提醒:代购产品,无质量问题不接受退换货,下单前请仔细核对信息。下单后请及时联系客服 核对商品价格,订单生效后再付款。
Altogen/HepG2 Transfection Kit (Hepatocellular Carcinoma)/1.5 ml CRISPR/2154
价格:

自营商城

解放采购

正品保障

及时交付

厂家直采

一站服务

货号:
品牌:
会员服务:
尊享会员价
贵宾专线
运费优惠
闪电退款
福利优惠
上门换新
友情提示
以上价格仅为参考,请联系客服询价。
免费咨询热线
4000-520-616
产品说明
DescriptionProduct Availability: In Stock (delivery within 2-3 business days, no extra charge for drop-shipping).Ordering: To place an order via credit card or PO use Add to Cart button (creating an account is not required).Transfection Reagent for HepG2 Cells (Hepatocellular Carcinoma Cells, HB-8065)Proprietary cationic lipids formulationHigh transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNAEffective and robust intracellular deliveryKit includes Transfection Enhancer reagentProduces consistent results, lot-to-lot, plate-to-plate, and well-to-wellWork in the presence of serumA proven reagent for establishing stable cell linesOptimized transfection protocols are adapted for use with both standard & reverse transfection methodsDownload in vitro HepG2 transfection protocol: [PDF]Download HepG2 CRISPR/Cas9 transfection protocol: [PDF]Download PowerPoint presentation for HepG2 cells transfection kit: [PPT]Developed and manufactured by Altogen BiosystemsTransfection Efficiency:Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.Transfection Protocol and MSDS:Download Altogen Biosystems HepG2 Transfection Protocol: [PDF]Download MSDS: [PDF]HepG2 Cell Line:Liver cancer is highly aggressive and devastating disease that features several main characteristics, including a high rate of recurrence, low early detection rate and ineffective treatment approaches. In addition to being the fifth common malignancy, it is considered the second primary cause of cancer-related mortalities worldwide, as stated by Oncotarget, published in July 2015. The HepG2 hepatoma cell line is important for the toxicological assessment of novel combinatorial drugs. HepG2 was derived from the liver tissue of a 15-year-old male with differentiated hepatocellular carcinoma. These cells are epithelial and have a model chromosome number of 55. HepG2 cells secrete a variety of major plasma proteins, including albumin, transferrin, alpha 1-antitrypsin, alpha 2-macroglobulin, and plasminogen (they have been grown successfully in large-scale cultivation systems). The cells are responsive to stimulation with human growth hormone. HepG2 cells have also been shown to be resistant to G418. HepG2 cells and their derivatives are used as a model system for studies of liver metabolism and toxicity of xenobiotics, antigenotoxic and cogenotoxic agents, understanding hepatocarcinogenesis, the detection of cytoprotective, and for drug targeting studies. Also, HepG2 cells are commonly employed in trials with bio-artificial liver devices. This cell line is widely used in human cancer research of the liver and is essential for many different types of biomedical research. Altogen Biosystems offers highly efficient transfection reagent kits for the HepG2 hepatoma cell line.Data:Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the HepG2 cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).Figure 2. Protein expression of GAPDH in HepG2 cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into HepG2 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.Selected HepG2 transfection reagent product citations:Journal of Molecular Endocrinology 2010. Identification of protein kinases that control ovarian hormone release … Sirotkin et al [PDF]FEBS Letters 2009 (583). MicroRNA 125a and its regulation of the p53 tumor suppressor gene … Zhang et al [PDF]Cellular Physiology 2011. Identification of MicroRNAs Controlling Human Ovarian Cell Proliferation … Sirotkin et al [PDF]Selected in vivo transfection product citations (ALTOGEN® IN VIVO Transfection Kits used in the following publications):2008 454(7203):523-7. Innate immunity induced by composition-dependent RIG-I …Saito et al [PDF]Am J Pathology. 2010 177(4):1870-80. Role of ocular complement factor H in a murine model … Lyzogubov et al [PDF]Nature Biotechnology. 2011 29(4):341-5. Delivery of siRNA to the mouse brain by … Alvarez-Erviti et al [PDF]Cancer Research. 2011 71(15):5144-53. Inhibition of miR-193a expression by… Iliopoulos et al [PDF]2010 16(11):2108-19. RNase L releases a small RNA from HCV RNA that refolds … Malathi et al [PDF]2012 55(7):2069-79. The p47phox- and NADPH oxidase organiser 1 … Youn et al [PDF]British Journal of Cancer. 2012 107(3):516-26. TIGAR induces p53-mediated cell-cycle … Madan et al [PDF]2014 63(2):353-61. Tissue transglutaminase contributes to … Liu et al [PDF]Circulation Research. 2010 15;107(8). Kruppel-like factor-4 transcriptionally regulates … Cowan et al [PDF]2012 59(1):158-66. Role of uncoupled endothelial nitric oxide synthase … Gao et al [PDF]Jounal of Biological Chemistry. 2012 287(4):2907. Chaperoning of mutant p53 protein … Gogna et al [PDF]PLoS Pathogens. 2012 8(8) Uridine composition of the poly-U/UC tract of HCV RNA … Schnell et al [PDF]J Proteome Res. 2012(11) Retinal proteome analysis in a mouse model of oxygen-induced … Kim et al [PDF]2011 141(2) Differential type I interferon-mediated autophagic trafficking … Desai et al [PDF]PLoS Pathog. 2014 10(10) Exosomes from hepatitis C infected patients transmit HCV … Bukong et al [PDF] Request a Quote forHepG2 Transfection KitAltogen Biosystems: Altogen Biosystems is a life sciences company that manufactures over 100 cell type specific and pre-optimized transfection kits, electroporation buffers, and targeted in vivo delivery kits and reagents. Advanced formulation of reagents and optimized transfection protocols provide highly efficient intracellular delivery of biomolecules (proteins, DNA, mRNA, shRNA and siRNA, and small molecule compounds). Read more about transfection technology at Altogen’s Transfection Resource.Altogen Labs Research Services:Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com). Volume Options:0.5 ml (Catalog #2067)1.5 ml (Catalog #2068)1.5 ml CRISPR (Catalog #2154)8.0 ml (Catalog #2069)

