OverviewMag-Bind® TotalPure NGS offers an easy-to-use, reliable solution for the purification of both DNA and RNA for next-generation sequencing workflows with high recovery rates. Mag-Bind® TotalPure NGS is capable of selectively binding fragments depending on the reagent-to-sample ratio used, giving the user flexibility to perform left, right or double-sided size selection. This product is designed for both manual and fully automated purification of DNA and RNA samples, as well as for the purification of PCR products. The system combines Omega Bio-tek’s proprietary chemistries with reversible nucleic acid-binding properties of magnetic beads to selectively bind fragments and eliminate excess nucleotides, primers, and small, non-targeted products such as primer-dimers. Purified DNA and RNA is suitable for a variety of downstream applications such as NGS library preparation, microarrays, automated fluorescent sequencing and restriction enzyme digestion.No protocol change against major competitorDouble-sided size selectionDNA Clean-Up: PCR Clean-UpRNA Clean-Up: cDNA or RNA purificationManual or adaptable to most open-ended liquid handlersSignificant cost savings96- or 384-well formatsProtocols are available for the following automated platforms:Hamilton Microlab® STARHamilton Microlab® NIMBUSKingFisher™, BioSprint®, and MagMAX® 96SpecificationsFor Research Use Only. Not for use in diagnostic procedures.FeaturesSpecificationsDownstream applicationCloning, NGS, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern BlottingElution volume15 µL or aboveStarting materialDNA or RNA: PCR products, gDNA, cDNAStarting amountScalableDNA recovered>90% recovery for DNA >100 bpProcessing modeAutomated; manualThroughput96-384 samples per runDNA binding technologyMagnetic beadsStorage2°C - 8°CSpecial noteSize selection by varying beads ratioProtocol and ResourcesProduct Documentation & LiteraturePROTOCOLM1378 Mag-Bind TotalPure NGSQUICK GUIDEM1378 Mag-Bind TotalPure NGSSDSM1378 SDSSALES SHEETREFERENCEUniversity of Oregon Evaluation of Mag-Bind TotalPure NGS for DNA Size SelectionAPPLICATION NOTEAutomated DNA Cleanup for PCR and NGS Workflows: Mag-Bind® TotalPure NGS on Tecan Fluent® 780 WorkstationProduct Data#gap-671308607{padding-top:30px}Mag-Bind TotalPure NGS from Omega Bio-tek performs excellent double-side size selection. Figure 1.  Electropherogram overlay of the double-sided size selection on sheared gDNA at 0.8x/0.7x ratio set using Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable kit from Company A following manufacturer’s recommended protocols. The DNA was eluted in 25 µL and analyzed on Agilent’s TapeStation® 2200.#gap-1786873497{padding-top:30px}Mag-Bind TotalPure NGS from Omega Bio-tek has excellent recovery of targeted DNA fragments. Figure 2.  10 µL of 50 bp ladder was purified with Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable product from Company A following manufacturer’s recommended protocols. The DNA was eluted in 20 µL and analyzed on Agilent’s TapeStation® 2200.#gap-1142251980{padding-top:30px}Mag-Bind TotalPure NGS from Omega Bio-tek performs excellent RNA clean-up in a wide concentration range. Figure 3.  10 µL of RNA at 50 ng/µL and 5 ng/µL was cleaned up with Omega Bio-tek’s Mag-Bind® TotalPure NGS following manufacturer’s recommended protocols. The RNA was eluted in 20 µL and analyzed on Agilent’s TapeStation® 2200. Recovery rates were 85-92% respectively.#gap-37529430{padding-top:30px}Mag-Bind TotalPure NGS from Omega Bio-tek performs excellent double-sided size selection on DNA from NGS library prep. Figure 4.  Next-generation sequencing libraries prepared from 350 ng sheared genomic DNA using Kapa Biosystem’s HyperPrep Kits (KK8504) and Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable product from Company A on the Hamilton Microlab® STAR™. Mag-Bind® TotalPure NGS was used for 2 clean up step (0.8x and 1.0x) following Kapa Biosystems’ recommended protocol for clean up. DNA was analyzed on Agilent’s TapeStation® 2200 following library construction.