Details
Stu I Source
Streptomyces tubercidicus
Reagents Supplied
CMX Buffer 2
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of umethylated lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl
Star Activity
No
Reaction Temperature
37°C
Heat Inactivation
10 minutes at 65°C
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 2:10 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl250 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1:75%Buffer 2:100%Buffer 3: 50%Buffer 4: 75%Buffer 5: 75%
Storage Buffer
10 mM Tris‑HCl (pH 7.4 at 4°C)50 mM KCl0.1 mM EDTA1 mM dithiothreitol0.5 mg/ml bovine serum albumin0.1 mM PMSF50% (v/v) glycerol
Assay Conditions
10 mM Tris-HCl (pH 7.5 at 37°C)50 mM NaCl10 mM MgCl21 mM dithiothreitol100 µg/ml bovine serum albumin1 µg unmethylated lambda DNAIncubation is at 37°C for 1 hour in a total reaction volume of 50 µl
Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of AnalysisPDF - Current LotMSDS PDF- Current Lot
Categories
Restriction Endonucleases
