提醒:代购产品,无质量问题不接受退换货,下单前请仔细核对信息。下单后请及时联系客服 核对商品价格,订单生效后再付款。
Biotium/NucView 488 and RedDot 2 Apoptosis and Necrosis Kit/30072/100-assays
价格:

自营商城

解放采购

正品保障

及时交付

厂家直采

一站服务

货号:
品牌:
会员服务:
尊享会员价
贵宾专线
运费优惠
闪电退款
福利优惠
上门换新
友情提示
以上价格仅为参考,请联系客服询价。
免费咨询热线
4000-520-616
产品说明
NucView™488andRedDot™2Apoptosis/NecrosisAssayKitcontainsNucView™488Caspase-3Substratefordetectionofcaspase-3/7activity.Thefar-reddead-cellstainRedDot™2isincludedforstainingnecroticandlateapoptoticcellsthathavecompromisedplasmamembraneintegrity.NucView™488andRedDot™2Apoptosis/NecrosisAssayKitcontainsNucView™488Caspase-3Substratefordetectionofcaspase-3/7activity.Thefar-reddead-cellstainRedDot™2isincludedforstainingnecroticandlateapoptoticcellsthathavecompromisedplasmamembraneintegrity.Thiskitprovidesaconvenienttoolforprofilingapoptoticandnecroticcellpopulationsbyfluorescencemicroscopyorflowcytometry.Incontrasttootherfluorogeniccaspasesubstratesorfluorescentcaspaseinhibitorbased(FLICA)assays,NucView™488Caspase-3Substratecanbeusedtodetectcaspase-3/7activitywithinindividualintactcellswithoutinhibitingapoptosisprogression.ThesubstrateconsistsofafluorogenicDNAdyecoupledtothecaspase-3/7DEVDrecognitionsequence.Thesubstrate,whichisinitiallynon-fluorescent,penetratestheplasmamembraneandentersthecytoplasm.Inapoptoticcells,caspase-3/7cleavesthesubstrate,releasingthehigh-affinityDNAdye,whichmigratestothecellnucleusandstainsDNAwithbrightgreenfluorescence.Thus,NucView™488Caspase-3Substrateallowsdetectioncaspase-3/7activityandvisualizationofmorphologicalchangesinthenucleusduringapoptosis.RedDot™2isacellmembrane-impermeable,far-reddyewithhighselectivityformembranecompromisedordeadcells.RedDot™2hasfar-redemissionat695nm,well-separatedfromthegreenfluorescenceofNucView™488. TheexcitationmaximumofRedDot™2is665nm,butitcanbeefficientlyexcitedbywavelengthsfrom488to647nm,andthereforecanbeusedwiththe488nmflowcytometrylaserline.Note:whileNucView™488stainingisformaldehyde-fixableandcompatIBLewithsubsequentimmunostaining,fixationafterstainingwithRedDot™2isnotrecommendedbecauseitcanresultinincreasedbackgroundstainingofhealthycells.SeeourfullselectionofNucView™substratesandkits.NucView™enzymesubstratetechnologyiscoveredbyU.S.patents. ReferencesCen,etal.DEVD-NucView488:anovelclassofenzymesubstratesforreal-timedetectionofcaspase-3activityinlivecells.TheFASEBJournalpublishedonlineFebruary8,2008.Downloadafulllistofreferences:ValidatedcelllinesforNucView488withreferences

EB(溴化乙锭):溴化乙锭是一种高度灵敏的荧光染色剂,用于观察琼脂糖聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号,可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。

 

SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下,SYBR Green I发出微弱的荧光,但一旦与双链DNA结合后,荧光大大增强。因此,SYBR Green I的荧光信号强度与双链DNA的数量相关,可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm,发射波长最大约为520nm