产品说明
Product overview Technical/Spec Support/Documents FAQs Tips for usage Related products DescriptionThe ExcelTaq™ 5X Fluorescent PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as a master mix for virtually all PCR applications. This product is supplied as a 5X concentrated mixture containing all the essential ingredients for PCR with the exception of templates and primers. In addition, the mixture contains electrophoresis tracking dye (Bromophenol blue) and a safer fluorescent DNA staining dye, which enables the user to track the electrophoresis process in real time as well as eliminates the need for the staining process. The resultant PCR reaction mixture is sufficiently dense enough to be loaded directly into a TAE or TBE buffered gel for electrophoresis. Features5’→3’ DNA polymerase activityNo detectable 3"→5" exonuclease (proofreading) activityGenerates PCR products with 3"-dA overhangsHigh yield PCR High reproducibilityReduced pipetting errorsFor direct loading into electrophoresis analysisDNA bands can be visualized directly under UV or 470 nm blue light illuminationApplications Routine PCRColony PCRHigh throughput PCRAmplification of DNA fragments up to 8 kbGeneration of PCR products for TA cloningDNA labeling StorageProtected from light and avoid multiple freeze/thaw cycles4°C for 6 months-20°C for 24 monthsElongation capabilityλDNA was amplified with ExcelTaq™ 5X Fluorescent PCR Master Mix. The λDNA was amplified with specific primers for amplifying different ranges. The pictures were captured under B-BOX™ blue light as seen in the left picture. While using UV light, the amplicons can be seen in the right picture (M: DM 3160). Colony PCR Colony PCR with ExcelTaq™ 5X Fluorescent PCR Master Dye Mix. The JM109 or modified JM109 was used for amplifying the genomic galactosidase Z gene. The pictures were captured under B-BOX™ blue light as seen in the left picture. While using UV light, the amplicons can be seen in the right picture (M: DM 3160). ContentsComponentVolumeExcelTaq™ 5X Fluorescent PCR Master Mix2 x 1 mlStorageProtected from light and avoid multiple freeze/thaw cycles4°C for 6 months-20°C for 24 months ManualManual_TP1260_ExcelTaq™ 5X Fluorescent PCR Master Mix SDSSDS_TP1260 Why is the size of the PCR product slightly different from prediction when used with TP1260 for PCR reaction? It is due to variation of the fluorescent dye/DNA ratio. If less DNA was bond with too much fluorescent dye, the DNA would migrate slower in the electrophoresis and presented a larger size than expected. TP1260 therefore is not suitable for running such experiments needed to distinguish subtle differences. Recommended PCR ConditionTemplate1 – 150 ngForward primer0.1 – 0.5 µMReverse primer0.1 – 0.5 µM5X Fluorescence PCR Master Mix10 µlH2Oto 50 µlTotal volume50 µlRecommended PCR ProgramStepsTemp.TimeCyclesTemplate denature94°C2 min1Denature94°C30 sec25-40Annealing50-68°C*30 secExtension72°C30 sec/kbFinal extension72°C1 min1*Optimal PCR condition varies according to primers’ thermodynamic properties.B-BOX™ Blue Light LED Epi-illuminator470 nm long wavelengthImproved cloning efficiency Compact, light-weight, and portable (less than 1 kg)Adjustable and removable filter plate allows for gel cutting, visualization, and documentationFluoroBand™ DNA Ladder seriesSharp bandsQuick reference— enhanced bands Ready-to-use— premixed with loading dye for direct loadingStable— room temperature storage over 6 monthsDirectly observed by UV or blue light— premixed with high sensitive fluorescent dyeFluoroVue™ Nucleic Acid Gel StainExcellent for in-gel stainingSensitivity up to 0.14 ng (DNA) or or 1 ng (total RNA)A safe alternative to EtBrSuitable for blue or UV lightHigh Fidelity PCR amplificationAmplification of target gene with HiFi™ DNA polymerase to minimize error rate.[TF1000] SMO-HiFi™ DNA Polymerase, (1 U/μl, 100 U)[TF3000] G-HiFi™ DNA Polymerase, (1 U/μl, 100 U)Gel electrophoresisStaining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency. Safe fluorescent dyes[NS1000] FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500 μl[DS1000] FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X), 500 μl[DL5000] FluoroDye™ DNA Fluorescent Loading Dye (Green, 6X), 1 mlBlue-light illuminator[VE0100] B-BOX™ Blue Light LED Epi-illuminatorLigationBlund-end PCR amplicons can directly ligate with PCR cloning vector. [CV1000] GetClone™ PCR Cloning Vector, 20 Rxn[CV1100] GetClone™ PCR Cloning Vector II, 20 RxnTransformationPrepare competent cells with high efficiency and transform with time-saving protocol.[CK1000] Champion™ E. coli Transformation Kit
SMOBIO的B-BOX™是一种长波长蓝光LED落射照明器。它设计紧凑,结构坚固。B-BOX™落射照明器由于其非紫外线光源和低至12伏的低工作电压,并且为非致癌DNA /蛋白质染料提供了工作能力,因此为用户提供了前所未有的安全性。B-BOX™带有高质量的非闪烁LED灯,对DNA和蛋白质染料高度敏感。B-BOX™可以极大地减轻凝胶提取的日常琐事,即使在明亮的环境光下也可以轻松进行可视化和凝胶切割。多角度滤板(目录号VE0102)为凝胶切割,可视化和记录提供了最佳角度。B-BOX™还带有用于过滤和可视化的琥珀色滤镜(目录号VE0103)。Phox™Photobox(目录号 VE0104)为使用任何智能手机拍摄的图像提供了一个迷你暗室环境。最后,工作区域周围的内置屏障系统有助于清洁。
特征
提高克隆效率
紧凑,轻巧和便携式(重量不足1千克)
安全功能包括:470 nm长波长,对用户没有任何紫外线辐射危害
与非致癌,非溴化乙锭的DNA染料兼容
用户友好:样品易于观察(使用滤板或护目镜时)
LED光源持续长达50,000小时
出色的检测灵敏度:使用FluoroStain™DNA荧光染色染料时,DNA≤0.04 ng,使用FluoroStain™蛋白荧光染色染料时,蛋白≤3ng(与银染一样灵敏)
可调和可移动的滤板可进行凝胶切割,可视化和记录
内置屏障设计,易于清理
在明亮的环境光下可见
借助其自身的内置散热器,强调了最小的功率依赖,低发热
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