首页 > Creative biomart > Creative biomart/Acidic Sphingomyelinase Fluorimetric Assay Kit/200 tests/Kit-0041

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Creative biomart/Acidic Sphingomyelinase Fluorimetric Assay Kit/200 tests/Kit-0041

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Kit-0041

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SpecificationRelated ProductsCat.No. :Kit-0041Product Overview :Acidic Sphingomyelinase Assay Kit (Fluorimetric) provides one of the most sensitive methods for detecting acidic SMase activity or screening its inhibitors. The kit uses AbRed as a fluorogenic probe to indirectly quantify the phosphocholine produced from the hydrolysis of sphingomyelin (SM) by sphingomyelinase (SMase). The fluorescence intensity of AbRed is proportional to the formation of phosphocholine, therefore to the SMase activity. It can be used for measuring the SMase activity in blood, cell extracts or other solutions. The kit is an optimized "mix and read" assay which is compatible with HTS liquid handling instruments.Size :200 testsApplications :Functional Studies more details.Storage :Store at -20°C.Products Types◆ Assay kits Kit-2193Alkaline Sphingomyelinase Activity Assay Kit (Colorimetric)+Inquiry Kit-2278Sphingomyelinase Activity Colorimetric Assay Kit+Inquiry See All SMase Assay kits

Creative BioMart包含动植物来源的组织裂解液的即用型面板。它们是通过在裂解缓冲液（50 mM Tris-HCl pH7.4、150 mM NaCl，1 mM PMSF，1 mM EDTA，5μg/ ml抑肽酶，5μg/ ml亮肽素，1％Triton x-100、1）中匀浆制备的％DOC，0.1％SDS）或含有5％β-巯基乙醇的改良RIPA缓冲液。Creative BioMart裂解物可用于广泛的应用，例如SDS-PAGE，免疫沉淀（IP），ELISA和Western blot分析的标准或对照。