Product overview Technical/Spec Support/Documents Tips for usage Application references Related products DescriptionExcelTaq™ Taq DNA Polymerase is a recombinant thermo-stable Taq DNA polymerase expressed and purified from an E. coli strain carrying the cloned gene. With a high DNA synthesis rate and high thermo-stability, ExcelTaq™ Taq DNA Polymerase is suitable for common and specialized PCR applications.Features5"→3" DNA polymerase activity5"→3" exonuclease activityNo detectable 3"→5" exonuclease (proofreading) activityGenerates PCR products with 3’-dA overhangsThermo-stable – half-life  lasts for more than 40 min at 95°CApplications Routine PCR Amplification of DNA fragments up to 8 kbGeneration of PCR products for TA cloningDNA labelingStorage-20°C for 24 monthsElongation capabilityExcelTaq™ Taq DNA Polymerase can amplify PCR products from λDNA up to 15 kb (M: DM3100).  SensitivityExcelTaq™ Taq DNA Polymerase can amplify PCR products from as little as 1 pg of template DNA (M: DM3100).    ContentsComponentVolumeTaq DNA Polymerase (5 U/μl)100 μl10X Taq  Buffer2 x 1 mlStorage Buffer20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol10X Taq buffer200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgCl2, 1% Triton X-100Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. Storage-20°C for 24 months    ManualManual_TP1000_ExcelTaq™ Taq DNA PolymeraseSDSSDS_TP1000 Recommended PCR Condition Template1 – 150 ngForward primer0.1 – 0.5 µMReverse primer0.1 – 0.5 µM10X Taq Buffer5 µldNTPs0.2 mM (each)Taq DNA Polymerase0.25 µl (1.25U)H2Oto 50 µlTotal volume50 µl Recommended PCR ProgramStepsTemp.TimeCyclesTemplate denature94°C2 min1Denature94°C30 sec25-40Annealing50-68°C*30 secExtension72°C30 sec/kbFinal extension72°C1 min1*Optimal PCR condition varies according to primers’ thermodynamic properties.Mutations in the PKM2 exon-10 region are associated with reduced allostery and increased nuclear translocationTsan-Jan Chen, Hung-Jung Wang, Jai-Shin Liu, Hsin-Hung Cheng, Sheng-Chieh Hsu, Meng-Chen Wu, Chien-Hung Lu, Yu-Fang Wu, Jing-Wen Wu, Ying-Yuan Liu, Hsing-Jien Kung & Wen-Ching Wang Commun Biol. 2019; 2: 105. Published online 2019 Mar 15. doi: 10.1038/s42003-019-0343-4PMCID: PMC6420622[RP1000] ExcelRT™ Reverse TranscriptaseHigh yieldThermostable, up to 50°C, during first strand synthesisHigh processivity, generating cDNA up to 8 kbReduced RNase H ribonuclease activity[TP5000] ExcelTaq™ Hot Start II DNA PolymeraseAptamer-based hot start PCRReversible enzyme inactivationOmits extra enzyme activation stepConvenient for room temperature PCR set-upHigh yield and specificity of target ampliconsWide range of amplicon length (up to 10 kb)High sensitivity (as low as 1 fg of plasmid)[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) High StabilityFast Hot StartHigh SensitivityLow Background / High Specificity Suitable for Fast Program Smart Blue Contrast Dye [TP1200] ExcelTaq™ 5X PCR Master Dye Mix5’→3’ DNA polymerase activityNo detectable 3"→5" exonuclease (proofreading) activityGenerates PCR products with 3"-dA overhangsHigh yield PCR High reproducibilityReduced  pipetting errorsIncludes tracking dye for direct loading after PCR