Product overview Technical/Spec Support/Documents Tips for usage Application references Related products Description The ExcelTaq™ 5X/2X PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as a master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of templates and primers. This not only saves valuable time in the laboratory, but also reduces pipetting and reagent handling errors. The PCR Master Mix is supplied as a 5X/2X concentrated ready-to-use mixture of recombinant Taq DNA Polymerase, reaction buffer, MgCl2 (TP1120 contains MgSO4), dNTP, and enzyme stabilizer enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent conveniently. This product is supplied with 6X DNA Loading Dye (Blue) containing two tracking dyes (Xylene cyanol FF and Bromophenol blue) for post PCR analysis through the use of agarose gel electrophoresis.Features5’→3’ DNA polymerase activityNo detectable 3"→5" exonuclease (proofreading) activityGenerates PCR products with 3"-dA overhangsHigh yield PCR High reproducibilityReduced pipetting errorsApplications Routine PCRColony PCRHigh throughput PCRAmplification of DNA fragments up to 8 kbGeneration of PCR products for TA cloningDNA labelingStorage4°C for 6 months-20°C for 24 monthsElongation capabilityExcelTaq™ PCR Master Mix can reliably amplify λDNA up to 8 kb in length. (M: DM3100) Contents ComponentVolumeCat. No ExcelTaq™ 5X PCR Master Mix2 x1 mlTP1100 (200 Rxn) 6X DNA Loading Dye (Blue)2 x 1 mlExcelTaq™ 2X PCR Master Mix (MgSO4)2 x1.25 mlTP1120 (100 Rxn) 6X DNA Loading Dye (Blue)1 ml Storage4°C for 6 months-20°C for 24 months ManualManual_TP1100_ExcelTaq™ 5X PCR Master MixManual_TP1120_ExcelTaq™ 2X PCR Master Mix (MgSO4)SDSSDS_TP1100SDS_TP1120 Recommended PCR ConditionTemplate1 – 150 ngForward primer0.1 – 0.5 µMReverse primer0.1 – 0.5 µM5X (or 2X) PCR Master Mix10 µl (or 25 µl)H2Oto 50 µlTotal volume50 µlRecommended PCR Program StepsTemp.TimeCycles Templatedenature94°C2 min1 Denature94°C30 sec25-40 Annealing50-68°C*30 sec Extension72°C30 sec/kb Final extension72°C1 min1 *Optimal PCR condition variesaccording to primers’ thermodynamic properties.  Morphological and molecular description of Rhadinorhynchus laterospinosus Amin, Heckmann & Ha, 2011 (Acanthocephala, Rhadinorhynchidae) from marine fish off the Pacific coast of Vietnam.Amin OM, Heckmann RA, Dallarés S, Constenla M, Ha NV. Parasite. 2019;26:14. doi: 10.1051/parasite/2019015. Epub 2019 Mar 6.PMCID: PMC6402367 High Fidelity PCR amplificationAmplification of target gene with HiFi™ DNA polymerase to minimize error rate.[TF1000] SMO-HiFi™ DNA Polymerase, (1 U/μl, 100 U)[TF3000] G-HiFi™ DNA Polymerase, (1 U/μl, 100 U)Gel electrophoresisStaining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.   Safe fluorescent dyes[NS1000] FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500 μl[DS1000] FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X), 500 μl[DL5000] FluoroDye™ DNA Fluorescent Loading Dye (Green, 6X), 1 mlBlue-light illuminator[VE0100] B-BOX™ Blue Light LED Epi-illuminatorLigationBlund-end PCR amplicons can directly ligate with PCR cloning vector.   [CV1100] GetClone™ PCR Cloning Vector II, 20 RxnTransformationPrepare competent cells with high efficiency and transform with time-saving protocol.[CK1000] Champion™ E. coli Transformation Kit