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PBL/Cynomolgus/Rhesus IFN Alpha ELISA Kit (Serum, Plasma, TCM)/1 x 96-well plate/46100-1
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Cynomolgus/Rhesus IFN Alpha ELISA Kit (Serum, Plasma, TCM)Home / Products / Assay Kits / ELISAs / Non-Human ELISAs / Cynomolgus/Rhesus IFN Alpha ELISA Kit (Serum, Plasma, TCM)Product Features:Quantifies Cynomolgus and Rhesus IFN-Alpha 2 in serum, plasma, and tissue culture media (TCM)Reproducible results with tissue culture media inter-assay CV ≤ 9.2% and intra-assay CV ≤ 2.5% (serum ≤ 14.4% and ≤ 9.2%, respectively)Recombinant Rhesus/Cyno IFN Alpha 2 provided as the ELISA standardFull Product Name: VeriKine Cynomolgus/Rhesus Interferon Alpha Serum ELISA Kit OrderingCatalog No. Pack Size Price Quantity 46100-1 1 x 96-well plate$785.00 SpecificationsTech Info / DataCitationsDocumentationSpecificationsCompatibility: Serum, Plasma, Cell Culture SupernatantAssay Range: 25 - 1600 pg/mlSpeed: Incubation time, 3 hours, 15 minutesSpecificity: Cynomolgus (Macaca fascicularis) and Rhesus (Macaca mulatta) Interferon Alpha 2Strong cross-reactivity with human IFN-α2. No cross-reactivity against human IFN-γ, mouse or rat IFN-α. Inter-Assay CV: < 9.2% (TCM); 14.4% (serum)Intra-Assay CV: < 2.5% (TCM); 3.6% (serum)Spike Recovery: 102% in Serum Storage: 2-8°CExpiration Date: 9 months from date of manufactureShipping Condition: Wet IceMaterials Provided:Pre-coated microtiter platePlate sealersWash Solution ConcentrateRhesus/Cyno Interferon Alpha 2 Standard, 10,000 pg/mlStandard DiluentSample BufferAntibody Concentrate HRP Conjugate Concentrate Concentrate DiluentTMB Substrate Stop Solution  Tech Info / Data Application Note:VeriKine Cynomolgus/Rhesus IFN-α Serum ELISA Performance EvaluationWhite Paper:Utility and Application of the VeriKine Cynomolgus/Rhesus Interferon Alpha Serum ELISADetailed Related Product Information:Quantitative Immunoassays: Single Analyte Screening Services Background:The VeriKine Cynomolgus/Rhesus IFN-alpha Serum ELISA has been developed to analyze the presence of non-human primate IFN-alpha in tissue culture media or serum samples by sandwich enzyme linked immunosorbent assay (ELISA). This kit will enable determination of IFN-alpha levels in serum, plasma and tissue culture media (TCM). The kit can measure concentrations as low as 25 pg/ml of Cynomolgus and Rhesus monkey interferon alpha in a sample. The kit is based on an ELISA with a biotinylated anti-detection antibody and a streptavidin horseradish peroxidase (HRP). Tetramethylbenzidine (TMB) is the substrate. The assay is based on PBL’s Rhesus/Cynomolgus IFNA-α2 (PBL 14110), which has been calibrated in reference to the International Standard to Human Interferon Alpha-2a. As such, it should prove an important tool in virology, immunomodulation and immunotoxicology studies conducted in non-human primates.Interferons (IFNs) are a group of cytokines which exhibit pleiotropic activities that play major roles in both innate and adaptive immunity. Type I IFNs consist of at least one IFN-β gene and protein as well as multiple IFN-α genes and proteins in most vertebrate species. IFN-α expression and secretion are primarily induced by signaling events processed through pattern recognition receptors such as the Toll-like and RIG-I like receptors (TLR and RLR, respectively). While IFN-α can be produced by most cell types, strong evidence suggests that plasmacytoid dendritic cells are a major source of IFN-α in vivo. The interferon alpha gene family is comprised of multiple distinct genes that occupy a single chromosomal locus. All sequenced vertebrate species possess a large number of distinct alpha genes suggesting a biological significance to maintaining multiple copies of IFN-alpha genes. Published reports have shown the expression patterns of the individual IFN-alpha proteins and their cell-specific function can vary suggesting unique properties of each gene in a cell-dependent manner. Following expression and secretion, IFN-α binds to a heterodimeric receptor chain consisting of IFNAR1 and IFNAR2 subunits on proximal and distal cell surfaces. Receptor binding promotes a signal transduction cascade consisting of components of the JAK-STAT signaling pathway. Hundreds of genes are regulated subsequent to binding of the IFNAR receptor subunits to IFN-α, thus leading to the antiviral, anti-proliferative