#gap-496406804{padding-top:30px}CitationsView CitationsBao, Hong, et al. “Construction of a DNA Vaccine Based on the Mycobacterium Tuberculosis Ag85A/MPT64 Fusion Gene and Evaluation of Its Immunogenicity.” Molecular Medicine Reports, vol. 6, no. 6, 30 Nov. 2012, pp. 1375–1378, www.spandidos-publications.com/mmr/6/6/1375?text=fulltext, 10.3892/mmr.2012.1109. Accessed 1 June 2020.Chu, Xin, et al. “Characterization of UDP-Glucose Dehydrogenase from Pasteurella Multocida CVCC 408 and Its Application in Hyaluronic Acid Biosynthesis.” Enzyme and Microbial Technology, vol. 85, 1 Apr. 2016, pp. 64–70, www.sciencedirect.com/science/article/pii/S0141022915300946?casa_token=RbXXff7WCJMAAAAA:AR_jG3Ox_yViS0rAsOqanFCcDgEkO5sZQF6bDxTxCpJzOpkYBM9LKiqayq8VG_7zTfFgGN78hc4, 10.1016/j.enzmictec.2015.12.009. Accessed 1 June 2020.Dong, Xingyu, et al. “Downregulation of HTATIP2 Expression Is Associated with Promoter Methylation and Poor Prognosis in Glioma.” Experimental and Molecular Pathology, vol. 98, no. 2, 1 Apr. 2015, pp. 192–199, www.sciencedirect.com/science/article/pii/S0014480015000155?casa_token=CIVGtzRE8-sAAAAA:uAqLbIzZkMrpl49dlRDOp3mTtaZMsyZ-44UEUSEXdKCfVuuWpBWJRSIZ2aW0FKl3slalsPfcs00, 10.1016/j.yexmp.2015.01.013. Accessed 1 June 2020.Gao, Xue, et al. “Genomic Study of Polyhydroxyalkanoates Producing Aeromonas Hydrophila 4AK4.” Applied Microbiology and Biotechnology, vol. 97, no. 20, 3 Sept. 2013, pp. 9099–9109, 10.1007/s00253-013-5189-y. Accessed 1 June 2020.Hao, Haibang, et al. “Complete mitochondrial genome of a new vole Proedromys liangshanensis (Rodentia: Cricetidae) and phylogenetic analysis with related species: Are there implications for the validity of the genus Proedromys?.” Mitochondrial DNA 22.1-2 (2011): 28-34.Huang, Qichao, et al. “RNA-Seq Analyses Generate Comprehensive Transcriptomic Landscape and Reveal Complex Transcript Patterns in Hepatocellular Carcinoma.” PLoS ONE, vol. 6, no. 10, 17 Oct. 2011, www.ncbi.nlm.nih.gov/pmc/articles/PMC3197143/, 10.1371/journal.pone.0026168. Accessed 1 June 2020.Jiang, Han-Peng, et al. “Determination of Formylated DNA and RNA by Chemical Labeling Combined with Mass Spectrometry Analysis.” Analytica Chimica Acta, vol. 981, 15 Aug. 2017, pp. 1–10, www.sciencedirect.com/science/article/pii/S0003267017307122?casa_token=xt2DarDWL_QAAAAA:VNeMkTQYF8rqKrXCSIozXzlMQPcKL7OifWYX3AXJvn7yDqT_jGdgwyuNbs1IEJTr5l0d2iWobNs, 10.1016/j.aca.2017.06.009. Accessed 1 June 2020.Li, Haiyu, et al. “Bmi-1 Regulates Epithelial-to-Mesenchymal Transition to Promote Migration and Invasion of Breast Cancer Cells.” International Journal of Clinical and Experimental Pathology, vol. 7, no. 6, 15 May 2014, pp. 3057–3064, www.ncbi.nlm.nih.gov/pmc/articles/PMC4097277/. Accessed 1 June 2020.Li, J.M., et al. “Genetic Mechanism Associated with Congenital Cytomegalovirus Infection and Analysis of Effects of the Infection on Pregnancy Outcome.” Genetics and Molecular Research, vol. 14, no. 4, 2015, pp. 13247–13257, www.geneticsmr.com/year2015/vol14-4/pdf/gmr6714.pdf, 10.4238/2015.october.26.21. Accessed 1 June 2020.Liu, Feiwei, et al. “Taxonomic status of Tetraophasis obscurus and Tetraophasis szechenyii (Aves: Galliformes: Phasianidae) based on the complete mitochondrial genome.” Zoological science 31.3 (2014): 160-167.Li, Yuan-Yuan, et al. “Protocols for Establishing Fungi-Protocorm Culture.” Springer Protocols Handbooks, 2018, pp. 61–69, 10.1007/978-1-4939-7771-0_3. Accessed 1 June 2020.Noor‐Mohammadi, Samaneh, Azadeh Pourmir, and Tyler W. Johannes. “Method to assemble and integrate biochemical pathways into the chloroplast genome of Chlamydomonas reinhardtii.” Biotechnology and bioengineering 109.11 (2012): 2896-2903.Pimentel, Tânia, et al. “Bacterial Communities 16S RDNA Fingerprinting as a Potential Tracing Tool for Cultured Seabass Dicentrarchus Labrax.” Scientific Reports, vol. 7, no. 1, 19 Sept. 2017, pp. 1–10, www.nature.com/articles/s41598-017-11552-y, 10.1038/s41598-017-11552-y. Accessed 1 June 2020.Yue, Hao, et al. “Two Novel Mitogenomes of Dipodidae Species and Phylogeny of Rodentia Inferred from the Complete Mitogenomes.” Biochemical Systematics and Ecology, vol. 60, 1 June 2015, pp. 123–130, www.sciencedirect.com/science/article/pii/S0305197815000897?casa_token=8xflC0yV3lEAAAAA:QJ6c9zEmHvBRh6JCVbY7V47tkiOVYdJhi9NCUrRaYYF7uQwIkBqrDeUIJhgXtLWPmAbJ8-EFk6Y, 10.1016/j.bse.2015.04.013. Accessed 1 June 2020.Zhang, Feng, et al. “Genome-Wide Copy Number Variation Study and Gene Expression Analysis Identify ABI3BP as a Susceptibility Gene for Kashin–Beck Disease.” Human Genetics, vol. 133, no. 6, 21 Jan. 2014, pp. 793–799, 10.1007/s00439-014-1418-4. Accessed 1 June 2020.‌FormatMagnetic beadsSizeFREE SAMPLE, 5 mL, 50 mL, 500 mL