产品说明
Product overview Technical/Spec Support/Documents FAQsApplication references Related products Description The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers, TaqMan probes and templates. The master mix includes a 5’ to 3’ exonuclease activity to cleave TaqMan probes that hybridize to target sequences, releasing fluorophore during probe displacement. With TaqMan probes, the master mix features high specificity and high sensitivity. The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) contains hot-start Taq polymerase in an optimized buffer that allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for the normalization of each qPCR assay. The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.FeaturesHigh SpecificityHigh SensitivityWith ROX Reference DyeStorageProtect from light. Aliquot to avoid multiple freeze-thaw cycles. -20°C for 12 monthsHigh Sensitivity The amplification plot of real-time PCR with cDNA templates ranging from 76 fg to 20 ng in quantity, analyzed by using TQ2110 ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) for qPCR amplification.  High Efficiency The standard curve of TQ2110 ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) was generated by using 4-fold dilution of total cDNA ranging from 76 fg to 20 ng.  ContentsComponentVolumeCat. NoExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)2 x 1 mlTQ2110 (200 Rxn)Storage Protected from light. Aliquot to avoid multiple freeze-thaw cycles.-20°C for 12 months ManualManual_TQ2110_ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)SDSSDS_TQ2110FlyerSMOBIO’s Q-PCR Master Mix- Instrument Compatibility  TaqMan® vs. SYBR® Green Chemistry   How can I choose ExcelTaq™ 2X Q-PCR Master Mix?  What instruments are compatible with ExcelTaq™ 2X Q-PCR Master Mix ?  Product NameExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)Cat. No.TQ1100TQ1110TQ1200TQ1210TQ2110Detection chemistrySYBRSYBRSYBRSYBRTaqManBlue contrast dye for best convenienceüüüüXROX dye for referenceXüXüüTime required for enzyme activation10 min10 min2 min2 min10 minqPCR programStandardStandardFast and StandardFast and StandardStandardInstrument Compatibility*Applied Biosystems system:   5700, 7000, 7300, 7700, and 7900HT ΔVΔVV   StepOne, StepOnePlusΔVΔVV   7500, 7500 Fast, QuantStudio, ViiA7 ΔΔΔΔΔBioRad system:   CFX96 , CFX384VVVVV   iQ5VVVVV   Opticon, Opticon 2, Chromo 4VVVVVCepheid system:   Smart CyclerVVVVVEppendorf system:   Mastercycler ep realplex VVVVVRoche system:   LightCycler 480, NanoVVVVVQIAgen system:   Rotor-Gene™ 3000, 6000, QVVVVVAgilent (Stratagene) system:   MX3000P, 3005P, 4000PΔΔΔΔΔIllumina system:   EcoVVVVV*ExcelTaq™ 2X Q-PCR Master Mix can be used with a variety of real-time instruments, including but not limited to the list above.V– Indicates the reagent is compatible to the instrumentΔ– Indicates that if use the reagent to the instrument, ensure to de-select passive dye channel when data processing.  Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.Satnam Singh  Suneet Pandher  Mridula Gupta  Gurmeet Kaur  Pankaj RathoreJournal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056PMCID: PMC5522218Hydroxylation and sulfation of sex steroid hormones in inflammatory liver Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031PMCID: PMC5706436Loss of endogenous estrogen increases cardiac toxicity by doxorubicinYoung Ho Lee, Sang R. Lee, Sun Woo Kwon, Seung-yeon Lee, Bae-kuen Park, Eui-Ju HongJournal of Biomedical Translational Research Vol.18 No.4 pp.146-150 DOI : https://doi.org/10.12729/jbtr.2017.18.4.146 ExcelTaq™ 2X Q-PCR Master MixProduct NameExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)Cat. No.TQ1100TQ1110TQ1200TQ1210TQ2110Detection chemistrySYBRSYBRSYBRSYBRTaqManBlue contrast dye for best convenienceüüüüXROX dye for referenceXüXüüTime required for enzyme activation10 min10 min2 min2 min10 minqPCR programStandardStandardFast and StandardFast and StandardStandard

SMOBIO的B-BOX™是一种长波长蓝光LED落射照明器。它设计紧凑,结构坚固。B-BOX™落射照明器由于其非紫外线光源和低至12伏的低工作电压,并且为非致癌DNA /蛋白质染料提供了工作能力,因此为用户提供了前所未有的安全性。B-BOX™带有高质量的非闪烁LED灯,对DNA和蛋白质染料高度敏感。B-BOX™可以极大地减轻凝胶提取的日常琐事,即使在明亮的环境光下也可以轻松进行可视化和凝胶切割。多角度滤板(目录号VE0102)为凝胶切割,可视化和记录提供了最佳角度。B-BOX™还带有用于过滤和可视化的琥珀色滤镜(目录号VE0103)。Phox™Photobox(目录号 VE0104)为使用任何智能手机拍摄的图像提供了一个迷你暗室环境。最后,工作区域周围的内置屏障系统有助于清洁。




特征


提高克隆效率 


紧凑,轻巧和便携式(重量不足1千克)


安全功能包括:470 nm长波长,对用户没有任何紫外线辐射危害


与非致癌,非溴化乙锭的DNA染料兼容


用户友好:样品易于观察(使用滤板或护目镜时) 


LED光源持续长达50,000小时


出色的检测灵敏度:使用FluoroStain™DNA荧光染色染料时,DNA≤0.04 ng,使用FluoroStain™蛋白荧光染色染料时,蛋白≤3ng(与银染一样灵敏)


可调和可移动的滤板可进行凝胶切割,可视化和记录


内置屏障设计,易于清理


在明亮的环境光下可见


借助其自身的内置散热器,强调了最小的功率依赖,低发热


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