNAs in hepatocellular carcinoma (HCC). Cancer Research.2015 Aug 1; 75(15S):3818. Link:Cancer ResearchLin, WC, et al. (2015) Inhibitory effects of high stability fucoxanthin on palmitic acid-induced lipid accumulation in human adipose-derived stem cells through modulation of long non-coding RNA. Food Funct.2015 Jul 1; 6(7):2215-23. PM ID:26057608Search all Citations Related productsOncoMir Pooled miRNA Precursor Virus LibraryShop NowCells-to-Cts plus QuantiMir™ KitShop NowQuantiMir™ Rat U6 Reference AssaysShop NowmiRZip™ anti-miRNA Expression LentivectorsShop NowRegulatory RNA qPCR ProfilerShop NowRNA-Quant™ cDNA Synthesis KitShop Now

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System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒

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ExoQuick and ExoQuick-TC Products

Product	Size	Catalog #
ExoQuick serum exosome precipitation solution (5 ml)	75 reactions	EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product	75 reactions	EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product	100 reactions	TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml)	300 reactions	EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml)	10 reactions	EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml)	50 reactions	EXOTC50A-1
Exosome-depleted FBS Media Supplement - USA Certified	50 ml	EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement - USA Certified	250 ml	EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit	5 reactions	EXOLP5A-1