DescriptionHCII – Heparin Cofactor II Deficient PlasmaHCII – Heparin Cofactor II Deficient Plasma is manufactured from normal citrated human plasma that has been depleted of HCII – Heparin Cofactor II Deficient Plasma by selective affinity immuno-adsorption using antibodies directed towards HCII.  Available in two different formats – Frozen and LyophilizedOnly the highest quality citrated plasma is used as starting material and in many cases the parent plasma is available as control material.  Our HCII – Heparin Cofactor II Deficient Plasma can be used for further manufacturing or research use only applications.Frozen HCII Deficient PlasmaProduct Code:  HCII-DPPresentation:  Frozen HCII – Heparin Cofactor II Deficient PlasmaPreparation/Handling:  Thaw 1 ml vials in 37oC water bath for 5 minutes; for bulk volumes, thawing time will be dependent on bottle size.Storage and Stability:  Plasma is shipped frozen and should be stored below -60oC. Product is stable until date stated on vial label when stored at -60oC. Once thawed, plasma is stable for 4 hours at 2-8oC in original vial.Certificate of Analysis:  available upon requestLyophilized HCII Deficient PlasmaProduct Code:  HCII-LDPPresentation:  Freeze Dried HCII – Heparin Cofactor II Deficient Plasma containing 50 mM HEPES and stabilizers.Reconstitution: Reconstitute with 1 mL of reagent grade water.  Allow contents to dissolve for 15 minutes at room temperature with occasional swirling.  Stability after reconstitution is 4 hours at ambient (18-25oC), or 30 days at –20oC.Storage and Stability:  Prior to reconstitution, dried plasma should be stored at 2-8°C.  Product is stable until date stated on vial label when stored at 2-8oC.  Stability after reconstitution is 4 hours at ambient (18-25oC), or 30 days at –20oC.Certificate of Analysis:  available upon requestDescription of Heparin CoFactor IIHeparin Cofactor II (HCII), also known as heparin cofactor A and dermatan sulphate cofactor, is a single chain glycoprotein produced in the liver and circulates in plasma at concentration of 50-100 μg/ml (0.8-1.5 μM). A member of the SERPIN family of proteinase inhibitors, HCII exhibits a relatively narrow specificity and is only known to inhibit the serine proteinases thrombin and chymotrypsin. The inhibitory activity toward thrombin is accelerated up to 1000-fold in the presence of optimal concentrations of heparin. Unlike Antithrombin, thrombin inhibition by HCII is also enhanced by dermatan sulphate. HCII has an apparent molecular weight of 65,600 daltons and interaction with thrombin results in a covalent 1:1 enzyme-inhibitor complex of 102,600 daltons 1-3.References and Reviews Tollefsen DM, Majerus DW, Blank MK; Heparin Cofactor II; JBC 257:5, p2162-2169, 1982.Pratt CW, Whinna HC, Mead JB, Treanor RE, Church FC: Physicochemical aspects of Heparin Cofactor II; Ann N.Y. Acad. Sci.: 556, p104-115, 1989.Griffith MJ, Noyes CM, Church FC; Reactive Site Peptide Structural Similarity between Heparin Cofactor II and Antithrombin III; JBC:260, pp 2218-2225, 1985.Related Products:HCII – Heparin Cofactor II Polyclonal AntibodyGAHC2-IG – Goat anti human Heparin Cofactor II, purified IgG (5mg vial)Heparin Cofactor II Polyclonal Antibody – Affinity PurifiedGAHC2-AP – Goat anti human Heparin Cofactor II, affinity purified IgG (0.5 mg vial)Heparin Cofactor II Polyclonal Antibody – Affinity Purified – HRP ConjugatedGAHC2-APHRP – Goat anti human Heparin Cofactor II, affinity purified, peroxidase conj. IgG (0.1 mg vial)HCII – Heparin Cofactor II Deficient PlasmaHCII-DP – Frozen Heparin Cofactor II Deficient Plasma – 1ml or bulk quantitiesHCII-LDP – Lyophilized Heparin Cofactor II Deficient Plasma – 1ml