Clipboard, Search History, and several other advanced features are temporarily unavailable. Serotyping of foot-and-mouth disease virus by antigen capture-ELISA using monoclonal antibodies and chicken IgY 1 Research and Development Centre, Indian Immunologicals Limited, Gachibowli, Hyderabad, India. Serotyping of foot-and-mouth disease virus by antigen capture-ELISA using monoclonal antibodies and chicken IgY 1 Research and Development Centre, Indian Immunologicals Limited, Gachibowli, Hyderabad, India. Laboratory detection of specific foot-and-mouth disease virus (FMDV) is routinely carried out by ELISA and RT-PCR. Identification and serotyping of FMDV by ELISA requires polyclonal antibodies raised in rabbits and guinea pigs. The polyclonal antibodies have certain disadvantages such as batch to batch variation, inconsistent yields of antibodies and limited quantity of serum obtained from individual animals. This paper describes a method wherein monoclonal antibodies and chicken IgY were used in an antigen capture-ELISA for serotyping of thirty tongue epithelial samples and sixty tissue culture fluids. The results were compared with the routine antigen detection ELISA. The present study indicated that monoclonal antibodies and chicken IgY can substitute conventional polyclonal antibodies for routine serotyping of FMDV. Muller JD, McEachern JA, Bossart KN, Hansson E, Yu M, Clavijo A, Hammond JM, Wang LF. Muller JD, et al. J Virol Methods. 2008 Jul;151(1):146-53. doi: 10.1016/j.jviromet.2008.03.011. Epub 2008 Apr 25. J Virol Methods. 2008. PMID: 18440078 Morioka K, Fukai K, Yoshida K, Yamazoe R, Onozato H, Ohashi S, Tsuda T, Sakamoto K. Morioka K, et al. J Clin Microbiol. 2009 Nov;47(11):3663-8. doi: 10.1128/JCM.00695-09. Epub 2009 Sep 16. J Clin Microbiol. 2009. PMID: 19759230 Free PMC article. Ko YJ, Jeoung HY, Lee HS, Chang BS, Hong SM, Heo EJ, Lee KN, Joo HD, Kim SM, Park JH, Kweon CH. Ko YJ, et al. J Virol Methods. 2009 Jul;159(1):112-8. doi: 10.1016/j.jviromet.2009.03.011. Epub 2009 Mar 20. J Virol Methods. 2009. PMID: 19442854 Pearson GR. J Virol Methods. 1988 Sep;21(1-4):97-104. doi: 10.1016/0166-0934(88)90056-0. J Virol Methods. 1988. PMID: 2846624 Wong CL, et al. Front Vet Sci. 2020 Aug 21;7:477. doi: 10.3389/fvets.2020.00477. eCollection 2020. Front Vet Sci. 2020. PMID: 32974392 Free PMC article. Review.