OverviewProduct nameHuman Cardiac Troponin I ELISA KitSee all Cardiac Troponin I kitsDetection methodColorimetricPrecisionIntra-assaySamplenMeanSDCV%Human heart83%Inter-assaySamplenMeanSDCV%Human heart37.1%Sample typeCell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasmaAssay typeSandwich (quantitative)Sensitivity 4.4 pg/mlRange31.3 pg/ml -4000 pg/mlRecoverySample specific recoverySample typeAverage %RangeSerum9793%- 101%Tissue Extracts112104%- 118%Cell culture media10297%- 108%Hep Plasma10799%- 117%EDTA Plasma9390%- 97%Cit plasma9387%- 100%Assay time1h30mAssay durationOne step assaySpecies reactivityReacts with:HumanProduct overviewAs of November 7, 2018, Human Cardiac Troponin 1 ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.Human Cardiac Troponin I ELISA Kit (ab200016) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cardiac Troponin I protein in serum, tissue extracts, cell culture extracts, hep plasma, cit plasma, edta plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cardiac Troponin I with 4.4 pg/ml sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.NotesThe regulatory troponin complex regulates skeletal and cardiac muscle contraction. This complex, together with tropomyosin, is located on the actin filament and it is composed of three protein subunits: troponin T (the tropomyosin-binding subunit), troponin I (the inhibitory subunit, which inhibits the ATPase activity of acto-myosin), and troponin C (the Ca2+-binding subunit). Troponins T and I have unique cardiac isoforms, whereas cardiac and skeletal muscle share troponin C. Specifically, three human troponin I isoforms have been described: one is expressed in cardiac muscle (Cardiac Troponin I) and the other two are found in slow-twitch and fast-twitch skeletal muscle fibers (slow sTnI and fast sTnI, respectively). The sequence homology between Cardiac Troponin I and slow sTnI is approximately 40% and somewhat less for fast sTnI. Cardiac Troponin I is 209 amino acid long with a molecular weight of approximately 24 kDa. Mouse and rat Cardiac Troponin I proteins both show 93% amino acid identity to human Cardiac Troponin I. The presence of human Cardiac Troponin I in serum (together with chest pain and electrocardiographic changes) is now considered as one highly specific biochemical marker of myocardial injury, risk stratification of acute coronary syndrome and myocardial infarction. Mutations of Cardiac Troponin I are associated with hereditary cardiomyopathy. Specifically, defects in Cardiac Troponin I are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intra-familial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in Cardiac Troponin I also cause cardiomyopathy familial restrictive type 1 (RCM1). RCM1 is a heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. Furthermore, cardiomyopathy dilated type 2A (CMD2A) and cardiomyopathy dilated type 1FF (CMD1FF), disorders characterized by ventricular dilation and impaired systolic function resulting in congestive heart failure and arrhythmia, are caused by defects in Cardiac Troponin I.Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.PlatformMicroplate (12 x 8 well strips)PropertiesStorage instructionsStore at +4°C. Please refer to protocols.Components1 x 96 tests10X Human Cardiac Troponin I Capture Antibody1 x 600µl10X Human Cardiac Troponin I Detector Antibody1 x 600µl10X Wash Buffer PT (ab206977)1 x 20ml5X Cell Extraction Buffer PTR (ab193970)1 x 10mlAntibody DiluentCPI - HAMA Blocker (ab193969)1 x 6mlHuman Cardiac Troponin I Lyophilized Recombinant Protein2 vialsPlate Seals1 unitSample Diluent 50BP1 x 20mlSample Diluent NS (ab193972)1 x 50mlSimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unitStop Solution1 x 12mlTMBDevelopment Solution1 x 12mlCellular localizationCytosol; Sarcomere.Alternative namesCardiomyopathy dilated 2A (autosomal recessive)CMD1FFCMD2ACMH7cTnIRCM1TNNC1TNNI3Troponin I cardiac muscleTroponin I type 3 (cardiac)see allDatabase linksEntrez Gene:7137 HumanOmim:191044 HumanSwissProt:P19429 Human