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echelon-inc/CDP-ME Synthase (IspD) Enzymatic Assay/1ea/K-2000D-1ea
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 CatalogNo.: K-2000DDescription: TheCDP-MESynthase(IspD)EnzymaticAssayincludesallofthenecessarycomponentstoeffectivelymonitortheactivityoftheIspDenzyme.PyrophosphateisabyproductfromtheformationofCDP-MEwhichisthenconvertedtofreephosphateintheassaybypyrophosphatase.BymeasuringfreephosphatelevelstheusercaninfertheamountofMEPconvertedtoCDP-ME.Isoprenoidscompriseoneofthemostdiverseclassesofcompoundsfoundinnature.Withover50,000differentisoprenoidsidentifiedtodate,theyexhibitabroadrangeofstructuralcomplexityandareinvolvedinavarietyofBIOLOGicalfunctions.Electrontransport(quinones),stABIlizationofcellmembranes(hopanoidsandsterols),cellwallbiosynthesis(dolichols),signaltransduction(prenylatedproteins),photosynthesis(chlorophylls)andmodificationoftRNAsareamongtheprocessesthatinvolveisoprenoids.Isopentenyldiphosphate(IPP)anddimethylallyldiphosphate(DMAPP)aretheprecursorsforallisoprenoidcompoundsandtwounrelatedessentialpathwaysexistinnaturefortheirbiosynthesis.ThesetwoprecursorsareproducedbyeithertheMVAorMEPpathway.TheMVApathwayisfoundprimarilyineukaryotesandarchea,includinghumans,plantcytosolandsomeGram-positivebacteria,whiletheMEPpathwayisutilizedbymostbacteria,includingallGram-negatives,andplantchloroplasts.Duetothisnaturaldistribution,theMEPpathwayrepresentsapromisingtargetfordevelopmentofnovelantibacterialagentsandherbicides.InthesecondcommittedstepoftheMEPpathway,MEPisconvertedto2-C-methyl-D-erythritrol4-phosphate(CDP-ME)byCDP-MEsynthase(IspD,alsoreferredtoasYgbP)withtheconcomitantformationofinorganicpyrophosphate(PPi).TheassayprovidedinthiskitincludesinorganicpyrophosphatasewhichconvertsthePPigeneratedduringIspDcatalysistoinorganicphosphate(Pi).PiisdetectedspectrophotometricallyusingamalachitegreensolutionStorageKitcanbestoredunopenedat-20oCforuptosixmonths.Allcomponentsandsolutionsshouldbeprotectedfromlight.RelatedProductsMEPPathwayReagentsIsoprenoidsMEPPathwayinhibitors:Fosmidomycin(B-4201),FR900098(K-4202),5-Ketoclomazone(K-4101)CDP-MEPSynthesiskit(K-2000E)MEPPathwayEnzymes**COMINGSOON**:DXPSynthase,MEPSynthase,IspDEnzyme,IspEEnzyme,IspFEnzyme

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