and immunomodulatory activities of the cytokine. Two nonhuman primate species, Rhesus (Macaca mulatta) and Cynomolgus (Macaca fascicularis) macaques, are sufficiently genetically similar to humans that they are emerging as highly relevant animal models for studying various aspects of human physiology. For example, macaques provide valuable surrogate models for examining the pathogenicity of human viral infections such as the 1918 pandemic influenza virus. Furthermore, macaques are included in the evaluation of many new therapeutic agents aimed at modulating host immunity to either enhance or dampen immune responses. These primates also serve as important immunotoxicological models in the testing of human pharmaceuticals. Citations13 Citations:Del Prete, Gregory, et al. (2019). TLR7 agonist administration to SIV-infected macaques receiving early initiated cART does not induce plasma viremia. JCI Insight, 19 pgs. PMID: 31167974. (link)Chaudhary, Omkar, et al. (2018). Inhibition of p38MAPK in combination with ART reduces SIV-induced immune activation and provides additional protection from immune system deterioration. PLOS Pathogens, 29 pgs. PMID: 30161247. (link)Francica, Joseph, et al. (2017). Innate transcriptional effects by adjuvants on the magnitude, quality, and durability of HIV envelope responses in NHPs. Blood Advances, 14 pgs. PMID: 29296883. (link)Roques, Pierre, et al. (2017). Attenuated and vectored vaccines protect nonhuman primates against Chikungunya virus. JCI Insight, 20 pgs. PMID: 28352649. (link)Marzi, Andrea, et al. (2016). Efficacy of Vesicular Stomatitis Virus–Ebola Virus Postexposure Treatment in Rhesus Macaques Infected With Ebola Virus Makona. The Journal of Infectious Diseases, 7 pgs. PMID: 27496978. (link)Nguyen, Nguyen Van, et al. (2016). Differential Induction of Type I Interferons in Macaques by Wild-type Measles Virus or Wild-type Measles Virus with the Hemagglutinin Protein of the Edmonston Vaccine strain. Microbiology and Immunology, 5 pgs. PMID: 27278100. (link)Haberthur, Kristen, et al. (2014). Intrabronchial Infection of Rhesus Macaques with Simian Varicella Virus Results in a Robust Immune Response in the Lungs. JVI, 16 pgs. PMID: 25142604. (link)Teigler, Jeffrey (2014). Differential Innate Immune Stimulation Elicited by Adenovirus and Poxvirus Vaccine Vectors. Harvard University, 161 pgs. PMID: no PMID. (link)Meyer, Christine, et al. (2013). Bacterial artificial chromosome derived simian varicella virus is pathogenic in vivo. Virology, 12 pgs. PMID: 24010815. (link)Schramm, Lynnise, et al. (2012). High-Throughput Quantitative Real-Time Polymerase Chain Reaction Array for Absolute and Relative Quantification of Rhesus Macaque Types I, II, and III Interferon and Their Subtypes. Journal of Interferon & Cytokine Research, 9 pgs. PMID: 22817480. (link)Teigler, et al. (2012). Vaccination with Adenovirus Serotypes 35, 26, and 48 Elicits Higher Levels of Innate Cytokine Responses Than Adenovirus Serotype 5 in Rhesus Monkeys. JVI, 8 pgs. PMID: 22787208. (link)Nan, et al. (2012). Induction of Type I Interferons by a Novel Porcine Reproductive and Respiratory Syndrome Virus Isolate. Virology, 9 pgs. PMID: 22704065. (link)Zaidi, Mohammad (2011). Decreased Proviral DNA Loads and Changes in Innate Immune System-Related Transcription Factor Expression in Simian Immunodeficiency Virus-Infected Rhesus Macaques after Blocking the a4b7 Gut-Homing Integrin. Emory University, 62 pgs. PMID: no PMID. (no link)References: (For additional references, please refer to the protocol.)Krause et al. (2005). Pharma. Ther., Vol. 106 (3):299-346.Easlick et al. (2010). J. Acquir Immune Defic Syndr. 55(1):14-28.Puig et al. (2012). J. Leukoc. Biol.  91(1):147-58. Staehelin et al. (1981). Methods in Enzymology, Vol. 79 (Pestka, ed.), Academic Press, New York, 589-595. Documentation Product Notice for Standard Stock Change 46100 Protocol 46100 Certificate of Analysis (CoA) Product Flyer SDS Spike Recovery of Cyno/Rhesus IFN Alpha in serum, plasma, TCM and sample buffer using PBL"s Cynomolgus/Rhesus IFN Alpha ELISA (46100)  Three concentrations of Cynomolgus/Rhesus IFN-Alpha were added to serum, plasma (Lithium Heparin, Sodium Citrate, Sodium EDTA), TCM containing 10% FBS, and kit sample buffer. IFN-Alpha was measurable with an accuracy of +/- 20% of the expected value.

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