OverviewProduct nameFirePlex® miRNA Panel – Cardiology V2Detection methodFlow cytometry-fluorescentAssay typeMultiplexAssay time4h00mSpecies reactivityReacts with:Mouse, Rat, HumanProduct overviewMultiplex miRNA Assay - Cardiology Panel (ab218368) are the FirePlex® particles designed to profile up to 65 literature curated miRNA for cardiology studies, miRNAsdifferentially regulated in normal or abnormal cardiovascular health.NOTE: FirePlex miRNA panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835)to select the most appropriate Run Buffer for your flow cytometer.Our multiplex miRNA assays use our FirePlex® particle technologywith the same sensitivity as PCR, they can be used either directlywith 10 µL of biofluids such as serum or plasma with no need for RNA purification; or with 100 pg of purified RNA.Find a full list of validated sample types, and learn more about the performance and requirements of these assays,here.These miRNAs have been chosen based on a detailed study of data in peer-reviewed journals and exhibit differential regulation in whole blood, plasma, serum, peripheral blood mononuclear cells (PBMCs) or isolated exosomes.Included in the panel are general markers for multiple cardiovascular-related diseases, markers specific to specific cardiovascular states or treatment responses, markers that may indicate hemolysis contamination in isolated plasma/serum/exosomes and internal controls to validate assay performance.It is important to use the normalization procedure in the FirePlex® Analysis Workbench Softwareto identify the miRNAs that can optimally be used for normalization with your samples. Three miRNAs that are likely to be stably expressed in biofluid samples and may be suitable have been included in this panel.NotesTo use this product researchers must also purchase the appropriate Core Reagent Kit, either ab218365 for use with purified RNA or ab218342 for use withbiofluids.FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.** Hu = Human, Ms = Mouse, Rt = Rat, Ath = Arabidopsis thaliana, Oan = Ornithorhynchus anatinus, Cel = Caenorhabditis elegans.#miRNA 21 namemiRNA Sequence (miRBase V21)Sequence homology **MIMAT ID1hsa-let-7d-5pagagguaguagguugcauaguuHu, Ms, RtMIMAT00000652hsa-let-7e-5pugagguaggagguuguauaguuHu, Ms, RtMIMAT00000663hsa-let-7g-5pugagguaguaguuuguacaguuHu, Ms, RtMIMAT00004144hsa-let-7i-5pugagguaguaguuugugcuguuHu, Ms, RtMIMAT00004155hsa-miR-1-3puggaauguaaagaaguauguauHu, Ms, RtMIMAT00004166hsa-miR-103a-3pagcagcauuguacagggcuaugaHu, Ms, RtMIMAT00001017hsa-miR-106b-5puaaagugcugacagugcagauHu, Ms, RtMIMAT00006808hsa-miR-122-5puggagugugacaaugguguuugHu, Ms, RtMIMAT00004219hsa-miR-124-3puaaggcacgcggugaaugccHu, Ms, RtMIMAT000042210hsa-miR-125a-5pucccugagacccuuuaaccugugaHu, Ms, RtMIMAT000044311hsa-miR-125b-5pucccugagacccuaacuugugaHu, Ms, RtMIMAT000042312hsa-miR-126-3pucguaccgugaguaauaaugcgHu, Ms, RtMIMAT000044513hsa-miR-133a-3puuugguccccuucaaccagcugHu, Ms, RtMIMAT000042714hsa-miR-133buuugguccccuucaaccagcuaHu, Ms, RtMIMAT000077015hsa-miR-142-3puguaguguuuccuacuuuauggaHu, Ms, RtMIMAT000043416hsa-miR-144-5pggauaucaucauauacuguaagHuMIMAT000460017hsa-miR-145-5pguccaguuuucccaggaaucccuHu, Ms, RtMIMAT000