产品说明
DescriptionProduct nameStreptavidin (HRP)See all Streptavidin proteins and peptidesBiological activityBinds to Biotin. Dissociation constant has not been measured.Expression systemNativeAccessionP22629Protein lengthFull length proteinAnimal freeNoNatureNativeSpeciesStreptomyces avidiniiConjugationHRPDescriptionNative Streptavidin protein (HRP)Associated productsSubstrate reagentABTS™, Peroxidase substrate (ab142041)SpecificationsOur Abpromise guarantee covers the use ofab7403in the following tested applications.The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.ApplicationsDot blotELISAImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)Immunohistochemistry (Frozen sections)ImmunomicroscopyImmunocytochemistryWestern blotFormLiquidAdditional notesHorseradishperoxidase is conjugated to the streptavidin tetramer at ~1:1 molar ratio.This product has been assayed against 1.0 µg of Biotinylated IgG in a standard capture ELISA using a peroxidase substrate as ABTS ab142041 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.Streptavidin-HRP was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.Concentration information loading...Preparation and StorageStability and StorageShipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.Preservative: 0.01% Gentamicin sulphateConstituents: 0.424% Potassium phosphate, 0.87% Sodium chloride, BSAThis product is an active protein and may elicit a biological response in vivo, handle with caution.General InfoAlternative namesSA V1SA V2strepavidinStreptavidin V1Streptavidin V2see allRelevanceStreptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.Cellular localizationCytoplasmic
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