OverviewThe E.Z.N.A.® Plasmid Maxi Kit utilizes the convenient spin-column format to deliver high-quality plasmid DNA following a simple “bind-wash-elute” procedure without need for expensive accessories. The yields vary according to plasmid copy number, E. coli strain, and growth conditions. 50-200 mL of bacterial cultures in LB medium typically produces 0.6-1.2 mg of high copy number plasmid DNA. Up to 500 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR, in vitro transcription, transformation, and other applications.Speed – Purification of plasmid DNA in < 60 minutesSafety – No phenol/chloroform extractionsVersatile – Spin and vacuum formats availableHigh quality – DNA is suitable for a variety of downstream applicationsSpecificationsFor Research Use Only. Not for use in diagnostic procedures.FeaturesSpecificationsDownstream ApplicationCloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.Starting material 50-200 mL LB culture with OD600 between 2 and 3; or equivalentPlasmid typeHigh-copy, low-copy, cosmid DNAProcessing modeManual (centrifugation or vacuum)Throughput1 - 24DNA binding technologySilica Maxi Spin ColumnLysate clearance methodCentrifugationProcessing timeYield600-1200 µg for high copy-number; 50-300 µg for low copy-numberKit ComponentsItemAvailable SeparatelyHiBind® DNA Mini ColumnsView Product50 mL Collection Tubes---Solution IView ProductSolution IIView ProductSolution IIIView ProductHBC BufferView ProductDNA Wash BufferView ProductRNase AView ProductElution BufferView ProductProtocol and ResourcesProduct Documentation & LiteraturePROTOCOLD6922 Plasmid DNA Maxi KitSDSD6922 SDSSALES SHEETProduct Data#gap-467020453{padding-top:30px}Yield and quality of plasmid DNA purified using E.Z.N.A.® Plasmid Midi and Maxi Kits Figure 1.  pGEM plasmid was purified from 250 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Plasmid Maxi Kit following manufacturer’s recommended protocols. Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system.#gap-1105355186{padding-top:30px}CitationsView CitationsChecco, James W., et al. “Aplysia Allatotropin-Related Peptide and Its Newly Identified d-Amino Acid–Containing Epimer Both Activate a Receptor and a Neuronal Target.” Journal of Biological Chemistry, vol. 293, no. 43, 26 Oct. 2018, pp. 16862–16873, www.jbc.org/content/293/43/16862.short, 10.1074/jbc.RA118.004367. Accessed 1 June 2020.Tan, Xiao, and Samuel G. Awuah. “A Cell-Based Screening System for RNA Polymerase I Inhibitors.” MedChemComm, vol. 10, no. 10, 16 Oct. 2019, pp. 1765–1774, pubs.rsc.org/en/content/articlehtml/2019/md/c9md00227h, 10.1039/C9MD00227H. Accessed 1 June 2020.Zheng, Fang, et al. “Chitosan Nanoparticle as Gene Therapy Vector via Gastrointestinal Mucosa Administration: Results of an in Vitro and in Vivo Study.” Life Sciences, vol. 80, no. 4, 2 Jan. 2007, pp. 388–396, www.sciencedirect.com/science/article/pii/S0024320506007363?casa_token=zBzVOepd1NkAAAAA:uOnGysv24gs4oWwmkzcB_Cs_9aqgzhFkTGkUmgWOR6ZGh3tl8ZKv6-PvzRfKghxTEmaxspU7-YM, 10.1016/j.lfs.2006.09.040. Accessed 1 June 2020.SizeFREE SAMPLE, 2 preps, 20 preps, 100 prepsFormatMaxiprep