Altogen Biosystems全国代理部分产品列表:

货号

产品

规格

价格(美元)

5030

 

 

 

1703

A204 Transfection Reagent (Rhabdomyosarcoma)

0.5ml

170

1704

A204 Transfection Reagent (Rhabdomyosarcoma)

1.5ml

348

窗体顶端

窗体底端

 

2101

A204 Transfection Reagent (Rhabdomyosarcoma)

1.5ml CRISPR

548

窗体顶端

窗体底端

 

7018

A204 Transfection Reagent (Rhabdomyosarcoma)

8.0ml

1044

窗体顶端

窗体底端

 

1167

A375 Cells Transfection Reagent (CRL-1619)

0.5ml

195

1168

A375 Cells Transfection Reagent (CRL-1620)

1.5ml

427

2102

A375 Cells Transfection Reagent (CRL-1621)

1.5ml CRISPR

627

1169

A375 Cells Transfection Reagent (CRL-1622)

8.0ml

1281

5030

Nanoparticle In Vivo Transfection Reagent

0.5 ml - 10 injections 

445

5010

LIPID In Vivo Transfection Reagent

0.5 ml - 10 injections

405

5040

PEG-Liposome In Vivo Transfection Reagent

0.5 ml - 10 injections

465

5020

POLYMER In Vivo Transfection Reagent

0.5 ml - 10 injections

425

5050

Pancreas In Vivo Transfection Kit

0.5 ml - 10 injections 

495

5060

Liver In Vivo Transfection Kit

0.5 ml - 10 injections 

495

5070

Kidney In Vivo Transfection Kit

0.5 ml - 10 injections 

495

1721

BMS2 Transfection Reagent (Bone Marrow Cells)

0.5 ml 

231

6345

C6 Transfection Reagent (Glioma Cells, CCL-107)

0.5 ml 

199

6803

DLD-1 Transfection Reagent (Colon Cancer, CCL-221)

0.5 ml 

201

1775

HUH-7 Transfection Reagent (Liver Cancer Cells)

0.5 ml 

179

客服在线
service-logo
已有 人查看该问题
tel
全国免费服务热线
4000-520-616
微信公众号
关注我们
手机扫码,关注动态