DescriptionProduct Availability: In Stock (delivery within 2-3 business days, no extra charge for drop-shipping).Ordering: To place an order via credit card or PO use Add to Cart button (creating an account is not required).Transfection Reagent for ZR-75-1 Cells (Breast Carcinoma Cells, CRL-1500)Proprietary cationic lipids formulationHigh transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNAWork in the presence of serumA proven reagent for establishing stable cell linesOptimized transfection protocols are adapted for use with both standard & reverse transfection methodsDownload in vitro ZR 751 transfection protocol: [PDF]Download ZR751 CRISPR/Cas9 transfection protocol: [PDF]Download PowerPoint presentation for ZR-75-1 cells transfection kit: [PPT]Developed and manufactured by Altogen BiosystemsTransfection Efficiency:Reagent exhibits at least 93% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.Transfection Protocol and MSDS:Download Altogen Biosystems ZR 751 Transfection Protocol: [PDF]Download MSDS: [PDF]ZR-75-1 Cell Line:Breast cancer is considered the most prevalent malignancy in females and the fifth most common cause of cancer-related fatalities among women worldwide, representing a significant public health challenge. Metastatic breast cancer is the primary cause of death in patients diagnosed with breast cancer. Preclinical cell line models could help in discovering innovative treatment options for those affected. The ZR-75- cell line is established from a 63-year-old Caucasian female with ductal carcinoma of the breast. This cell line expresses an estrogen receptor and possesses a hypertriploid chromosome number (polyploidy). In addition to producing high levels of MUC-1 mucin mRNA, the ZR-75-1 cell line has a population doubling time of nearly 80 hours. ZR-75 is an epithelial cell line and is useful for studying the properties of tumors in breast cancer patients. Altogen Biosystems offers highly efficient pre-optimized transfection kits for the ZR-75-1 cell line.Data:Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the ZR75 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).Figure 2. Protein expression of Cyclophilin B in ZR-75-1 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into ZR-75-1 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.Request a Quote for ZR-75-1 Transfection KitAltogen Biosystems: Altogen Biosystems provides pre-optimized transfection kits and electroporation products for life science research. Transfection products are developed for individual cancer cell line and transfection protocols are optimized to enable high transfection efficiency of biomolecules. Altogen Biosystems developed in vivo delivery products for small animal research, mouse and rat targeted tissue delivery: liver targeted, pancreas targeted, kidney targeted, PEG-Liposome-, Nanoparticle-, Lipid-, and Polymer-based in vivo transfection kits. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of biomolecules. Read more about transfection technology at Altogen’s Transfection Resource.Altogen Labs Research Services:Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com). Volume Options:0.5 ml (Catalog #6930)1.5 ml (Catalog #6931)1.5 ml CRISPR (Catalog #2203)8.0 ml (Catalog #7094)
Altogen Biosystems全国代理部分产品列表：
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产品
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规格
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价格（美元）
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5030
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1703
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A204 Transfection Reagent (Rhabdomyosarcoma)
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0.5ml
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170
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1704
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A204 Transfection Reagent (Rhabdomyosarcoma)
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1.5ml
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348
窗体顶端
窗体底端
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2101
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A204 Transfection Reagent (Rhabdomyosarcoma)
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1.5ml CRISPR
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548
窗体顶端
窗体底端
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7018
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A204 Transfection Reagent (Rhabdomyosarcoma)
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8.0ml
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1044
窗体顶端
窗体底端
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1167
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A375 Cells Transfection Reagent (CRL-1619)
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0.5ml
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195
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1168
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A375 Cells Transfection Reagent (CRL-1620)
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1.5ml
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427
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2102
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A375 Cells Transfection Reagent (CRL-1621)
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1.5ml CRISPR
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627
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1169
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A375 Cells Transfection Reagent (CRL-1622)
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8.0ml
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1281
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5030
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Nanoparticle In Vivo Transfection Reagent
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0.5 ml - 10 injections
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445
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5010
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LIPID In Vivo Transfection Reagent
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0.5 ml - 10 injections
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405
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5040
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PEG-Liposome In Vivo Transfection Reagent
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0.5 ml - 10 injections
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465
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5020
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POLYMER In Vivo Transfection Reagent
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0.5 ml - 10 injections
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425
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5050
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Pancreas In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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5060
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Liver In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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5070
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Kidney In Vivo Transfection Kit
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0.5 ml - 10 injections
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495
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1721
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BMS2 Transfection Reagent (Bone Marrow Cells)
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0.5 ml
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231
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6345
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C6 Transfection Reagent (Glioma Cells, CCL-107)
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0.5 ml
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199
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6803
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DLD-1 Transfection Reagent (Colon Cancer, CCL-221)
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0.5 ml
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201
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1775
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HUH-7 Transfection Reagent (Liver Cancer Cells)
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0.5 ml
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179
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