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Biotium/AccuBlue Broad Range dsDNA Standards, set of nine/31007C/0-5-ml-each
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Stand-alone set of the nine double-stranded DNA standards provided with the AccuBlue™ Broad Range dsDNA Quantitation Kit (31007). Calf thymus dsDNA in 10 mM Tris pH 7.5, 1 mM EDTA, 2 mM sodium azide at 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL, 0.5 mL each. Stand-alone set of the nine double-stranded DNA standards provided with the AccuBlue™ Broad Range dsDNA Quantitation Kit (31007). Calf thymus dsDNA in 10 mM Tris pH 7.5, 1 mM EDTA, 2 mM sodium azide at 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL, 0.5 mL each. Other DNA Quantitation Kits: dsDNA Quantitation KitLinear detection range (dsDNA in assay)Ex/Em (nm)Features AccuBlue™ NextGen1 pg*-3 ng468/507Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity. AccuClear™ Ultra High Sensitivity30 pg-250 ng468/507This kit offers great sensitivity and the widest linear range. AccuBlue™ High Sensitivity0.2-100 ng485/530Quantitation of dsDNA from 0.2-100 ng, with a safer, membrane impermeable DNA dye. AccuGreen™ High Sensitivity0.1-100 ng502/523Quantitation of DNA using the Qubit® fluorometer from Thermo Fisher. AccuBlue™ Broad Range2-2000 ng350/460For quantitation of high concentration DNA in a broad linear range with blue fluorescence. Assay can be extended to 4000 ng with minor loss of linearity. References 1. PLoS ONE (2013), doi:10.1371/journal.pone.0080613 2. J Thromb Thrombolytic (2014), doi: 10.1007/s11239-014-1137-7 3. Molecular Ecology (2015), doi: 10.1111/mec.13167

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SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下,SYBR Green I发出微弱的荧光,但一旦与双链DNA结合后,荧光大大增强。因此,SYBR Green I的荧光信号强度与双链DNA的数量相关,可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm,发射波长最大约为520nm