首页 > haemtech > Haematologic Technologies/CTI vacuum collection tube/SCAT-27/25 X 2 mL, 25 X 3 mL, 25 X 5 mL

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Haematologic Technologies/CTI vacuum collection tube/SCAT-27/25 X 2 mL, 25 X 3 mL, 25 X 5 mL

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Formulation11mMCitrate,50ug/mLCTI(final)MolecularWeightStorage4°CPurityN/ACompoundAssayN/AShelfLife(properlystored)6monthsChemicalFormulaEffectofCTIontheAPTTandPTofNormalHumanPlasmaThegraphillustratestheeffectofaddedCTIonthePTandaPTTofnormalhumanplasma.CTIwasaddedtonormalpooledhumanplasmatovariousfinalconcentrations,andthemixtureswereallowedtoincubateforabriefperiodonicepriortoconductingtheassays.ThePTremainsunaffectedwhiletheaPTTdoubleswiththeadditionofapproximately60microgramsofCTIpermlofplasma.SampleGelInformation:GelNovex4-12%Bis-TrisLoadCornTrypsinInhibitor,1µgperlaneBufferMESStandardSeeBluePlus2;Myosin(188kDa),PhosphorylaseB(98kDa),BSA(62kDa),GlutamicDehydrogenase(49kDa),AlcoholDehydrogenase(38kDa),CarbonicAnhydrase(28kDa),MyoglobinRed(17kDa),Lysozyme(14kDa),Aprotinin(6kDa),Insulin,Bchain(3kDa).Overview:PurifiedCTICorntrypsininhibitor(CTI)isasmallproteinthatislocalizedinthekernelsofmostspeciesofcorn.CTIisnotonlyaninhibitoroftrypsin,butisalsoaspecifichumanfactorXIIainhibitorwhentestedinbloodclottingexperiments(1-6).Theinhibitorformsaone-to-onecomplexwitheithertrypsinorfactorXIIa,andwhenaddedtoplasma,prolongstheactivatedpartialthromboplastintimewithoutaffectingthePTassay(6).ThespecificityforfactorXIIamakestheinhibitorusefulforthesegregationandstudyoftissuefactor(TF)dependentcoagulationreactions(6).TheuseofCTItostudyTF-dependentreactionshasbeendocumentedinliterature(6,8-10).StudiesindicatethatsuppressionofthecontactpathwayofcoagulationisessentialwhenattemptingtoperformTF-dependentassaysinwholebloodorplasmasamples.TheadditionofCTIatthepointofsamplecollectionpreventsactivationofthecontactpathwayduringsubsequentsampleprocessingsteps,thusreducingin-vitroartifact.ThemostcommonuseofCTIisassociatedwiththrombingenerationassayswhenattemptingtoworkatlowTFconcentrations.HTI’sCTIproductisperformancetestedforthisspecificapplication.Theproteiniscomprisedof112aminoacidswhichyieldsacalculatedmolecularweightof12,028(7).Thecalculatedmolecularweightandthatwhichhasbeendeterminedbysedimentationequilibriumanalysesofthepurifiedprotein(12,500)areingoodagreement(3).AcomparisonoftheaminoacidsequenceofCTItothatofothertrypsinorserineproteaseinhibitorsrevealslittleornosimilarities(7).CTIispurifiedusingacombinationofpublishedprocedures(3,4).Tobeginthepurificationprocess,CTIisextractedfromthekernelsoffreshsweetcornintoaphysiologicbuffer.Theextractisthende-fattedusingacetone,andtheproteinisfurtherpurifiedbyemployinggelfiltrationandion-exchangechromatography.ThefinalCTIpreparationappearsasasinglebandbySDS-PAGEanalysesunderbothreducingandnon-reducingconditions.PreparationsofCTIaretestedfortheABIlitytoprolongtheaPTTassaywithoutaffectingthePTassay.ThespecificactivityofeachlotofCTIisdetermined,andoneunitisdefinedastheamountofCTIrequiredtodoubletheaPTTofnormalhumanplasma.PurifiedCTIisformulatedin20mMTris,0.15MNaCl,pH7.4andshouldbestoredfrozenat-20°Corcolder.BloodCollectionTubesTosimplifytheprocessofcollectingsampleswithaddedCTI,HTIhasdevelopedbloodcollectiontubescontainingCTI.Thesetubes(ourSCAT-27line)simplifytheprocessofconductingTF-dependentstudiesbyallowingyoutodrawblooddirectlyontoananticoagulantcontainingCTI.YoumaychoosetouseourstandardCTI/Citrateformulation(11mMCitrate,50ug/mLCTI)oryoumaycreateyourowncustomformulation.HTI’sbloodcollectiontubesarenotsterileandaremanufacturedandsoldforresearchuseonly.Threestandardsizesareavailablealthoughcustomsizescanbemanufacturedforyou.SCAT-27-4.5/5-5mLtubeSCAT-27-2.7/5-3mLtubeSCAT-27-1.8/5-2mLtube Properties:LocalizationCornKernalsModeofactionformsaone-to-onecomplexwitheithertrypsinorfactorXIIaandinhibitstheirrespectivecatalyticactivity.Molecularweight12,500(3)ExtinctioncoefficientE1%1cm,280nm=20.0(determinedbyultracentrifugationstudies)Structuresinglechainproteincomprisedof112aminoacids(3,7)

Haematologic Technologies是一家通过ISO 9001：2015认证的公司，是一家主要制造商，专门从事旨在用于体外研究的高质量血浆蛋白的分离和表征的主要制造商。HT的重点是参与凝血级联反应的蛋白质，以及骨代谢的调节。HT产品系列包括150多种高度纯化且特性良好的蛋白质，包括酶原，酶，辅因子和抑制剂，以及单克隆和多克隆抗体的互补产品系列。还提供因子不足的血浆和定制的用于临床研究的定制采血管。提供的服务包括定制蛋白质纯化，蛋白质修饰，分析开发，合同制造和合同研究。