产品说明
Stand-alone set of the nine double-stranded DNA standards provided with the AccuBlue™ Broad Range dsDNA Quantitation Kit (31007). Calf thymus dsDNA in 10 mM Tris pH 7.5, 1 mM EDTA, 2 mM sodium azide at 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL, 0.5 mL each.
Stand-alone set of the nine double-stranded DNA standards provided with the AccuBlue™ Broad Range dsDNA Quantitation Kit (31007). Calf thymus dsDNA in 10 mM Tris pH 7.5, 1 mM EDTA, 2 mM sodium azide at 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL, 0.5 mL each.
Other DNA Quantitation Kits:
dsDNA Quantitation KitLinear detection range (dsDNA in assay)Ex/Em (nm)Features
AccuBlue NextGen1 pg*-3 ng468/507Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity.
AccuClear Ultra High Sensitivity30 pg-250 ng468/507This kit offers great sensitivity and the widest linear range.
AccuBlue High Sensitivity0.2-100 ng485/530Quantitation of dsDNA from 0.2-100 ng, with a safer, membrane impermeable DNA dye.
AccuGreen High Sensitivity0.1-100 ng502/523Quantitation of DNA using the Qubit® fluorometer from Thermo Fisher.
AccuBlue Broad Range2-2000 ng350/460For quantitation of high concentration DNA in a broad linear range with blue fluorescence. Assay can be extended to 4000 ng with minor loss of linearity.
References
1. PLoS ONE (2013), doi:10.1371/journal.pone.0080613
2. J Thromb Thrombolytic (2014), doi: 10.1007/s11239-014-1137-7
3. Molecular Ecology (2015), doi: 10.1111/mec.13167
★EB(溴化乙锭):溴化乙锭是一种高度灵敏的荧光染色剂,用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号,可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。
★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下,SYBR Green I发出微弱的荧光,但一旦与双链DNA结合后,荧光大大增强。因此,SYBR Green I的荧光信号强度与双链DNA的数量相关,可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm,发射波长最大约为520nm。
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