043718hsa-miR-146a-5pugagaacugaauuccauggguuHu, Ms, RtMIMAT000044919hsa-miR-150-5pucucccaacccuuguaccagugHu, Ms, RtMIMAT000045120hsa-miR-155-5puuaaugcuaaucgugauagggguHuMIMAT000064621hsa-miR-15b-5puagcagcacaucaugguuuacaHu, Ms, RtMIMAT000041722hsa-miR-16-5puagcagcacguaaauauuggcgHu, Ms, RtMIMAT000006923hsa-miR-17-5pcaaagugcuuacagugcagguagHu, Ms, RtMIMAT000007024hsa-miR-181b-5paacauucauugcugucgguggguHu, Ms, RtMIMAT000025725hsa-miR-18a-5puaaggugcaucuagugcagauagHu, Ms, RtMIMAT000007226hsa-miR-192-5pcugaccuaugaauugacagccHu, Ms, RtMIMAT000022227hsa-miR-194-5puguaacagcaacuccauguggaHu, Ms, RtMIMAT000046028hsa-miR-195-5puagcagcacagaaauauuggcHu, Ms, RtMIMAT000046129hsa-miR-199a-3pacaguagucugcacauugguuaHu, Ms, RtMIMAT000023230hsa-miR-199a-5pcccaguguucagacuaccuguucHu, Ms, RtMIMAT000023131hsa-miR-19a-3pugugcaaaucuaugcaaaacugaHu, Ms, RtMIMAT000007332hsa-miR-208a-3pauaagacgagcaaaaagcuuguHu, MsMIMAT000024133hsa-miR-208b-3pauaagacgaacaaaagguuuguHu, MsMIMAT000496034hsa-miR-20b-5pcaaagugcucauagugcagguagHu, Ms, RtMIMAT000141335hsa-miR-210-3pcugugcgugugacagcggcugaHu, Ms, RtMIMAT000026736hsa-miR-214-3pacagcaggcacagacaggcaguHu, MsMIMAT000027137hsa-miR-21-5puagcuuaucagacugauguugaHu, Ms, RtMIMAT000007638hsa-miR-25-3pcauugcacuugucucggucugaHu, Ms, RtMIMAT000008139hsa-miR-26a-5puucaaguaauccaggauaggcuHu, Ms, RtMIMAT000008240hsa-miR-26b-5puucaaguaauucaggauagguHu, Ms, RtMIMAT000008341hsa-miR-27a-3puucacaguggcuaaguuccgcHu, Ms, RtMIMAT000008442hsa-miR-27b-3puucacaguggcuaaguucugcHu, Ms, RtMIMAT000041943hsa-miR-28-5paaggagcucacagucuauugagHu, Ms, RtMIMAT000008544hsa-miR-29a-3puagcaccaucugaaaucgguuaHu, Ms, RtMIMAT000008645hsa-miR-29b-3puagcaccauuugaaaucaguguuHu, Ms, RtMIMAT000010046hsa-miR-30a-5puguaaacauccucgacuggaagHu, Ms, RtMIMAT000008747ath-mir167dugaagcugccagcaugaucuggAthMIMAT000090548hsa-miR-320baaaagcuggguugagagggcaaHuMIMAT000579249hsa-miR-328-3pcuggcccucucugcccuuccguHu, Ms, RtMIMAT000075250hsa-miR-335-5pucaagagcaauaacgaaaaauguHu, Ms, RtMIMAT000076551hsa-miR-337-5pgaacggcuucauacaggaguuHuMIMAT000469552hsa-miR-342-3pucucacacagaaaucgcacccguHu, Ms, RtMIMAT000075353hsa-miR-34a-5puggcagugucuuagcugguuguHu, Ms, RtMIMAT000025554hsa-miR-363-3paauugcacgguauccaucuguaHuMIMAT000070755hsa-miR-370-3pgccugcugggguggaaccugguHu, Ms, RtMIMAT000072256oan-miR-7417-5puuccccacucugagcacacagcOanMIMAT002898257hsa-miR-375uuuguucguucggcucgcgugaHu, Ms, RtMIMAT000072858cel-mir-70-3puaauacgucguugguguuuccauCelMIMAT000004259hsa-miR-423-5pugaggggcagagagcgagacuuuHu, MsMIMAT000474860hsa-miR-433-3paucaugaugggcuccucgguguHu, Ms, RtMIMAT000162761hsa-miR-451aaaaccguuaccauuacugaguuHu, Ms, RtMIMAT000163162hsa-miR-485-3pgucauacacggcucuccucucuHuMIMAT000217663hsa-miR-486-5puccuguacugagcugccccgagHu, Ms, RtMIMAT000217764hsa-miR-499a-5puuaagacuugcagugauguuuHu, Ms, RtMIMAT000287065hsa-miR-505-5pgggagccaggaaguauugauguHuMIMAT000477666hsa-miR-590-5pgagcuuauucauaaaagugcagHu, MsMIMAT000325867hsa-miR-92a-3puauugcacuugucccggccuguHuMIMAT000009268hsa-miR-93-5pcaaagugcuguucgugcagguagHu, Ms, RtMIMAT000009369x-controlx-controlN/A70blankblankN/APlatformMultiplexPropertiesStorage instructionsStore at +4°C. Please refer to protocols.Components1 x 48 tests1 x 96 testsFirePlex®Particle Mix (Circulating)1 x 1750µl1 x 3500µl