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Product overview Technical/Spec Support/Documents FAQsApplication references Related products Description The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye (Cat. No. CF1000) if recommended by the manufacturer of the qPCR system. FeaturesHigh sensitivity and signal intensitySmart blue contrast dye as a visual aid for reaction setupBetter compatibility for reverse transcriptionLow background Storage Protected from light. Aliquot to avoid multiple freeze-thaw cycles.-20°C for 12 monthsSensitivityThe amplification plot of real-time PCR with cDNA template ranged from 25 ng to 1.49 fg in quantity, analyzed by using TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) for Q-PCR amplification. Better Compatibility for reverse transcription ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) shows better compatibility with reverse-transcription reaction mixture as compared to similar products from A and B brands. Two μl of cDNA directly obtained from reverse-transcription reaction mixture were used in a 20 μl qPCR reaction for the compatibility test.Specificity The standard curve (right) and melting curve (left)  of TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX).Contents ComponentVolumeCat. No ExcelTaq™ 2X Q-PCR Master Mix(SYBR, no ROX)2 x1 mlTQ1100 (200 Rxn) ExcelTaq™ 2X Q-PCR Master Mix(SYBR, no ROX)5 x1 mlTQ1101 (500 Rxn) Storage Protected from light. Aliquot to avoid multiple freeze-thaw cycles.-20°C for 12 months ManualManual_TQ1100_ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)SDSSDS_TQ1100FlyerSMOBIO’s Q-PCR Master Mix- Instrument Compatibility   TaqMan® vs. SYBR® Green Chemistry   How can I choose ExcelTaq™ 2X Q-PCR Master Mix?  What instruments are compatible with ExcelTaq™ 2X Q-PCR Master Mix ?  Product NameExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)Cat. No.TQ1100TQ1110TQ1200TQ1210TQ2110Detection chemistrySYBRSYBRSYBRSYBRTaqManBlue contrast dye for best convenienceüüüüXROX dye for referenceXüXüüTime required for enzyme activation10 min10 min2 min2 min10 minqPCR programStandardStandardFast and StandardFast and StandardStandardInstrument Compatibility*Applied Biosystems system:   5700, 7000, 7300, 7700, and 7900HT ΔVΔVV   StepOne, StepOnePlusΔVΔVV   7500, 7500 Fast, QuantStudio, ViiA7 ΔΔΔΔΔBioRad system:   CFX96 , CFX384VVVVV   iQ5VVVVV   Opticon, Opticon 2, Chromo 4VVVVVCepheid system:   Smart CyclerVVVVVEppendorf system:   Mastercycler ep realplex VVVVVRoche system:   LightCycler 480, NanoVVVVVQIAgen system:   Rotor-Gene™ 3000, 6000, QVVVVVAgilent (Stratagene) system:   MX3000P, 3005P, 4000PΔΔΔΔΔIllumina system:   EcoVVVVV*ExcelTaq™ 2X Q-PCR Master Mix can be used with a variety of real-time instruments, including but not limited to the list above.V– Indicates the reagent is compatible to the instrumentΔ– Indicates that if use the reagent to the instrument, ensure to de-select passive dye channel when data processing. Can you provide the results that using ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX, TQ1100) with different instruments?  Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae)Satnam Singh,corresponding author Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Neha Goel, and Pankaj RathoreSci Rep. 2019; 9: 13710. Published online 2019 Sep 23. doi: 10.1038/s41598-019-49997-yPMCID: PMC6757040Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.Satnam Singh  Suneet Pandher  Mridula Gupta  Gurmeet Kaur  Pankaj RathoreJournal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056PMCID: PMC5522218Hydroxylation and sulfation of sex steroid hormones in inflammatory liver Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031PMCID: PMC5706436Loss of endogenous estrogen increases cardiac toxicity by doxorubicinYoung Ho Lee, Sang R. Lee, Sun Woo Kwon, Seung-yeon Lee, Bae-kuen Park, Eui-Ju HongJournal of Biomedical Translational Research Vol.18 No.4 pp.146-150 DOI : https://doi.org/10.12729/jbtr.2017.18.4.146ExcelTaq™ 2X Q-PCR Master Mix Product NameExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) Cat. No.TQ1100TQ1110TQ1200TQ1210TQ2110 Detection chemistrySYBRSYBRSYBRSYBRTaqMan Blue contrast dye for best convenienceüüüüX ROX dye for referenceXüXüü Time required for enzyme activation10min10min2min2min10min qPCR programStandardStandardFastand StandardFastand StandardStandard

SMOBIO的B-BOX™是一种长波长蓝光LED落射照明器。它设计紧凑,结构坚固。B-BOX™落射照明器由于其非紫外线光源和低至12伏的低工作电压,并且为非致癌DNA /蛋白质染料提供了工作能力,因此为用户提供了前所未有的安全性。B-BOX™带有高质量的非闪烁LED灯,对DNA和蛋白质染料高度敏感。B-BOX™可以极大地减轻凝胶提取的日常琐事,即使在明亮的环境光下也可以轻松进行可视化和凝胶切割。多角度滤板(目录号VE0102)为凝胶切割,可视化和记录提供了最佳角度。B-BOX™还带有用于过滤和可视化的琥珀色滤镜(目录号VE0103)。Phox™Photobox(目录号 VE0104)为使用任何智能手机拍摄的图像提供了一个迷你暗室环境。最后,工作区域周围的内置屏障系统有助于清洁。




特征


提高克隆效率 


紧凑,轻巧和便携式(重量不足1千克)


安全功能包括:470 nm长波长,对用户没有任何紫外线辐射危害


与非致癌,非溴化乙锭的DNA染料兼容


用户友好:样品易于观察(使用滤板或护目镜时) 


LED光源持续长达50,000小时


出色的检测灵敏度:使用FluoroStain™DNA荧光染色染料时,DNA≤0.04 ng,使用FluoroStain™蛋白荧光染色染料时,蛋白≤3ng(与银染一样灵敏)


可调和可移动的滤板可进行凝胶切割,可视化和记录


内置屏障设计,易于清理


在明亮的环境光下可见


借助其自身的内置散热器,强调了最小的功率依赖,低发热


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