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Biotech Support Group(BSG)/HemogloBind™ Nucleic Acid/H0250-05
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产品说明
Suspension Reagent to remove Hemoglobin interference for PCRSuitable for infectious disease research or rigorous analysis for whole blood or heavily hemolyzed samplesScaleable suspension reagentRobust and simple, no phenol/chloroform or ammonium sulfate precipitationHigh Yield – free and soluble DNA or RNA is highly recoverable> 95% Hemoglobin and Protein removal from whole blood lysates & dried blood cardsEliminates inhibitory effects of heme for PCR applicationsCompatible with silica-type final isolation prepsSome infectious viruses are known or suspected to be associated with red cells, such as Zika and West Nile viruses. So to detect their presence, it is becoming increasingly apparent to use whole blood, rather than plasma alone, as a starting sample type. However, the presence of hemoglobin from whole blood lysates is challenging for PCR because of the large amount of hemoglobin present. HemogloBind™ Nucleic Acid is designed to efficiently remove hemoglobin while maintaining very high recovery of nucleic acids, both RNA and DNA.MATERIALSMaterials required5 ml 50 mlHemogloBind™ Nucleic Acid suppliedas Suspension Reagent in 10 % Isopropanol 5ml (25 prep)50ml (200 prep)Microfuge Spin-X type Filters25suppliedRecommended(Notsupplied)3M Guanidine ThioCyanate (orequivalent)NotsuppliedNotsuppliedWide bore pipette tipsSuggested(Not Supplied)Item No.HemogloBind™ Nucleic Acid 5ml (includes Spin filters)H0250-05HemogloBind™ Nucleic Acid 50ml (reagent only)H0250-50Click Here To View HemogloBind™ Product Sheet

    美国BSG(Biotech Support Group''LLC)公司是一家公司是一家全球知名的蛋白质组和基因组样本预处理的公司,是这个领域公认的领导者,该公司将其发明的专利表面化学技术应用生物学领域,比其他方法如亲合技术,苯酚法有绝对的优势。由于技术上的领先,使得BSG公司的产品广泛被美国国家基因组研究所,斯坦福大学等重要机构使用。(http://www.biotechsupportgroup.com/)

    Nature Article Cites HemogloBind™ and NRicher™ for Macromolecular Complex Annotation

    MONMOUTH JUNCTION, NJ, February 9, 2016 -- Biotech Support Group reports on a recent research article published in Nature, describing the simplicity and efficiency of their proteomic enrichment technology to observe and annotate soluble multiprotein complexes common to metazoa.


    The citation is:

    C Wan, B BorgesonS Phanse, F Tu, K DrewG Clark, et al. Panorama of ancient metazoan macromolecular complexesNature 525339–344 (17 September 2015).

    doi:10.1038/nature14877


    In brief, the article’s authors aimed at 
    elucidating the components of multiprotein complexes on a proteome-wide scale. The authors identify protein complexes from nine species in parallel, based on biochemical fractionation of native soluble macromolecular complexes followed by tandem mass spectrometry to identify components. The article states “…clarified heart and liver homogenates were frozen, and subsequently pooled, depleted of hemoglobin using HemogloBind™ according to manufacturer’s instructions…prior to sucrose gradient fractionation. A second Biotech Support Group product, formerly called SeraFILE PROspector and now trademarked as NRicher™ 6, was used for sub-proteome enrichment. The article states “Affinity bead based sample pre-separations were performed forC. elegans lysates as per manufacturer’s instructions…fractions (SeraFILE bead eluates) were subjected to ion exchange fractionation…”. The authors conclude that many complexes are conserved across species, and by correlating the results with genome sequence information, are able to predict more than one million interactions in 122 eukaryotes.

    I am very pleased that two of our products were able to contribute to this rigorous examination of protein complexes. When our products were used as a pretreatment step in the overall workflow, about twice the number of observations and annotations became possible. This further validates that the sub-proteome bias characteristics of NRicher™ 6 can simplify complex proteomes into less complex sub-proteomes with efficiencies suitable for deep functional proteome characterization. Furthermore, this study demonstrated the importance of a key feature implicit to all of our products; that is the maintenance of functional and structural integrity after separations. Without that particular feature, these additional observations would not have been possible.” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.