DescriptionProduct nameRecombinant human IGF1 protein (Active)See all IGF1 proteins and peptidesBiological activityFully biologicaly active as determined by dose-depended proliferation of MCF-7 cells.ED50 is ≤ 0.9 ng/mL, corresponding to a specific activity of 1.11 x 106 units/mg.Purity>= 95% Immunogen affinity purified.Purity by HPLC >=95%.Endotoxin levelExpression systemHEK 293 cellsAccessionP05019Protein lengthFull length proteinAnimal freeYesCarrier freeYesNatureRecombinantSpeciesHumanSequenceGP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSAPredicted molecular weight8 kDaMolecular weight informationM - 2.84 Da (Calculated mass 7711.84 Da)GP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSAAmino acids49 to 118Additional sequence informationN-terminal glycineFull-length mature chain lacking the signal peptide and both pro-peptides.Associated productsSpecificationsOur Abpromise guarantee covers the use ofab270062in the following tested applications.The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.ApplicationsCell CultureSDS-PAGEHPLCMass SpectrometryFunctional StudiesFormLyophilizedAdditional notesThis protein is filter sterilized prior to aliquoting and lyophilization. All aliquoting and lyophilization steps are performed in a sterile environmentConcentration information loading...Preparation and StorageStability and StorageShipped at Room Temperature. Store at Room Temperature.Information available upon request.This product is an active protein and may elicit a biological response in vivo, handle with caution.ReconstitutionReconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.General InfoAlternative namesIBP1IGF IIGF IAIGF IBIGF-IIgf1IGF1_HUMANIGF1AIGFIIGFIAInsulin like growth factor 1Insulin like growth factor 1 (somatomedin C)Insulin like growth factor IAInsulin like growth factor IBInsulin-like growth factor IMechano growth factorMGFOTTHUMP00000195080OTTHUMP00000195081OTTHUMP00000195082OTTHUMP00000195083OTTHUMP00000195084Somatomedia CSomatomedin CSomatomedin-Csee allFunctionThe insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.Involvement in diseaseDefects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.Sequence similaritiesBelongs to the insulin family.Cellular localizationSecreted.Target information above from: UniProt accessionP05019The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).Information by UniProt