首页 > enogene > enogene/EGFP-rProtein A/50μg/50μl/E1GA1-005

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enogene/EGFP-rProtein A/50μg/50μl/E1GA1-005

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E1GA1-005

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enogene

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4000-520-616

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EGFP-rProtein APDFCatalog Number:E1GA1-005Amount:50μg/50μlBackground:The unique, high quality recombinant EGFP-rProtein A are produced in E. coli, and purified using multiple chromatographic steps (not affinity purified with human IgG). EGFP-rProtein A bind to most mammalian immunoglobulins in their Fc binding domains and therefore are ideal for immunofluorescence staining. The genetically engineered protein was expressed and purified from transformed E. coli containing only the IgG binding regions and have 6X His-tag on their N-terminus. Other domains such as cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from all these proteins to ensure the maximum specific IgG binding and maximal fluorescence.Form of Antibody:Liquid. The product is 1 mg/ml in 10 mM Phosphate buffered saline, pH 7.4 containing, 10% glycerol and 0.1% sodium azide as preservative.Source:Recombinant expressed in E. coliPurity:>98% by SDS-PAGE and HPLCEndotoxin:< 0.05="">IgG Binding:>95%Bioburden:No organisms detectedSpecificity/Sensitivity:Under optimal conditions, 1 mg EGFP-rProtein A will bind approximately 5 mg IgG. Optimal binding of EGFP-rProtein A to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.Applications:The protein is suitable as control reagent for EGFP expression studies or as labeling reagent. Applications include: Flow cytometers, fluorescence microscopy, and microinjection, antibody purification, etc. at 1:100 dilution. Maximum absorption wavelength at 488 nm and emission wavelength at 507 nm.Storage/Stability:Store at -20°C. -80°C for long-term storage. Avoid exposure to light. Avoid repeated freezing and thawing.293T cells were stained with mouse anti-beta-tubulin antibody (E1C601) and detected with EGFP-rProtein A (Green).Price:60$

enogene的β-actin小鼠单克隆抗体（A02）PDF

目录编号：E12-045

量：100微克/ 100微升

克隆编号：A02

背景：β-肌动蛋白是已鉴定出的六种不同的肌动蛋白同工型之一。在各种物种和组织的细胞中发现的肌动蛋白分子在免疫学和物理特性方面趋于非常相似。因此，针对β-肌动蛋白的抗体可用作蛋白质印迹的上样对照。但是，应注意，β-肌动蛋白的水平在某些细胞中可能不稳定。例如，β-肌动蛋白在脂肪组织中的表达非常低，因此不应将β-肌动蛋白用作这些组织的负荷控制。

抗体形式：pH 7.4、150mM NaCl，0.02％叠氮化钠和50％甘油的磷酸盐缓冲盐水（不含Mg2 +和Ca2 +）中的小鼠IgG1。

储存/稳定性：-20℃/ 1年保存

特异性/敏感性：抗体可以检测内源性β-肌动蛋白。

反应性：H，R，M，Mk，Dg，Ch，Hm，Rb，Pg，Sh

应用范围：WB：1：5,000-10,000 IHC：1：200 IF：1：200