DescriptionViPrimePLUS Lyophilized One Step qRT-PCR Master Mix is next generation first choice mix for a convenient and rapid one step real-time PCR reaction set up. The master mix has been freeze-dried to produce a room temperature stable preparation. The freeze-dried master mix contains unique thermostable M-MULV enzyme, Taq DNA polymerases, MgCl2 and buffer components at optimal concentration. ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix has same optimal performance as ViPrimePLUS One Step qRT-PCR Master Mix and ViPrimePLUS One Step qRT-PCR Green Master Mix. The lyophilized qRT-PCR Master Mix can detect extremely low copy number targets of RNA templates in one step complete system. The removal of separate reverse transcription step not only reduces errors and time to obtain results, but also leads the qRT-PCR reaction to optimum sensitivity and specificity. ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix is used with supplied re-suspension buffer and ROX dye; hence hardware platforms that need ROX as passive reference dye can be used in standard cycling conditions.FeaturesConvenient and rapid real time PCR reaction set upLyophilized form – room temperature stable preparationEquipped with thermostable M-MULV enzyme and Taq DNA PolymeraseReliable quantification of low-abundance transcripts very specifically with high efficiencyCompatible with most of the real-time PCR platformsKit Component3 x Lyophilized qPCR MasterMix1 x Lyophilized ROX4 x Re-suspension buffer Master Mixes Selection Guidelines for System CompatibilityTo calibrate a real-time PCR reaction, the various formulations of Master Mixes are available for most of the platforms.Real-time PCR platformsROX re-suspension volumeROX addition per tube of master mixApplied Biosystems 7000, 7700, 7900, 7300 StepOne, StepOnePLUS and ViA7 platforms, Roche capillary Lightcyclers.100µl20µlAll Stratagene platforms200µl15µlApplied Biosystems 7500 platform700µl10µlAll other machinesNot RequiredNot RequiredOrdering InformationCatalog NoDescriptionPack SizeQLMM06ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix150 reactionsDownloadManualViPrimePLUS Lyophilized One Step qRT-PCR Master Mix