Product overview Technical/Spec Support/Documents Tips for usage Related products DescriptionThe ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is designed for amplifying targeted DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process. ExcelTaq™ Blood Direct PCR Master Mix Kit is a unique blend of 5X concentrated mixture containing all the essential components of a PCR reaction. The ExcelTaq™ Blood Direct PCR Master Mix Kit also contains the PCR-friendly loading and tracking dye solution Orange G. The inclusion of Orange G tracking dye allows for a more convenient post-PCR gel electrophoresis analysis. ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is capable of tolerating the presence of PCR interfering/ inhibiting substances in blood. The ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is ideal for high-throughput screening of blood samples for high reproducibility. ExcelTaq™ 5X Blood Direct DNA PCR Master Mix Kit includes a pair of Positive Control Primers (CCR5) that are compatible with primate blood samples. Features5"→3" DNA polymerase activityNo detectable 3"→5" exonuclease (proofreading) activityGenerates PCR products with 3"-dA overhangs High throughput PCRHigh yield PCR High reproducibility, with reduced pipetting errorsCompatible with most anticoagulantsApplications Direct amplification of DNA from blood samplesHigh throughput screening without DNA purification Suitable for multiplex PCRStorageCaution: Avoid Multiple Freeze/Thaw Cycles4°C for 6 months-20°C for 24 monthsCompatible with most anticoagulants ExcelTaq™ 5X Blood Direct PCR Master Mix amplified 200 bp from differently treated blood using CCR5 specific primers. (M: DM2100) Contents ComponentVolume 5X Blood Direct PCR Master Mix2 x 1 ml Positive Control Primers (10 μM,each)25 μl CCR5 control primer sequenceCCR5-F:5"-CTCCCAGGAATCATCTTTACC-3"CCR5-R:5"-TCATTTCGACACCGAAGCAG-3"StorageCaution: Avoid Multiple Freeze/Thaw Cycles4°C for 6 months-20°C for 24 months ManualManual_TP2100_ExcelTaq™ 5X Blood Direct PCR Master Mix KitSDSSDS_TP2100 Recommended PCR Condition Blood3μl Forward primer0.1– 0.5 µM Reverse primer0.1– 0.5 µM 5X Blood Direct PCR Master Mix10μl H2Oto50 μl Total volume50µl Note: Mix well before PCR Reaction Recommended PCRProgram StepsTemp.TimeCycles Templatedenature94°C3 min1 Denature94°C30 sec25-40 Annealing50-68°C**30 sec Extension72°C60 sec/kb Final extension72°C3 min1 *Optimal PCR condition variesaccording to primers’ thermodynamic properties.  Post amplificationanalysisCentrifuge PCR reaction mixture at 1000 × g for 1 ~ 3 minutes. Load only the clear supernatant of the PCRproducts for gel electrophoresis analysis. [TP2000] ExcelTaq™ Blood Direct DNA PolymeraseHigh yieldThermostable, up to 50°C, during first strand synthesisHigh processivity, generating cDNA up to 8 kbReduced RNase H ribonuclease activity[TP5000] ExcelTaq™ Hot Start II DNA PolymeraseAptamer-based hot start PCRReversible enzyme inactivationOmits extra enzyme activation stepConvenient for room temperature PCR set-upHigh yield and specificity of target ampliconsWide range of amplicon length (up to 10 kb)High sensitivity (as low as 1 fg of plasmid)[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) High StabilityFast Hot StartHigh SensitivityLow Background / High Specificity Suitable for Fast Program Smart Blue Contrast Dye [TP1200] ExcelTaq™ 5X PCR Master Dye Mix5’→3’ DNA polymerase activityNo detectable 3"→5" exonuclease (proofreading) activityGenerates PCR products with 3"-dA overhangsHigh yield PCR High reproducibilityReduced  pipetting errorsIncludes tracking dye for direct loading after PCR