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SMOBIO/[DM4160] FluoroBand™ XL 25 kb Fluorescent DNA Ladder, Broad Range (up to 25 kb), 500 μl/Broad Range (up to 25 kb), 500 μl/DM4160
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Product overview Technical/Spec Support/Documents FAQsApplication references Related products Description The DM4160 FluoroBand™ XL 25 kb Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM4160 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank. FeaturesSharp bandsQuick reference— enhanced bands Ready-to-use— premixed with loading dye for direct loadingStable— room temperature storage over 6 monthsDirectly observed by UV or blue light— premixed with high sensitive DNA fluorescent dyeSource Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.Range 250 ~ 25,000 bp Concentration 51.6 µg/ 500 µl Recommended loading volume 5 µl/ wellStorageProtected from light Room temperature for 6 months4°C for 12 months -20°C for 24 months  SpecificationCat. No.DM4160Series NameFluoroBand™Product Size500 μlSize Range250 – 25000 bpBand Number14Tracking DyeBromophenol blue and Xylene cyanol FFEnhanced Band1000 and 3000 bp ManualManual_DM4160_FluoroBand™ XL 25 kb Fluorescent DNA Ladder, Broad Range (up to 25 kb)SDSSDS_DM4160 Are the DNA markers/ladders produced by SMOBIO sufficient in quantity? Yes, all the DNA markers of SMOBIO have been passed in the QC processes including repeated optical density measurements to ensure the quantity of total DNA. Can I combine non-fluorescent markers and fluorescent loading dye (ex. DL5000) to replace FluoroBand™ fluorescent DNA ladders?SMOBIO’s fluorescent DNA ladders is better in intensity and accuracy than a fluorescent marker produced by mixing non-fluorescent one with reagents containing fluorescent DNA dyes such as DL5000. In another aspect, DL5000 is designed for quick screening, not intended for preparation of DNA ladders which needs careful calibration. Therefore we suggest using our fluorescent DNA ladders directly. Will FluoroBand™ fluorescent DNA ladders gradually lose fluorescent intensity?The fluorescent signals of a fluorescent DNA ladder might be reduced if frequently exposed to light for a long term. Therefore, we suggest keeping fluorescent DNA ladders from exposure to light. Can fluorescent DNA markers be visualized when illuminated with UV light?Yes, it is possible to view the fluorescent signals under blue light and UV light.   The conserved basic residues and the charged amino acid residues at the α-helix of the zinc finger motif regulate the nuclear transport activity of triple C2H2 zinc finger proteinsChih-Ying Lin, Lih-Yuan Lin PLoS One. 2018; 13(1): e0191971. Published online 2018 Jan 30. doi: 10.1371/journal.pone.0191971PMCID: PMC5790263Transposable elements generate population-specific insertional patterns and allelic variation in genes of wild emmer wheat (Triticum turgidum ssp. dicoccoides)Katherine Domb, Danielle Keidar, Beery Yaakov, Vadim Khasdan, Khalil Kashkush BMC Plant Biol. 2017; 17: 175. Published online 2017 Oct 27. doi: 10.1186/s12870-017-1134-zPMCID: PMC5659041FluoroBand™ DNA Ladder series ExcelBand™ DNA Ladder series FluoroDye™ DNA Fluorescent Loading DyeExcellent for premix with DNA sample Sensitivity up to 0.14 ng DNASafety dyeConvenience - monitor the electrophoresis in real-timeFluoroVue™ Nucleic Acid Gel StainExcellent for in-gel stainingSensitivity up to 0.14 ng DNA or 1 ng total RNAA safer alternative to EtBr Suitable to blue or UV light B-BOX™ Blue Light LED Epi-illuminator470 nm long wavelengthImproved cloning efficiency Compact, light-weight, and portable (less than 1 kg)Adjustable and removable filter plate allows for gel cutting, visualization, and documentation 

SMOBIO的B-BOX™是一种长波长蓝光LED落射照明器。它设计紧凑,结构坚固。B-BOX™落射照明器由于其非紫外线光源和低至12伏的低工作电压,并且为非致癌DNA /蛋白质染料提供了工作能力,因此为用户提供了前所未有的安全性。B-BOX™带有高质量的非闪烁LED灯,对DNA和蛋白质染料高度敏感。B-BOX™可以极大地减轻凝胶提取的日常琐事,即使在明亮的环境光下也可以轻松进行可视化和凝胶切割。多角度滤板(目录号VE0102)为凝胶切割,可视化和记录提供了最佳角度。B-BOX™还带有用于过滤和可视化的琥珀色滤镜(目录号VE0103)。Phox™Photobox(目录号 VE0104)为使用任何智能手机拍摄的图像提供了一个迷你暗室环境。最后,工作区域周围的内置屏障系统有助于清洁。




特征


提高克隆效率 


紧凑,轻巧和便携式(重量不足1千克)


安全功能包括:470 nm长波长,对用户没有任何紫外线辐射危害


与非致癌,非溴化乙锭的DNA染料兼容


用户友好:样品易于观察(使用滤板或护目镜时) 


LED光源持续长达50,000小时


出色的检测灵敏度:使用FluoroStain™DNA荧光染色染料时,DNA≤0.04 ng,使用FluoroStain™蛋白荧光染色染料时,蛋白≤3ng(与银染一样灵敏)


可调和可移动的滤板可进行凝胶切割,可视化和记录


内置屏障设计,易于清理


在明亮的环境光下可见


借助其自身的内置散热器,强调了最小的功率依赖,低发热


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