用DAPI进行细胞染色DAPI的配置Stocksolutionof1mg/mlinddH2O;workingsolutionof0.25mg/mlto50mg/mlinddH2OorPGM.1.Placesampleonslide.2.Addafewdropsworkingsolution.3.Squashandview.Staining–DAPIStainingforCells:SeparatelyDAPIstaining:•WashthecellstwicewithPBS•Incubatewith1:750ofstockDAPIfor5minindark•Wash2x5minwithPBSindark•Rinsewithwaterforafewseconds•AirdryatRTindark•Mountwithantifadesolution(Vectashield,Co#:H-1000,VectorLab)5µlforeachd12mmcoverslip,25µlforsquarecoverslipand50µlforfullsizecoverslip•Sealthecoverslipwithnailpolish•Storein4Cupto2weeksDAPIstock•4’,6-Diamidino-2-phenylindoledihydrochloridehydrate(DAPI)fromSigma(Cat#D-9542),1mg1mg/0.5mlinH2Oandstockedat–20inthedarkStainingwithantifademountingsolution:•WashthecellstwicewithPBS•MountwithantifadesolutionwithDAPI(Vectashield,Co#:H-1200，VectorLab,1.5µg/ml)at1:3dilutionwithVectashieldH-1000Volumeusedasabove•Storein4Cupto2weeks
