OverviewProduct nameTGF Beta FirePlex®-HT PanelDetection methodFluorescentSample typeCell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasmaAssay typeMultiplexSpecies reactivityReacts with:Mouse, Rat, Human, Non human primatesPredicted to work with:DogProduct overviewTGF Beta FirePlex®-HT Panel (ab270739) uses FirePlex® particle technology to detect TGF-B1, TGF-B2, and TGF-B3 from 6.25 µl of sample input in 384-well plate format.Assays are validated in serum, plasma, and cell culture supernatant, and can be run with 175 samples in duplicate per 384-well plate.The two-step workflow and no-wash assay format limit hands-on time and are amenable to automation, thus making FirePlex-HT ideally suited for high-throughput screening studies. Assay readout is conducted on high-content imagers with data analysis using the FirePlex® Analysis Workbench software.Users should note that assay performance data supplied in the accompanying Product Datasheet is a representative dataset, and is not lot-specific. For lot-specific assay performance data, please refer to the product"s certificate of analysis, which can be foundhere.FirePlex® is a registered trade mark in certain territories (including Europe and the United States) and is an unregistered trade mark elsewhere.NotesThis product is currently only supported for customers in North America and Europe, if you are outside these areas pleasecontact us.The followingtable provides the sensitivity, dynamic range, and CVs for TGF Beta FirePlex®-HT Panel 1*.TargetSensitivity/LOD(pg/ml)Dynamic Range(pg/ml)Inter-well CV(Std curve)TGF-B16.3913.72- 3,3336.1%TGF-B248.63123.46- 30,0005.0%TGF-B327.5741.15- 10,0005.7%*representative dataset, not lot-specific. For lot-specific assay performance data, please refer to the product"s certificate of analysis, which can be foundhere.PlatformMultiplexPropertiesStorage instructionsPlease refer to protocols.Components1 x 384 tests384 Well Imaging Plate1 unit384 Well Imaging Plate Lid1 unit384 Well Imaging Plate Seal2 unitsFirePlex-HT 1X PBS1 x 8mlFirePlex-HT 5X Capture Particle Mix1 x 700µlFirePlex-HT Protein Standard Mix HTTG1 x 7 testsFirePlex-HT Reagent Diluent R21 x 8mlFirePlex-HT Sample Diluent S11 x 25mlFirePlex-HT- Imaging Dye Concentrate1 x 875µlFirePlex-HT35X Detector Antibody Mix1 x 110µlResearch areasCardiovascularAngiogenesisGrowth FactorsTGFSignal TransductionGrowth Factors/HormonesTGFStem CellsSignaling PathwaysTGF betaSecretedCancerGrowth factorsTGFCancerInvasion/microenvironmentAngiogenesisAngiogenic growth factorsCancerCancer MetabolismResponse to hypoxiaMetabolismPathways and ProcessesCofactors, Vitamins / mineralsCo-factorsMetabolismPathways and ProcessesMetabolism processesHypoxiaCellular localizationTGF beta 1: Secreted > extracellular space > extracellular matrix.TGF beta 2: Secreted.TGF beta 3: Secreted.Database linksEntrez Gene:7040 HumanEntrez Gene:7042 HumanEntrez Gene:7043 HumanEntrez Gene:21803 MouseEntrez Gene:21808 MouseEntrez Gene:21809 MouseEntrez Gene:25717 RatEntrez Gene:59086 RatEntrez Gene:81809 RatOmim:190180 HumanOmim:190220 HumanOmim:190230 HumanSwissProt:P01137 HumanSwissProt:P10600 HumanSwissProt:P61812 HumanSwissProt:P04202 MouseSwissProt:P17125 MouseSwissProt:P27090 MouseSwissProt:P17246 RatSwissProt:Q07257 RatSwissProt:Q07258 RatUnigene:133379 HumanUnigene:592317 HumanUnigene:645227 HumanUnigene:713281 HumanUnigene:18213 MouseUnigene:248380 MouseUnigene:3992 MouseUnigene:24539 RatUnigene:40136 RatUnigene:7018 Ratsee all