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covalab/Prostate Transglutaminase (TG4) (hu)/100 ug/opr0031

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DatasheetProduct descriptionPublications & ImagesRelated productsProduct Codeopr0031Uniprot IDP49221NameTransglutaminase-4, TGase-4Print datasheetAppearanceWhite lyophilized solidConstituentsThe TG is lyophilized from 10 mM Tris-HCl pH 8.1, 150 mM NaCl, 5mM DTT, 1mM EDTASample contains maltodextrinStorageStore working aliquots at a minimum of -20°CAvoid repeated freezing and thawingDelivery at ambient temperature is possibleDescriptionHis6-rhTG4 is based on clone IMAGp958A10818Q2. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAEELLL…. His6-rhTG4 is produced in E. coli and purified by ion metal chelating chromatography to more than 95 % purity.Produced inEscherichia coliSpeciesHumanFormRecombinantGene ID7047LabellingHis-TagPreparation methodAdd 10 mM Ca2+ to activate His6-rhTG4.Molecular weight78 kDaPrincipleHis6-rhTG4 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG4 may also be used for immunoprecipitation.Purity> 95 %RecommendationsAdd the volume of H2O to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.Product typeProteinsApplicationsImmunoprecipitation, Functional AssayResearch areasTransglutaminasePrint datasheet