OverviewThe E.Z.N.A.® Gel Extraction kit uses the proprietary HiBind® spin-column technology to purify DNA fragments ranging from 100 bp to 10 kb from all grades of agarose gels with high recovery (> 80%). The kit uses a specialized binding buffer system that not only dissolves the gel slice and binds to the spin column but also includes a pH indicator for a visual representation of optimal pH for DNA binding. The bind step is followed by three rapid wash steps and DNA is eluted with deionized water or elution buffer. Purified DNA is ready for a variety of downstream applications such as ligations, PCR amplification, restriction enzyme digestion, cloning, and various labeling reactions.Rapid – DNA recovery from an agarose gel in 15 minSpecialized buffer system – Visual determination of optimum DNA binding for higher yieldsSafe – No phenol/chloroform extractionsVersatile – Spin and vacuum formats availableHigh-quality – DNA is suitable for a variety of downstream applicationsSpecificationsFor Research Use Only. Not for use in diagnostic procedures.FeaturesSpecificationsDownstream applicationCloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern BlottingElution volume30-50 µLStarting materialAgrose gel sliceStarting amountUp to 25 µg DNADNA recovered>90% recovery, 70 bp to 20 kbProcessing modeManual, centrifugation/vacuumThroughput1-12DNA binding technologySilica mini spin columnBinding capacity25 μgProcessing timeKit ComponentsItemAvailable SeparatelyHiBind® DNA Mini ColumnsView Product2 mL Collection TubesView ProductBinding Buffer (XP2)View ProductElution BufferView ProductSPW Wash BufferView ProductProtocol and ResourcesProduct Documentation & LiteraturePROTOCOLD2500 Gel Extraction KitSDSD2500 SDSSALES SHEETProduct Data#gap-1223998584{padding-top:30px}Electrophoretic Analysis of recovered DNA from an agarose gel compared to the control Figure 1.  DNA ladder was run on a 2% agarose gel and 4 different fragment sizes (200 bp, 500 bp, 1 kb, and 5 kb) were recovered using Omega Bio-tek’s E.Z.N.A.® Gel Extraction Kit and a comparable kit from Company Q following manufacturer’s recommended protocols. DNA was analyzed on a 2% TBE agarose gel with the respective companies eluate being compared to the original amount used in the gel extraction procedure. M: size marker; C: control; O: Omega Bio-tek; Q: company Q. #gap-1271254396{padding-top:30px}Comparison of DNA fragment recovery from an agarose gel Figure 2.  DNA was purified from an agarose gel using Omega Bio-tek’s E.Z.N.A.® Gel Extraction Kit and a comparable kit from Company Q. The concentration of the recovered DNA was determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system. The purified DNA normalized to input amount is shown above.#gap-208014293{padding-top:30px}CitationsView CitationsZhao, W., Wang, J., Deng, R., & Wang, X. (2008). Scale-up fermentation of recombinant Candida rugosa lipase expressed in Pichia pastoris using the GAP promoter. Journal of industrial microbiology & biotechnology, 35(3), 189-195.Li, C. J., Sun, H. W., Zhu, F. L., Chen, L., Rong, Y. Y., Zhang, Y., & Zhang, M. (2007). Local adiponectin treatment reduces atherosclerotic plaque size in rabbits. Journal of Endocrinology, 193(1), 137-145.Ye, X., Li, J., Lu, M., Deng, G., Jiang, X., Tian, Y., … & Jian, Q. (2011). Identification and molecular typing of Streptococcus agalactiae isolated from pond-cultured tilapia in China. Fisheries Science, 77(4), 623-632.Laurila, J., Ahola, V., Lehtinen, A., Joutsjoki, T., Hannukkala, A., Rahkonen, A., & Pirhonen, M. (2008). Characterization of Dickeya strains isolated from potato and river water samples in Finland. European Journal of Plant Pathology, 122(2), 213-225.Laurila, J., Ahola, V., Lehtinen, A., Joutsjoki, T., Hannukkala, A., Rahkonen, A., & Pirhonen, M. (2008). Characterization of Dickeya strains isolated from potato and river water samples in Finland. European Journal of Plant Pathology, 122(2), 213-225.Jiang, Y., Li, Y., Fang, S., Jiang, B., Qin, C., Xie, P., … & Li, G. (2014). The role of MALAT1 correlates with HPV in cervical cancer. Oncology letters, 7(6), 2135-2141.Xu, C., Zhao, J., Hu, X., & Zhang, G. (2007). Isolation and identification of four infectious bronchitis virus strains in China and analyses of their S1 glycoprotein gene. Veterinary microbiology, 122(1-2), 61-71.Koenig, C., Hirsh, A., Bucks, S., Klinner, C., Vogel, H., Shukla, A., … & Grosse-Wilde, E. (2015). A reference gene set for chemosensory receptor genes of Manduca sexta. Insect biochemistry and molecular biology, 66, 51-63.Shu, Y., Du, Y., & Wang, J. (2011). Molecular characterization and expression patterns of Spodoptera litura heat shock protein 70/90, and their response to zinc stress. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 158(1), 102-110.Du, R., Du, R., Chen, Y. F., An, X. R., Yang, X. Y., Ma, Y., … & Qin, J. (2005). Cloning and sequence analysis of myostatin promoter in sheep: Full Length Research Paper. DNA Sequence, 16(6), 412-417.Cai, L., Ni, J., Xia, Y., Zi, Z., Ning, K., Qiu, P., … & Yu, X. (2012). Identification of an emerging recombinant cluster in porcine circovirus type 2. Virus research, 165(1), 95-102.Xuan, K., Jin, F., Liu, Y. L., Yuan, L. T., Wen, L. Y., Yang, F. S., … & Jin, Y. (2008). Identification of a novel missense mutation of MSX1 gene in Chinese family with autosomal-dominant oligodontia. Archives of oral biology, 53(8), 773-779.Chen, R., Guo, L., & Dang, H. (2011). Gene cloning, expression and characterization of a cold-adapted lipase from a psychrophilic deep-sea bacterium Psychrobacter sp. C18. World Journal of Microbiology and Biotechnology, 27(2), 431-441.Liu, J. M., Xin, X. J., Li, C. X., Xu, J. H., & Bao, J. (2012). Cloning of thermostable cellulase genes of Clostridium thermocellum and their secretive expression in Bacillus subtilis. Applied biochemistry and biotechnology, 166(3), 652-662.Farnelid, H., Bentzon-Tilia, M., Andersson, A. F., Bertilsson, S., Jost, G., Labrenz, M., … & Riemann, L. (2013). Active nitrogen-fixing heterotrophic bacteria at and below the chemocline of the central Baltic Sea. The ISME journal, 7(7), 1413.Fu, X., Li, N., Liu, L., Lin, Q., Wang, F., Lai, Y., … & Wu, S. (2011). Genotype and host range analysis of infectious spleen and kidney necrosis virus (ISKNV). Virus genes, 42(1), 97-109.X. Guo, X. Xia, R. Tang, J. Zhou, H. Zhao, K. 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