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Kerafast/Anti-Actin-7 Antibodies/EGA247/100ug
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ThesemousemonoclonalantibodiesweregeneratedagainstArABIdopsisthalianaActin-7andrecognizeA.thalianaActin-7.Highlights:ReactwithA.thalianaActin-7(Maydisplayreactivitytowardmultipleotherespecies)SuitableforWesternBlot,ELISAandImmunofluorescenceapplicationsActin-7hasbeenidentifiedinanimals,plantsandprotists.Duringplantgrowthanddevelopment,thephytohormoneauxininducesawidearrayofchangesthatincludecelldivision,cellexpansion,celldifferentiation,andorganinitiation.IthasbeensuggestedthattheactinCytoskeletonplaysanactiveroleintheelaborationoftheseresponsesbydirectingspecificchangesincellmorphologyandcytoarchitecture.ThepromoterandproteinproductofACT7,anAraBDopsisvegetativeactingene,arerapidlyandstronglyinducedinresponsetoexogenousauxin.ACT7isrequiredfortheformationofcallustissue,andisalsoessentialforgerminationandrootgrowthACT7actingeneisalsoexpressedinrapidlydevelopingtissuesandrespondstoexternalstimuli,suchasexposuretohormones.FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.SpecificationsAnti-Actin-7AntibodiesProductType: AntibodyName: Actin-7AntibodiesAccessionID: P53492Antigen: Actin-7Isotype: IgGClonality: MonoclonalCloneName: 29G12.G5.G6,33E8.C11.F5.D1,36H8.C12.H10.B6Reactivity: TestedinA.thaliana.Maydisplayreactivitytowardmultipleotherespecies(ND)Immunogen: A.thalianaActin-7SpeciesImmunized: BALB/cmousePurificationMethod: ProteinGBuffer: 0.1MSodiumPhosphate,pH7.4,0.15MNaCl,0.05%(w/v)SodiumAzideTestedApplications: WB,ELISA,IF(ItisadvisabletouseallthreeMAbsinthefirst-time,qualitativeexperimentalsetuptodeterminewhichisthemostsuitableforaspecificexperiment.ThesemaybeusedyocomplementexcperimentscarriedoutinvolvingmAbGEauniversalanti-actinsantibody.)Storage: -20CShipped: ColdpacksProviderFromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.CommentsItisadvisabletouseallthreeMAbsinthefirst-time,qualitativeexperimentalsetuptodeterminewhichisthemostsuitableforaspecificexperiment.ThesemaybeusedyocomplementexcperimentscarriedoutinvolvingmAbGEauniversalanti-actinsantibody.ReferencesKandasamy,M.K.,Gilliland,L.U.,McKinney,E.C.,andMeagher,R.B.(2001).Oneplantactinisovariant,ACT7,isinducedbyauxinandrequiredfornormalcallusformation.PlantCell13,1541-54.Kandasamy,M.K.,McKinney,E.C.,andMeagher,R.B.(1999).Thelatepollen-specificactinsinangiosperms.PlantJ18,681-91.Kandasamy,M.K.,McKinney,E.C.,Roy,E.,andMeagher,R.B.(2012).Plantvegetativeandanimalcytoplasmicactinssharefunctionalcompetenceforspatialdevelopmentwithprotists.PlantCell24,2041-57.L.U.Gilliland,L.C.Pawloski,M.K.Kandasamy,andR.B.Meagher,“ArabidopsisactingeneACT7playsanessentialroleingerminationandrootgrowth,”PlantJournal,vol.33,no.2,pp.319–328,2003.McDowell,JM,An,Y-q,Huang,S,McKinney,EC,MeagherRB:TheArabidopsisACT7ActinGeneIsExpressedinRapidlyDevelopingTissuesandRespondstoSeveralExternalStimuli.PlantPhysiol.(1996)111:699-711.Ifyoupublishresearchwiththisproduct,pleaseletusknowsowecanciteyourpaper.

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