β-GalactosidaseEnzymeAssaySystemwithReporterLysisBuffer β-Galactosidaseisacommonlyusedreportermolecule.Theβ-GalactosidaseEnzymeAssaySystemwithReporterLysisBuffer(Cat.#E2000)isaconvenientmethodforassayingβ-galactosidaseactivityinlysatespreparedfromcellstransfectedwithβ-galactosidasereportervectorssuchasPromega"spSV-β-GalactosidaseControlVector. ThestandardassayisperformedbyaddingadilutesampletoanequalvolumeofAssay2XBuffer,whichcontainsthesubstrateONPG(o-nitrophenyl-beta-D-galactopyranoside).Samplesareincubatedfor30minutes,duringwhichtimetheBeta-Galactosidasehydrolyzesthecolorlesssubstratetoo-nitrophenyl,whichisyellow.Thereactionisterminatedbyadditionofsodiumcarbonate,andtheabsorbanceisreadat420nmwithaspectrophotometer. 点击浏览该文件